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Ethylene metabolism in Pisum sativum L. : Kinetic parameters, the effects of propylene, silver and carbon dioxide and comparison with other systems

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Ethylene metabolism in Pisum sativum L. : Kinetic parameters, the effects of propylene, silver and carbon dioxide and comparison with other systems. / Evans, David E; Smith, Aileen R; Taylor, Jane; Halo, Michael A.

In: Plant Growth Regulation, Vol. 2, No. 3, 09.1984, p. 187-195.

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Evans, David E ; Smith, Aileen R ; Taylor, Jane ; Halo, Michael A. / Ethylene metabolism in Pisum sativum L. : Kinetic parameters, the effects of propylene, silver and carbon dioxide and comparison with other systems. In: Plant Growth Regulation. 1984 ; Vol. 2, No. 3. pp. 187-195.

Bibtex

@article{a1442a032a57472fbbce7288271e7801,
title = "Ethylene metabolism in Pisum sativum L. : Kinetic parameters, the effects of propylene, silver and carbon dioxide and comparison with other systems",
abstract = "The kinetic parameters of in vivo ethylene metabolism by seedlings of Pisum sativum L. cv. Alaska have been determined. The oxidation of ethylene to CO2, (Ox) and the incorporation of ethylene into the tissue (TI) were both shown to display Michaelis-Menten kinetics (Km Ox = 0.9 × 10–6 M liquid phase, Vmax Ox = 2.4 × 10–10 moles g– dry mass h–1 Km TI = 1.6 × 10–6 M liquid phase, Vmax TI = 4.5 × 10–10 moles g–1 dry mass h–1). Propylene competitively inhibited both Ox (Ki = 7.0 × 10–6 M) and TI (Ki = 3.7 × 10–7 M). A system comparable to Ox was absent from imbibed cotyledons of Vicia faba L. cv. Aquadulce even at saturating concentrations of ethylene similar to those used in kinetic analysis on Pisum. Silver ions were shown to inhibit TI but promoted Ox, while carbon dioxide inhibited Ox but promoted TI. Kinetic data on both these effects are presented. Data on the effect of a range of concentrations of CO2 on TI and Ox are also presented.",
keywords = "Propylene, Ethylene metabolism , Silver , Carbon dioxide , Pisum sativum",
author = "Evans, {David E} and Smith, {Aileen R} and Jane Taylor and Halo, {Michael A}",
year = "1984",
month = sep
doi = "10.1007/BF00124767",
language = "English",
volume = "2",
pages = "187--195",
journal = "Plant Growth Regulation",
issn = "0167-6903",
publisher = "Springer Netherlands",
number = "3",

}

RIS

TY - JOUR

T1 - Ethylene metabolism in Pisum sativum L. : Kinetic parameters, the effects of propylene, silver and carbon dioxide and comparison with other systems

AU - Evans, David E

AU - Smith, Aileen R

AU - Taylor, Jane

AU - Halo, Michael A

PY - 1984/9

Y1 - 1984/9

N2 - The kinetic parameters of in vivo ethylene metabolism by seedlings of Pisum sativum L. cv. Alaska have been determined. The oxidation of ethylene to CO2, (Ox) and the incorporation of ethylene into the tissue (TI) were both shown to display Michaelis-Menten kinetics (Km Ox = 0.9 × 10–6 M liquid phase, Vmax Ox = 2.4 × 10–10 moles g– dry mass h–1 Km TI = 1.6 × 10–6 M liquid phase, Vmax TI = 4.5 × 10–10 moles g–1 dry mass h–1). Propylene competitively inhibited both Ox (Ki = 7.0 × 10–6 M) and TI (Ki = 3.7 × 10–7 M). A system comparable to Ox was absent from imbibed cotyledons of Vicia faba L. cv. Aquadulce even at saturating concentrations of ethylene similar to those used in kinetic analysis on Pisum. Silver ions were shown to inhibit TI but promoted Ox, while carbon dioxide inhibited Ox but promoted TI. Kinetic data on both these effects are presented. Data on the effect of a range of concentrations of CO2 on TI and Ox are also presented.

AB - The kinetic parameters of in vivo ethylene metabolism by seedlings of Pisum sativum L. cv. Alaska have been determined. The oxidation of ethylene to CO2, (Ox) and the incorporation of ethylene into the tissue (TI) were both shown to display Michaelis-Menten kinetics (Km Ox = 0.9 × 10–6 M liquid phase, Vmax Ox = 2.4 × 10–10 moles g– dry mass h–1 Km TI = 1.6 × 10–6 M liquid phase, Vmax TI = 4.5 × 10–10 moles g–1 dry mass h–1). Propylene competitively inhibited both Ox (Ki = 7.0 × 10–6 M) and TI (Ki = 3.7 × 10–7 M). A system comparable to Ox was absent from imbibed cotyledons of Vicia faba L. cv. Aquadulce even at saturating concentrations of ethylene similar to those used in kinetic analysis on Pisum. Silver ions were shown to inhibit TI but promoted Ox, while carbon dioxide inhibited Ox but promoted TI. Kinetic data on both these effects are presented. Data on the effect of a range of concentrations of CO2 on TI and Ox are also presented.

KW - Propylene

KW - Ethylene metabolism

KW - Silver

KW - Carbon dioxide

KW - Pisum sativum

U2 - 10.1007/BF00124767

DO - 10.1007/BF00124767

M3 - Journal article

VL - 2

SP - 187

EP - 195

JO - Plant Growth Regulation

JF - Plant Growth Regulation

SN - 0167-6903

IS - 3

ER -