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  • Tsintzas et al APJ Endo 2020

    Rights statement: Copyright © 2020, American Journal of Physiology-Endocrinology and Metabolism

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    Embargo ends: 7/01/21

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Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight and obese men

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Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight and obese men. / Tsintzas, Kostas; Jones, Robert ; Pabla, Pardeep; Mallinson, Joanne; Barrett, David; Kim, Dong-Hyun; Cooper, Scott; Davies, Amanda; Taylor, Tariq; Gaffney, Christopher; Chee, Carolyn; van Loon, Luc; Stephens, Francis.

In: American Journal of Physiology - Endocrinology and Metabolism, 07.01.2020.

Research output: Contribution to journalJournal article

Harvard

Tsintzas, K, Jones, R, Pabla, P, Mallinson, J, Barrett, D, Kim, D-H, Cooper, S, Davies, A, Taylor, T, Gaffney, C, Chee, C, van Loon, L & Stephens, F 2020, 'Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight and obese men', American Journal of Physiology - Endocrinology and Metabolism. https://doi.org/10.1152/ajpendo.00344.2019

APA

Tsintzas, K., Jones, R., Pabla, P., Mallinson, J., Barrett, D., Kim, D-H., Cooper, S., Davies, A., Taylor, T., Gaffney, C., Chee, C., van Loon, L., & Stephens, F. (2020). Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight and obese men. American Journal of Physiology - Endocrinology and Metabolism. https://doi.org/10.1152/ajpendo.00344.2019

Vancouver

Tsintzas K, Jones R, Pabla P, Mallinson J, Barrett D, Kim D-H et al. Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight and obese men. American Journal of Physiology - Endocrinology and Metabolism. 2020 Jan 7. https://doi.org/10.1152/ajpendo.00344.2019

Author

Tsintzas, Kostas ; Jones, Robert ; Pabla, Pardeep ; Mallinson, Joanne ; Barrett, David ; Kim, Dong-Hyun ; Cooper, Scott ; Davies, Amanda ; Taylor, Tariq ; Gaffney, Christopher ; Chee, Carolyn ; van Loon, Luc ; Stephens, Francis. / Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight and obese men. In: American Journal of Physiology - Endocrinology and Metabolism. 2020.

Bibtex

@article{524d7c8aa462446e9ab4c6a412012d39,
title = "Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight and obese men",
abstract = "Muscle anabolic resistance to dietary protein is associated with obesity and insulin resistance. However, the contribution of excess consumption of fat to anabolic resistance is not well studied. The aim of these studies was to test the hypothesis that acute and short-term dietary fat overload will impair the skeletal muscle protein synthetic response to dietary protein ingestion. Eight overweight/obese males [46.4±1.4 years, BMI 32.3±5.4 kg/m2] participated in the acute feeding study, which consisted of 2 randomised crossover trials. On each occasion, subjects ingested an oral meal (with and without fat emulsion) 4h before the coingestion of milk protein, intrinsically labelled with [1-13C]phenylalanine, and dextrose. Nine overweight/obese males [44.0±1.7 years, BMI 30.1±1.1 kg/m2] participated in the chronic study, which consisted of a baseline 1-week isocaloric diet followed by a 2-week high fat diet (+25% energy excess). Acutely, incorporation of dietary amino acids into the skeletal muscle was 2-fold higher (P<0.05) in the lipid trial compared to control. There was no effect of prior lipid ingestion on indices of insulin sensitivity (muscle glucose uptake, PDC activity and Akt phosphorylation) in response to the protein/dextrose drink. Fat overfeeding had no effect on muscle protein synthesis or glucose disposal in response to whey protein ingestion, despite increased muscle DAG C16:0 (P=0.06) and ceramide C16:0 (P<0.01) levels. Neither acute nor short-term dietary fat overload has a detrimental effect on skeletal muscle protein synthetic response to dietary protein ingestion in overweight/obese men, suggesting dietary-induced accumulation of intramuscular lipids per se is not associated with anabolic resistance.",
keywords = "dietary fat, obesity, postprandial period, skeletal muscle protein synthesis, intramuscular lipids",
author = "Kostas Tsintzas and Robert Jones and Pardeep Pabla and Joanne Mallinson and David Barrett and Dong-Hyun Kim and Scott Cooper and Amanda Davies and Tariq Taylor and Christopher Gaffney and Carolyn Chee and {van Loon}, Luc and Francis Stephens",
note = "Copyright {\textcopyright} 2020, American Journal of Physiology-Endocrinology and Metabolism",
year = "2020",
month = jan
day = "7",
doi = "10.1152/ajpendo.00344.2019",
language = "English",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "1522-1555",

