Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Electroactive tissue scaffolds with aligned pores as instructive platforms for biomimetic tissue engineering
AU - Hardy, John G.
AU - Cornelison, R. Chase
AU - Sukhavasi, Rushi C.
AU - Saballos, Richard J.
AU - Vu, Philip
AU - Kaplan, David L.
AU - Schmidt, Christine E.
PY - 2015/1/14
Y1 - 2015/1/14
N2 - Tissues in the body are hierarchically structured composite materials with tissue-specific chemical and topographical properties. Here we report the preparation of tissue scaffolds with macroscopic pores generated via the dissolution of a sacrificial supramolecular polymer-based crystal template (urea) from a biodegradable polymer-based scaffold (polycaprolactone, PCL). Furthermore, we report a method of aligning the supramolecular polymer-based crystals within the PCL, and that the dissolution of the sacrificial urea yields scaffolds with macroscopic pores that are aligned over long, clinically-relevant distances (i.e., centimeter scale). The pores act as topographical cues to which rat Schwann cells respond by aligning with the long axis of the pores. Generation of an interpenetrating network of polypyrrole (PPy) and poly(styrene sulfonate) (PSS) in the scaffolds yields electroactive tissue scaffolds that allow the electrical stimulation of Schwann cells cultured on the scaffolds which increases the production of nerve growth factor (NGF).
AB - Tissues in the body are hierarchically structured composite materials with tissue-specific chemical and topographical properties. Here we report the preparation of tissue scaffolds with macroscopic pores generated via the dissolution of a sacrificial supramolecular polymer-based crystal template (urea) from a biodegradable polymer-based scaffold (polycaprolactone, PCL). Furthermore, we report a method of aligning the supramolecular polymer-based crystals within the PCL, and that the dissolution of the sacrificial urea yields scaffolds with macroscopic pores that are aligned over long, clinically-relevant distances (i.e., centimeter scale). The pores act as topographical cues to which rat Schwann cells respond by aligning with the long axis of the pores. Generation of an interpenetrating network of polypyrrole (PPy) and poly(styrene sulfonate) (PSS) in the scaffolds yields electroactive tissue scaffolds that allow the electrical stimulation of Schwann cells cultured on the scaffolds which increases the production of nerve growth factor (NGF).
KW - BIOMATERIALS
KW - POROSITY
KW - tissue scaffold
KW - tissue engineering
KW - Regenerative Medicine
KW - Chemistry(all)
KW - Biomaterials
KW - electroactive polymers
KW - microfabrication
KW - nerve guide
KW - peripheral nerve
KW - plastic electronics
KW - topography
U2 - 10.3390/bioengineering2010015
DO - 10.3390/bioengineering2010015
M3 - Journal article
VL - 2
SP - 15
EP - 34
JO - Bioengineering
JF - Bioengineering
SN - 2306-5354
IS - 1
ER -