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Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane.

Research output: Contribution to journalJournal article

Published

  • R. F. Fornazier
  • R. R. Ferreira
  • A. P. Vitória
  • S. M. G. Molina
  • P. J. Lea
  • R. A. Azevedo
Journal publication date03/2002
JournalBiologia Plantarum
Journal number1
Volume45
Number of pages7
Pages91-97
Original languageEnglish

Abstract

Sugar cane (Saccharum officinarum L. cv. Copersucar SP80-3280) seedlings were grown in nutrient solution with varying concentrations (0, 2 and 5 mM) of cadmium chloride for 96 h. Leaves were analysed for catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Although a clear effect of CdCl2 on plant growth was observed, the activity of SOD was not altered significantly. However, the CAT activity decreased as the concentration of CdCl2 increased. GR exhibits a significant increase in activity at 2 and 5 mM CdCl2. CAT and SOD isoenzymes were further characterised by analysis in non-denaturing PAGE. Activity staining for SOD revealed up to seven isoenzymes in untreated control and 2 mM CdCl2 treated plants, corresponding to Cu/Zn-SOD isoenzymes. At 5 mM CdCl2, only six Cu/Zn-SOD isoenzymes were observed. No Fe-SOD and Mn-SOD isoenzymes were detected. For CAT, one band of activity was observed.