}

RIS

TY - JOUR

T1 - Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight and obese men

AU - Tsintzas, Kostas

AU - Jones, Robert

AU - Pabla, Pardeep

AU - Mallinson, Joanne

AU - Barrett, David

AU - Kim, Dong-Hyun

AU - Cooper, Scott

AU - Davies, Amanda

AU - Taylor, Tariq

AU - Gaffney, Christopher

AU - Chee, Carolyn

AU - van Loon, Luc

AU - Stephens, Francis

N1 - Copyright © 2020, American Journal of Physiology-Endocrinology and Metabolism

PY - 2020/1/7

Y1 - 2020/1/7

N2 - Muscle anabolic resistance to dietary protein is associated with obesity and insulin resistance. However, the contribution of excess consumption of fat to anabolic resistance is not well studied. The aim of these studies was to test the hypothesis that acute and short-term dietary fat overload will impair the skeletal muscle protein synthetic response to dietary protein ingestion. Eight overweight/obese males [46.4±1.4 years, BMI 32.3±5.4 kg/m2] participated in the acute feeding study, which consisted of 2 randomised crossover trials. On each occasion, subjects ingested an oral meal (with and without fat emulsion) 4h before the coingestion of milk protein, intrinsically labelled with [1-13C]phenylalanine, and dextrose. Nine overweight/obese males [44.0±1.7 years, BMI 30.1±1.1 kg/m2] participated in the chronic study, which consisted of a baseline 1-week isocaloric diet followed by a 2-week high fat diet (+25% energy excess). Acutely, incorporation of dietary amino acids into the skeletal muscle was 2-fold higher (P<0.05) in the lipid trial compared to control. There was no effect of prior lipid ingestion on indices of insulin sensitivity (muscle glucose uptake, PDC activity and Akt phosphorylation) in response to the protein/dextrose drink. Fat overfeeding had no effect on muscle protein synthesis or glucose disposal in response to whey protein ingestion, despite increased muscle DAG C16:0 (P=0.06) and ceramide C16:0 (P<0.01) levels. Neither acute nor short-term dietary fat overload has a detrimental effect on skeletal muscle protein synthetic response to dietary protein ingestion in overweight/obese men, suggesting dietary-induced accumulation of intramuscular lipids per se is not associated with anabolic resistance.

AB - Muscle anabolic resistance to dietary protein is associated with obesity and insulin resistance. However, the contribution of excess consumption of fat to anabolic resistance is not well studied. The aim of these studies was to test the hypothesis that acute and short-term dietary fat overload will impair the skeletal muscle protein synthetic response to dietary protein ingestion. Eight overweight/obese males [46.4±1.4 years, BMI 32.3±5.4 kg/m2] participated in the acute feeding study, which consisted of 2 randomised crossover trials. On each occasion, subjects ingested an oral meal (with and without fat emulsion) 4h before the coingestion of milk protein, intrinsically labelled with [1-13C]phenylalanine, and dextrose. Nine overweight/obese males [44.0±1.7 years, BMI 30.1±1.1 kg/m2] participated in the chronic study, which consisted of a baseline 1-week isocaloric diet followed by a 2-week high fat diet (+25% energy excess). Acutely, incorporation of dietary amino acids into the skeletal muscle was 2-fold higher (P<0.05) in the lipid trial compared to control. There was no effect of prior lipid ingestion on indices of insulin sensitivity (muscle glucose uptake, PDC activity and Akt phosphorylation) in response to the protein/dextrose drink. Fat overfeeding had no effect on muscle protein synthesis or glucose disposal in response to whey protein ingestion, despite increased muscle DAG C16:0 (P=0.06) and ceramide C16:0 (P<0.01) levels. Neither acute nor short-term dietary fat overload has a detrimental effect on skeletal muscle protein synthetic response to dietary protein ingestion in overweight/obese men, suggesting dietary-induced accumulation of intramuscular lipids per se is not associated with anabolic resistance.

KW - dietary fat

KW - obesity

KW - postprandial period

KW - skeletal muscle protein synthesis

KW - intramuscular lipids

U2 - 10.1152/ajpendo.00344.2019

DO - 10.1152/ajpendo.00344.2019

M3 - Journal article

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 1522-1555

ER -