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Diversity in repeat-containing surface proteins of Leishmania major.

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Diversity in repeat-containing surface proteins of Leishmania major. / McKean, Paul G.; Trenholme, Katharine R.; Rangarajan, Desikan; Keen, Jane K.; Smith, Deborah F.

In: Molecular and Biochemical Parasitology, Vol. 86, No. 2, 06.1997, p. 225-235.

Research output: Contribution to journalJournal article

Harvard

McKean, PG, Trenholme, KR, Rangarajan, D, Keen, JK & Smith, DF 1997, 'Diversity in repeat-containing surface proteins of Leishmania major.', Molecular and Biochemical Parasitology, vol. 86, no. 2, pp. 225-235. https://doi.org/10.1016/S0166-6851(97)00035-2

APA

McKean, P. G., Trenholme, K. R., Rangarajan, D., Keen, J. K., & Smith, D. F. (1997). Diversity in repeat-containing surface proteins of Leishmania major. Molecular and Biochemical Parasitology, 86(2), 225-235. https://doi.org/10.1016/S0166-6851(97)00035-2

Vancouver

McKean PG, Trenholme KR, Rangarajan D, Keen JK, Smith DF. Diversity in repeat-containing surface proteins of Leishmania major. Molecular and Biochemical Parasitology. 1997 Jun;86(2):225-235. https://doi.org/10.1016/S0166-6851(97)00035-2

Author

McKean, Paul G. ; Trenholme, Katharine R. ; Rangarajan, Desikan ; Keen, Jane K. ; Smith, Deborah F. / Diversity in repeat-containing surface proteins of Leishmania major. In: Molecular and Biochemical Parasitology. 1997 ; Vol. 86, No. 2. pp. 225-235.

Bibtex

@article{14085d8276ab424aa7149f29555357f1,
title = "Diversity in repeat-containing surface proteins of Leishmania major.",
abstract = "The gene B protein (GBP) is one of the products of the LmcDNA16 gene family, a cluster of related but non-identical genes that are differentially-expressed during the Leishmania life cycle. This protein, which is found on the surface of infective stage parasites, contains an extensive region of proline-rich amino acid repeats, constituting 45{\%} of the total protein. The structure and stability of these repeats have been investigated in a number of L. major strains by polymerase chain reaction (PCR) amplification and Southern blotting. Data reported in this paper demonstrate variability between strains with respect to the number of repeats encoded by GBP, although those strains isolated within adjacent geographical regions have conserved repeat structures. The data also reveal that some parasite lines have additional repeat sequences within a second, related gene in the LmcDNA16 array. Western blotting experiments have established that these sequences are expressed in vivo, indicating that L. major strains are heterogeneous in their surface complement of gene B repeat-containing proteins.",
keywords = "Leishmania major, Surface proteins, Amino acid repeats, Diversity",
author = "McKean, {Paul G.} and Trenholme, {Katharine R.} and Desikan Rangarajan and Keen, {Jane K.} and Smith, {Deborah F.}",
year = "1997",
month = "6",
doi = "10.1016/S0166-6851(97)00035-2",
language = "English",
volume = "86",
pages = "225--235",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Diversity in repeat-containing surface proteins of Leishmania major.

AU - McKean, Paul G.

AU - Trenholme, Katharine R.

AU - Rangarajan, Desikan

AU - Keen, Jane K.

AU - Smith, Deborah F.

PY - 1997/6

Y1 - 1997/6

N2 - The gene B protein (GBP) is one of the products of the LmcDNA16 gene family, a cluster of related but non-identical genes that are differentially-expressed during the Leishmania life cycle. This protein, which is found on the surface of infective stage parasites, contains an extensive region of proline-rich amino acid repeats, constituting 45% of the total protein. The structure and stability of these repeats have been investigated in a number of L. major strains by polymerase chain reaction (PCR) amplification and Southern blotting. Data reported in this paper demonstrate variability between strains with respect to the number of repeats encoded by GBP, although those strains isolated within adjacent geographical regions have conserved repeat structures. The data also reveal that some parasite lines have additional repeat sequences within a second, related gene in the LmcDNA16 array. Western blotting experiments have established that these sequences are expressed in vivo, indicating that L. major strains are heterogeneous in their surface complement of gene B repeat-containing proteins.

AB - The gene B protein (GBP) is one of the products of the LmcDNA16 gene family, a cluster of related but non-identical genes that are differentially-expressed during the Leishmania life cycle. This protein, which is found on the surface of infective stage parasites, contains an extensive region of proline-rich amino acid repeats, constituting 45% of the total protein. The structure and stability of these repeats have been investigated in a number of L. major strains by polymerase chain reaction (PCR) amplification and Southern blotting. Data reported in this paper demonstrate variability between strains with respect to the number of repeats encoded by GBP, although those strains isolated within adjacent geographical regions have conserved repeat structures. The data also reveal that some parasite lines have additional repeat sequences within a second, related gene in the LmcDNA16 array. Western blotting experiments have established that these sequences are expressed in vivo, indicating that L. major strains are heterogeneous in their surface complement of gene B repeat-containing proteins.

KW - Leishmania major

KW - Surface proteins

KW - Amino acid repeats

KW - Diversity

U2 - 10.1016/S0166-6851(97)00035-2

DO - 10.1016/S0166-6851(97)00035-2

M3 - Journal article

VL - 86

SP - 225

EP - 235

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 2

ER -