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Dynamic changes in functional gene copy numbers and microbial communities during degradation of pyrene in soils.

Research output: Contribution to journalJournal article

Published

  • Jing-Jing Peng
  • Chao Cai
  • Min Qiao
  • Hong Li
  • Yong-Guan Zhu
Journal publication date09/2010
JournalEnvironmental Pollution
Journal number9
Volume158
Number of pages8
Pages2872-2879
Original languageEnglish

Abstract

This study investigates the dynamics of pyrene degradation rates, microbial communities, and functional gene copy numbers during the incubation of pyrene-spiked soils. Spiking pyrene to the soil was found to have negligible effects on the bacterial community present. Our results demonstrated that there was a signi fi cant difference in nidA gene copy numbers between sampling dates in QZ soil. Mycobacterium 16S rDNA clone libraries showed that more than 90% mycobacteria detected were closely related to fast- growing PAH-degrading Mycobacterium in pyrene-spiked soil, while other sequences related to slow-growing Mycobacterium were only detected in the control soil. It is suggested that nidA gene copy number and fast-growing PAH-degrading Mycobacterium could be used as indicators to predict pyrene contamination and its degradation activity in soils.