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  • 2016_ET_DEHP apoptosis in GC-2spd cells

    Rights statement: This is the peer reviewed version of the following article: Fu, G., Dai, J., Zhang, D., Zhu, L., Tang, X., Zhang, L., Zhou, T., Duan, P., Quan, C., Zhang, Z., Song, S. and Shi, Y. (2017), Di(2-ethylhexyl) phthalate induces apoptosis through mitochondrial pathway in GC-2spd cells. Environ. Toxicol., 32: 1055–1064. doi:10.1002/tox.22304 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/tox.22304/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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    Available under license: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License

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Di(2-ethylhexyl) phthalate induces apoptosis through mitochondrial pathway in GC-2spd cells

Research output: Contribution to journalJournal article

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  • Guoqing Fu
  • Juan Dai
  • Dayi Zhang
  • Lishan Zhu
  • Xiao Tang
  • Ling Zhang
  • Ting Zhou
  • Peng Duan
  • Chao Quan
  • Zhibing Zhang
  • Shizhen Song
  • Yuqin Shi
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<mark>Journal publication date</mark>03/2017
<mark>Journal</mark>Environmental Toxicology
Issue number3
Volume32
Number of pages10
Pages (from-to)1055-1064
Publication statusPublished
Early online date14/07/16
Original languageEnglish

Abstract

Di(2-ethylhexyl) phthalate (DEHP), a plasticizer of synthetic polymers, is a well-known endocrine disrupting chemical (EDC) and reproductive toxicant. Addressing the unclear mechanism of DEHP-induced reproductive dysfunction, this study used GC-2spd cells to investigate the molecular mechanism involved in the DEHP-induced toxicity in the male reproductive system. The results indicated that the apoptotic cell death was significantly induced by DEHP exposure over 100 μM. Furthermore, DEHP treatment could induce oxidative stress in GC-2spd cells involving in the decrease of superoxide dismutase (SOD) activity (200 μM) and glutathione peroxidase (GSH-Px) activity (50 and 100 μM). In addition, DEHP induction also caused the elevated ratios of Bax/Bcl-2, release of cytochrome c and decomposition of procaspase-3 and procaspase-9 in GC-2spd cells. Taken together, our work provided the evidence that DEHP exposure might induce apoptosis of GC-2spd cells via mitochondria pathway mediated by oxidative stress. © 2016 Wiley Periodicals, Inc. Environ Toxicol, 2016.

Bibliographic note

This is the peer reviewed version of the following article: Fu, G., Dai, J., Zhang, D., Zhu, L., Tang, X., Zhang, L., Zhou, T., Duan, P., Quan, C., Zhang, Z., Song, S. and Shi, Y. (2017), Di(2-ethylhexyl) phthalate induces apoptosis through mitochondrial pathway in GC-2spd cells. Environ. Toxicol., 32: 1055–1064. doi:10.1002/tox.22304 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/tox.22304/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.