Home > Research > Publications & Outputs > Direct effects of snowdrop lectin (GNA) on larv...
View graph of relations

Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion. / Hogervorst, Petra A. M.; Ferry, Natalie; Gatehouse, Angharad M. R. et al.
In: Journal of Insect Physiology, Vol. 52, No. 6, 04.2006, p. 614-624.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Hogervorst, PAM, Ferry, N, Gatehouse, AMR, Wäckers, FL & Romeis, J 2006, 'Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion.', Journal of Insect Physiology, vol. 52, no. 6, pp. 614-624. https://doi.org/10.1016/j.jinsphys.2006.02.011

APA

Hogervorst, P. A. M., Ferry, N., Gatehouse, A. M. R., Wäckers, F. L., & Romeis, J. (2006). Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion. Journal of Insect Physiology, 52(6), 614-624. https://doi.org/10.1016/j.jinsphys.2006.02.011

Vancouver

Hogervorst PAM, Ferry N, Gatehouse AMR, Wäckers FL, Romeis J. Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion. Journal of Insect Physiology. 2006 Apr;52(6):614-624. doi: 10.1016/j.jinsphys.2006.02.011

Author

Hogervorst, Petra A. M. ; Ferry, Natalie ; Gatehouse, Angharad M. R. et al. / Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion. In: Journal of Insect Physiology. 2006 ; Vol. 52, No. 6. pp. 614-624.

Bibtex

@article{3d44412320f745d2b3dc0a44b74bb0dd,
title = "Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion.",
abstract = "Plants genetically modified to express Galanthus nivalis agglutinin (GNA) have been found to confer partial resistance to homopteran pests. Laboratory experiments were conducted to investigate direct effects of GNA on larvae of three species of aphid predators that differ in their feeding and digestive physiology, i.e. Chrysoperla carnea, Adalia bipunctata and Coccinella septempunctata. Longevity of all three predator species was directly affected by GNA, when they were fed a sucrose solution containing 1% GNA. However, a difference in sensitivity towards GNA was observed when comparing the first and last larval stage of the three species. In vitro studies revealed that gut enzymes from none of the three species were able to break down GNA. In vivo feed-chase studies demonstrated accumulation of GNA in the larvae. After the larvae had been transferred to a diet devoid of GNA, the protein stayed present in the body of C. carnea, but decreased over time in both ladybirds. Binding studies showed that GNA binds to glycoproteins that can be found in the guts of larvae of all three predator species. Immunoassay by Western blotting of haemolymph samples only occasionally showed the presence of GNA. Fluorescence microscopy confirmed GNA accumulation in the midgut of C. carnea larvae. Implications of these findings for non-target risk assessment of GNA-transgenic crops are discussed.",
keywords = "Galanthus nivalis agglutinin, Chrysoperla carnea, Adalia bipunctata, Coccinella septempunctata, Non-target effects, Risk assessment",
author = "Hogervorst, {Petra A. M.} and Natalie Ferry and Gatehouse, {Angharad M. R.} and W{\"a}ckers, {Felix L.} and J{\"o}rg Romeis",
year = "2006",
month = apr,
doi = "10.1016/j.jinsphys.2006.02.011",
language = "English",
volume = "52",
pages = "614--624",
journal = "Journal of Insect Physiology",
issn = "0022-1910",
publisher = "Elsevier Limited",
number = "6",

}

RIS

TY - JOUR

T1 - Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion.

AU - Hogervorst, Petra A. M.

AU - Ferry, Natalie

AU - Gatehouse, Angharad M. R.

AU - Wäckers, Felix L.

AU - Romeis, Jörg

PY - 2006/4

Y1 - 2006/4

N2 - Plants genetically modified to express Galanthus nivalis agglutinin (GNA) have been found to confer partial resistance to homopteran pests. Laboratory experiments were conducted to investigate direct effects of GNA on larvae of three species of aphid predators that differ in their feeding and digestive physiology, i.e. Chrysoperla carnea, Adalia bipunctata and Coccinella septempunctata. Longevity of all three predator species was directly affected by GNA, when they were fed a sucrose solution containing 1% GNA. However, a difference in sensitivity towards GNA was observed when comparing the first and last larval stage of the three species. In vitro studies revealed that gut enzymes from none of the three species were able to break down GNA. In vivo feed-chase studies demonstrated accumulation of GNA in the larvae. After the larvae had been transferred to a diet devoid of GNA, the protein stayed present in the body of C. carnea, but decreased over time in both ladybirds. Binding studies showed that GNA binds to glycoproteins that can be found in the guts of larvae of all three predator species. Immunoassay by Western blotting of haemolymph samples only occasionally showed the presence of GNA. Fluorescence microscopy confirmed GNA accumulation in the midgut of C. carnea larvae. Implications of these findings for non-target risk assessment of GNA-transgenic crops are discussed.

AB - Plants genetically modified to express Galanthus nivalis agglutinin (GNA) have been found to confer partial resistance to homopteran pests. Laboratory experiments were conducted to investigate direct effects of GNA on larvae of three species of aphid predators that differ in their feeding and digestive physiology, i.e. Chrysoperla carnea, Adalia bipunctata and Coccinella septempunctata. Longevity of all three predator species was directly affected by GNA, when they were fed a sucrose solution containing 1% GNA. However, a difference in sensitivity towards GNA was observed when comparing the first and last larval stage of the three species. In vitro studies revealed that gut enzymes from none of the three species were able to break down GNA. In vivo feed-chase studies demonstrated accumulation of GNA in the larvae. After the larvae had been transferred to a diet devoid of GNA, the protein stayed present in the body of C. carnea, but decreased over time in both ladybirds. Binding studies showed that GNA binds to glycoproteins that can be found in the guts of larvae of all three predator species. Immunoassay by Western blotting of haemolymph samples only occasionally showed the presence of GNA. Fluorescence microscopy confirmed GNA accumulation in the midgut of C. carnea larvae. Implications of these findings for non-target risk assessment of GNA-transgenic crops are discussed.

KW - Galanthus nivalis agglutinin

KW - Chrysoperla carnea

KW - Adalia bipunctata

KW - Coccinella septempunctata

KW - Non-target effects

KW - Risk assessment

U2 - 10.1016/j.jinsphys.2006.02.011

DO - 10.1016/j.jinsphys.2006.02.011

M3 - Journal article

VL - 52

SP - 614

EP - 624

JO - Journal of Insect Physiology

JF - Journal of Insect Physiology

SN - 0022-1910

IS - 6

ER -