Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Effect of aluminium on expression and processing of amyloid precursor protein
AU - Neill, D
AU - Leake, A
AU - Hughes, D
AU - Keith, A B
AU - Taylor, G A
AU - Allsop, D
AU - Rima, B K
AU - Morris, C
AU - Candy, J M
AU - Edwardson, J A
PY - 1996/11/15
Y1 - 1996/11/15
N2 - The environmental agent aluminium has been extensively investigated for a potential role in the aetiology of Alzheimer's disease. Despite many investigations there is at present no definite proof for any involvement. If aluminium is involved it is possible that its action is mediated through interaction with the synthesis or processing of amyloid precursor protein (APP). The present study compared aluminium loaded IMR-32 neuroblastoma cells and rat brains with control cells and brains to determine if aluminium affected APP expression and/or processing. In the IMR-32 model system aluminium had no effect on steady-state APP mRNA levels or on the ratio of individual isoforms. It also had no quantitative or qualitative effect on APP-immunoreactive bands detected in protein extracts from conditioned medium of these cells. In total cell extracts, aluminium reduced the intensity of APP-immunoreactive bands between 120-105 kDa but had no effect on a 9 kDa band. In rat brains, aluminium had no effect on APP-immunoreactive bands from soluble or insoluble-membranous extracts. The results, in general, provide no evidence for any effect of aluminium on APP expression or processing.
AB - The environmental agent aluminium has been extensively investigated for a potential role in the aetiology of Alzheimer's disease. Despite many investigations there is at present no definite proof for any involvement. If aluminium is involved it is possible that its action is mediated through interaction with the synthesis or processing of amyloid precursor protein (APP). The present study compared aluminium loaded IMR-32 neuroblastoma cells and rat brains with control cells and brains to determine if aluminium affected APP expression and/or processing. In the IMR-32 model system aluminium had no effect on steady-state APP mRNA levels or on the ratio of individual isoforms. It also had no quantitative or qualitative effect on APP-immunoreactive bands detected in protein extracts from conditioned medium of these cells. In total cell extracts, aluminium reduced the intensity of APP-immunoreactive bands between 120-105 kDa but had no effect on a 9 kDa band. In rat brains, aluminium had no effect on APP-immunoreactive bands from soluble or insoluble-membranous extracts. The results, in general, provide no evidence for any effect of aluminium on APP expression or processing.
KW - Aluminum
KW - Alzheimer Disease
KW - Amyloid beta-Protein Precursor
KW - Animals
KW - Apoproteins
KW - Blotting, Northern
KW - Blotting, Western
KW - Brain Chemistry
KW - Gene Expression Regulation
KW - Humans
KW - Molecular Weight
KW - Nerve Tissue Proteins
KW - Neuroblastoma
KW - Protein Processing, Post-Translational
KW - RNA, Messenger
KW - Rats
KW - Transferrin
KW - Tumor Cells, Cultured
U2 - 10.1002/(SICI)1097-4547(19961115)46:4<395::AID-JNR1>3.0.CO;2-B
DO - 10.1002/(SICI)1097-4547(19961115)46:4<395::AID-JNR1>3.0.CO;2-B
M3 - Journal article
C2 - 8950699
VL - 46
SP - 395
EP - 403
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 4
ER -