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Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius.

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Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius. / Tinney, Glenda W.; Pritchard, Susan C.; Gonzalez, Raquel et al.
In: Plant Molecular Biology Reporter, Vol. 20, No. 3, 09.2002, p. 309-310.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Tinney, GW, Pritchard, SC, Gonzalez, R, Paul, ND, Hatcher, PE & Taylor, JE 2002, 'Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius.', Plant Molecular Biology Reporter, vol. 20, no. 3, pp. 309-310. https://doi.org/10.1007/BF02782470

APA

Tinney, G. W., Pritchard, S. C., Gonzalez, R., Paul, N. D., Hatcher, P. E., & Taylor, J. E. (2002). Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius. Plant Molecular Biology Reporter, 20(3), 309-310. https://doi.org/10.1007/BF02782470

Vancouver

Tinney GW, Pritchard SC, Gonzalez R, Paul ND, Hatcher PE, Taylor JE. Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius. Plant Molecular Biology Reporter. 2002 Sept;20(3):309-310. doi: 10.1007/BF02782470

Author

Tinney, Glenda W. ; Pritchard, Susan C. ; Gonzalez, Raquel et al. / Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius. In: Plant Molecular Biology Reporter. 2002 ; Vol. 20, No. 3. pp. 309-310.

Bibtex

@article{0d96c23cbcc3490c93cc7db7eadb65b5,
title = "Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius.",
abstract = "Many plant RNA isolation techniques aim to prevent contamination by means of secondary phenolics, carbohydrates, RNase, and other chemicals. However, when applied in our laboratory to the isolation of RNA fromRumex obtusifolius, these protocols failed to produce good quality RNA. A major problem was contamination of the RNA samples with the secondary metabolite oxalate. The relative quantities of guanidine isothiocyanate extraction buffer to plant tissue used in the protocol had significant effects on oxalate contamination. An increase in extraction buffer, from 1.5 mL in the original method to 15 mL per 200–300 mg of tissue in our protocol, removed the oxalate from the RNA. This RNA was of a good quality and was suitable for molecular biology applications.",
keywords = "RNA extraction - oxalate contamination - Rumex",
author = "Tinney, {Glenda W.} and Pritchard, {Susan C.} and Raquel Gonzalez and Paul, {Nigel D.} and Hatcher, {Paul E.} and Taylor, {Jane E.}",
year = "2002",
month = sep,
doi = "10.1007/BF02782470",
language = "English",
volume = "20",
pages = "309--310",
journal = "Plant Molecular Biology Reporter",
issn = "0735-9640",
publisher = "Springer New York",
number = "3",

}

RIS

TY - JOUR

T1 - Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius.

AU - Tinney, Glenda W.

AU - Pritchard, Susan C.

AU - Gonzalez, Raquel

AU - Paul, Nigel D.

AU - Hatcher, Paul E.

AU - Taylor, Jane E.

PY - 2002/9

Y1 - 2002/9

N2 - Many plant RNA isolation techniques aim to prevent contamination by means of secondary phenolics, carbohydrates, RNase, and other chemicals. However, when applied in our laboratory to the isolation of RNA fromRumex obtusifolius, these protocols failed to produce good quality RNA. A major problem was contamination of the RNA samples with the secondary metabolite oxalate. The relative quantities of guanidine isothiocyanate extraction buffer to plant tissue used in the protocol had significant effects on oxalate contamination. An increase in extraction buffer, from 1.5 mL in the original method to 15 mL per 200–300 mg of tissue in our protocol, removed the oxalate from the RNA. This RNA was of a good quality and was suitable for molecular biology applications.

AB - Many plant RNA isolation techniques aim to prevent contamination by means of secondary phenolics, carbohydrates, RNase, and other chemicals. However, when applied in our laboratory to the isolation of RNA fromRumex obtusifolius, these protocols failed to produce good quality RNA. A major problem was contamination of the RNA samples with the secondary metabolite oxalate. The relative quantities of guanidine isothiocyanate extraction buffer to plant tissue used in the protocol had significant effects on oxalate contamination. An increase in extraction buffer, from 1.5 mL in the original method to 15 mL per 200–300 mg of tissue in our protocol, removed the oxalate from the RNA. This RNA was of a good quality and was suitable for molecular biology applications.

KW - RNA extraction - oxalate contamination - Rumex

U2 - 10.1007/BF02782470

DO - 10.1007/BF02782470

M3 - Journal article

VL - 20

SP - 309

EP - 310

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

SN - 0735-9640

IS - 3

ER -