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Diversity of gut microbiota increases with aging and starvation in the desert locust

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Diversity of gut microbiota increases with aging and starvation in the desert locust. / Dillon, Rod J.; Webster, Gordon; Weightman, Andrew J. et al.
In: Antonie van Leeuwenhoek, Vol. 97, No. 1, 01.2010, p. 69-77.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Dillon, RJ, Webster, G, Weightman, AJ & Charnley, AK 2010, 'Diversity of gut microbiota increases with aging and starvation in the desert locust', Antonie van Leeuwenhoek, vol. 97, no. 1, pp. 69-77. https://doi.org/10.1007/s10482-009-9389-5

APA

Dillon, R. J., Webster, G., Weightman, A. J., & Charnley, A. K. (2010). Diversity of gut microbiota increases with aging and starvation in the desert locust. Antonie van Leeuwenhoek, 97(1), 69-77. https://doi.org/10.1007/s10482-009-9389-5

Vancouver

Dillon RJ, Webster G, Weightman AJ, Charnley AK. Diversity of gut microbiota increases with aging and starvation in the desert locust. Antonie van Leeuwenhoek. 2010 Jan;97(1):69-77. doi: 10.1007/s10482-009-9389-5

Author

Dillon, Rod J. ; Webster, Gordon ; Weightman, Andrew J. et al. / Diversity of gut microbiota increases with aging and starvation in the desert locust. In: Antonie van Leeuwenhoek. 2010 ; Vol. 97, No. 1. pp. 69-77.

Bibtex

@article{4764d29aa05c4249ac44cdeaedbc7d91,
title = "Diversity of gut microbiota increases with aging and starvation in the desert locust",
abstract = "Here we report the effects of starvation and insect age on the diversity of gut microbiota of adult desert locusts, Schistocerca gregaria, using denaturing gradient gel electrophoretic (DGGE) analysis of bacterial 16S rRNA genes. Sequencing of excised DGGE bands revealed the presence of only one potentially novel uncultured member of the Gammaproteobacteria in the guts of fed, starved, young or old locusts. Most of the 16S rRNA gene sequences were closely related to known cultured bacterial species. DGGE profiles suggested that bacterial diversity increased with insect age and did not provide evidence for a characteristic locust gut bacterial community. Starved insects are often more prone to disease, probably because they compromise on immune defence. However, the increased diversity of Gammaproteobacteria in starved locusts shown here may improve defence against enteric threats because of the role of gut bacteria in colonization resistance.",
keywords = "DGGE, 16S rRNA genes, Acrididae, Bacteria, Gut, BACTERIAL COMMUNITY STRUCTURE, 16S RIBOSOMAL-RNA, SCHISTOCERCA-GREGARIA, MANAGEMENT REGIMENS, POPULATIONS, MIDGUT, FLORA, IDENTIFICATION, DROSOPHILA, MUTUALISM",
author = "Dillon, {Rod J.} and Gordon Webster and Weightman, {Andrew J.} and Charnley, {A. Keith}",
year = "2010",
month = jan,
doi = "10.1007/s10482-009-9389-5",
language = "English",
volume = "97",
pages = "69--77",
journal = "Antonie van Leeuwenhoek",
issn = "0003-6072",
publisher = "Springer Netherlands",
number = "1",

}

RIS

TY - JOUR

T1 - Diversity of gut microbiota increases with aging and starvation in the desert locust

AU - Dillon, Rod J.

AU - Webster, Gordon

AU - Weightman, Andrew J.

AU - Charnley, A. Keith

PY - 2010/1

Y1 - 2010/1

N2 - Here we report the effects of starvation and insect age on the diversity of gut microbiota of adult desert locusts, Schistocerca gregaria, using denaturing gradient gel electrophoretic (DGGE) analysis of bacterial 16S rRNA genes. Sequencing of excised DGGE bands revealed the presence of only one potentially novel uncultured member of the Gammaproteobacteria in the guts of fed, starved, young or old locusts. Most of the 16S rRNA gene sequences were closely related to known cultured bacterial species. DGGE profiles suggested that bacterial diversity increased with insect age and did not provide evidence for a characteristic locust gut bacterial community. Starved insects are often more prone to disease, probably because they compromise on immune defence. However, the increased diversity of Gammaproteobacteria in starved locusts shown here may improve defence against enteric threats because of the role of gut bacteria in colonization resistance.

AB - Here we report the effects of starvation and insect age on the diversity of gut microbiota of adult desert locusts, Schistocerca gregaria, using denaturing gradient gel electrophoretic (DGGE) analysis of bacterial 16S rRNA genes. Sequencing of excised DGGE bands revealed the presence of only one potentially novel uncultured member of the Gammaproteobacteria in the guts of fed, starved, young or old locusts. Most of the 16S rRNA gene sequences were closely related to known cultured bacterial species. DGGE profiles suggested that bacterial diversity increased with insect age and did not provide evidence for a characteristic locust gut bacterial community. Starved insects are often more prone to disease, probably because they compromise on immune defence. However, the increased diversity of Gammaproteobacteria in starved locusts shown here may improve defence against enteric threats because of the role of gut bacteria in colonization resistance.

KW - DGGE

KW - 16S rRNA genes

KW - Acrididae

KW - Bacteria

KW - Gut

KW - BACTERIAL COMMUNITY STRUCTURE

KW - 16S RIBOSOMAL-RNA

KW - SCHISTOCERCA-GREGARIA

KW - MANAGEMENT REGIMENS

KW - POPULATIONS

KW - MIDGUT

KW - FLORA

KW - IDENTIFICATION

KW - DROSOPHILA

KW - MUTUALISM

U2 - 10.1007/s10482-009-9389-5

DO - 10.1007/s10482-009-9389-5

M3 - Journal article

VL - 97

SP - 69

EP - 77

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

SN - 0003-6072

IS - 1

ER -