Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Effect of calcium hydroxide on mechanical strength and biological properties of bioactive glass
AU - Shah, A.T.
AU - Batool, M.
AU - Chaudhry, A.A.
AU - Iqbal, F.
AU - Javaid, A.
AU - Zahid, S.
AU - Ilyas, K.
AU - bin Qasim, S.
AU - Khan, A.F.
AU - Khan, A.S.
AU - ur Rehman, I.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - In this manuscript for the first time calcium hydroxide (Ca(OH)2) has been used for preparation of bioactive glass (BG-2) by co-precipitation method and compared with glass prepared using calcium nitrate tetrahydrate Ca(NO3)2·4H2O (BG-1), which is a conventional source of calcium. The new source positively affected physical, biological and mechanical properties of BG-2. The glasses were characterized by Fourier transform infrared (FTIR), X-Ray Diffractometer (XRD), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis/Differential Scanning Calorimetry (TGA-DSC), BET surface area analysis and Knoop hardness. The results showed that BG-2 possessed relatively larger surface properties (100 m2 g-1 surface area) as compared to BG-1 (78 m2 g-1), spherical morphology and crystalline phases (wollastonite and apatite) after sintering at lower than conventional temperature. These properties contribute critical role in both mechanical and biological properties of glasses. The Knoop hardness measurements revealed that BG-2 possessed much better hardness (0.43±0.06 GPa at 680 °C and 2.16±0.46 GPa at 980 °C) than BG-1 (0.24±0.01 at 680 °C and 0.57±0.07GPA at 980 °C) under same conditions. Alamar blue Assay and confocal microscopy revealed that BG-2 exhibited better attachment and proliferation of MG63 cells. Based on the improved biological properties of BG-2 as a consequent of novel calcium source selection, BG-2 is proposed as a bioactive ceramic for hard tissue repair and regeneration applications.
AB - In this manuscript for the first time calcium hydroxide (Ca(OH)2) has been used for preparation of bioactive glass (BG-2) by co-precipitation method and compared with glass prepared using calcium nitrate tetrahydrate Ca(NO3)2·4H2O (BG-1), which is a conventional source of calcium. The new source positively affected physical, biological and mechanical properties of BG-2. The glasses were characterized by Fourier transform infrared (FTIR), X-Ray Diffractometer (XRD), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis/Differential Scanning Calorimetry (TGA-DSC), BET surface area analysis and Knoop hardness. The results showed that BG-2 possessed relatively larger surface properties (100 m2 g-1 surface area) as compared to BG-1 (78 m2 g-1), spherical morphology and crystalline phases (wollastonite and apatite) after sintering at lower than conventional temperature. These properties contribute critical role in both mechanical and biological properties of glasses. The Knoop hardness measurements revealed that BG-2 possessed much better hardness (0.43±0.06 GPa at 680 °C and 2.16±0.46 GPa at 980 °C) than BG-1 (0.24±0.01 at 680 °C and 0.57±0.07GPA at 980 °C) under same conditions. Alamar blue Assay and confocal microscopy revealed that BG-2 exhibited better attachment and proliferation of MG63 cells. Based on the improved biological properties of BG-2 as a consequent of novel calcium source selection, BG-2 is proposed as a bioactive ceramic for hard tissue repair and regeneration applications.
KW - Bioactive glass
KW - Cytotoxicity
KW - Hardness
KW - Phase analysis
KW - Structural analysis
KW - Thermal behavior
KW - Biomechanics
KW - Calcium
KW - Fourier transform infrared spectroscopy
KW - Hydrated lime
KW - Phosphate minerals
KW - Precipitation (chemical)
KW - Scanning electron microscopy
KW - Silicate minerals
KW - Sintering
KW - Surface properties
KW - Thermogravimetric analysis
KW - Tissue regeneration
KW - Biological properties
KW - Calcium nitrate tetra-hydrate
KW - Coprecipitation method
KW - Fourier transform infrared
KW - Spherical morphologies
KW - Thermal behaviors
KW - X ray diffractometers
KW - biomaterial
KW - calcium hydroxide
KW - calcium nitrate tetrahydrate
KW - glass
KW - unclassified drug
KW - absorption
KW - Article
KW - biomechanics
KW - comparative study
KW - confocal microscopy
KW - controlled study
KW - decomposition
KW - desorption
KW - differential scanning calorimetry
KW - glass transition temperature
KW - hardness
KW - human
KW - human cell
KW - infrared spectroscopy
KW - osteoblast
KW - osteosarcoma cell
KW - physical chemistry
KW - precipitation
KW - priority journal
KW - scanning electron microscopy
KW - strength
KW - stretching
KW - surface area
KW - surface property
KW - thermogravimetry
KW - tissue engineering
KW - X ray diffraction
KW - ceramics
KW - chemistry
KW - Biocompatible Materials
KW - Calcium Hydroxide
KW - Ceramics
KW - Glass
KW - Microscopy, Electron, Scanning
KW - Spectroscopy, Fourier Transform Infrared
KW - Surface Properties
KW - X-Ray Diffraction
U2 - 10.1016/j.jmbbm.2016.03.030
DO - 10.1016/j.jmbbm.2016.03.030
M3 - Journal article
VL - 61
SP - 617
EP - 626
JO - Journal of the Mechanical Behavior of Biomedical Materials
JF - Journal of the Mechanical Behavior of Biomedical Materials
SN - 1751-6161
ER -