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Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane.

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Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane. / Fornazier, R. F.; Ferreira, R. R.; Vitória, A. P. et al.
In: Biologia Plantarum, Vol. 45, No. 1, 03.2002, p. 91-97.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Fornazier, RF, Ferreira, RR, Vitória, AP, Molina, SMG, Lea, PJ & Azevedo, RA 2002, 'Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane.', Biologia Plantarum, vol. 45, no. 1, pp. 91-97. https://doi.org/10.1023/A:1015100624229

APA

Fornazier, R. F., Ferreira, R. R., Vitória, A. P., Molina, S. M. G., Lea, P. J., & Azevedo, R. A. (2002). Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane. Biologia Plantarum, 45(1), 91-97. https://doi.org/10.1023/A:1015100624229

Vancouver

Fornazier RF, Ferreira RR, Vitória AP, Molina SMG, Lea PJ, Azevedo RA. Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane. Biologia Plantarum. 2002 Mar;45(1):91-97. doi: 10.1023/A:1015100624229

Author

Fornazier, R. F. ; Ferreira, R. R. ; Vitória, A. P. et al. / Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane. In: Biologia Plantarum. 2002 ; Vol. 45, No. 1. pp. 91-97.

Bibtex

@article{a8a4ba9c47794b4b9f3b4c2667772e94,
title = "Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane.",
abstract = "Sugar cane (Saccharum officinarum L. cv. Copersucar SP80-3280) seedlings were grown in nutrient solution with varying concentrations (0, 2 and 5 mM) of cadmium chloride for 96 h. Leaves were analysed for catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Although a clear effect of CdCl2 on plant growth was observed, the activity of SOD was not altered significantly. However, the CAT activity decreased as the concentration of CdCl2 increased. GR exhibits a significant increase in activity at 2 and 5 mM CdCl2. CAT and SOD isoenzymes were further characterised by analysis in non-denaturing PAGE. Activity staining for SOD revealed up to seven isoenzymes in untreated control and 2 mM CdCl2 treated plants, corresponding to Cu/Zn-SOD isoenzymes. At 5 mM CdCl2, only six Cu/Zn-SOD isoenzymes were observed. No Fe-SOD and Mn-SOD isoenzymes were detected. For CAT, one band of activity was observed.",
keywords = "catalase - glutathione reductase - heavy metals - phytochelatins - reactive oxygen species - Saccharum officinarum - superoxide dismutase",
author = "Fornazier, {R. F.} and Ferreira, {R. R.} and Vit{\'o}ria, {A. P.} and Molina, {S. M. G.} and Lea, {P. J.} and Azevedo, {R. A.}",
year = "2002",
month = mar,
doi = "10.1023/A:1015100624229",
language = "English",
volume = "45",
pages = "91--97",
journal = "Biologia Plantarum",
issn = "0006-3134",
publisher = "Springer Netherlands",
number = "1",

}

RIS

TY - JOUR

T1 - Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane.

AU - Fornazier, R. F.

AU - Ferreira, R. R.

AU - Vitória, A. P.

AU - Molina, S. M. G.

AU - Lea, P. J.

AU - Azevedo, R. A.

PY - 2002/3

Y1 - 2002/3

N2 - Sugar cane (Saccharum officinarum L. cv. Copersucar SP80-3280) seedlings were grown in nutrient solution with varying concentrations (0, 2 and 5 mM) of cadmium chloride for 96 h. Leaves were analysed for catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Although a clear effect of CdCl2 on plant growth was observed, the activity of SOD was not altered significantly. However, the CAT activity decreased as the concentration of CdCl2 increased. GR exhibits a significant increase in activity at 2 and 5 mM CdCl2. CAT and SOD isoenzymes were further characterised by analysis in non-denaturing PAGE. Activity staining for SOD revealed up to seven isoenzymes in untreated control and 2 mM CdCl2 treated plants, corresponding to Cu/Zn-SOD isoenzymes. At 5 mM CdCl2, only six Cu/Zn-SOD isoenzymes were observed. No Fe-SOD and Mn-SOD isoenzymes were detected. For CAT, one band of activity was observed.

AB - Sugar cane (Saccharum officinarum L. cv. Copersucar SP80-3280) seedlings were grown in nutrient solution with varying concentrations (0, 2 and 5 mM) of cadmium chloride for 96 h. Leaves were analysed for catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Although a clear effect of CdCl2 on plant growth was observed, the activity of SOD was not altered significantly. However, the CAT activity decreased as the concentration of CdCl2 increased. GR exhibits a significant increase in activity at 2 and 5 mM CdCl2. CAT and SOD isoenzymes were further characterised by analysis in non-denaturing PAGE. Activity staining for SOD revealed up to seven isoenzymes in untreated control and 2 mM CdCl2 treated plants, corresponding to Cu/Zn-SOD isoenzymes. At 5 mM CdCl2, only six Cu/Zn-SOD isoenzymes were observed. No Fe-SOD and Mn-SOD isoenzymes were detected. For CAT, one band of activity was observed.

KW - catalase - glutathione reductase - heavy metals - phytochelatins - reactive oxygen species - Saccharum officinarum - superoxide dismutase

U2 - 10.1023/A:1015100624229

DO - 10.1023/A:1015100624229

M3 - Journal article

VL - 45

SP - 91

EP - 97

JO - Biologia Plantarum

JF - Biologia Plantarum

SN - 0006-3134

IS - 1

ER -