Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Evaluation of a temperate climate mosquito, Ochlerotatus detritus (=Aedes detritus), as a potential vector of Japanese encephalitis virus
AU - Mackenzie-Impoinvil, L.
AU - Impoinvil, D. E.
AU - Dillon, R. J.
AU - Ranson, H.
AU - Fooks, A. R.
AU - Solomon, T.
PY - 2015/3
Y1 - 2015/3
N2 - The U.K. has not yet experienced a confirmed outbreak of mosquito-borne virus transmission to people or livestock despite numerous autochthonous epizootic and human outbreaks of mosquito-borne diseases on the European mainland. Indeed, whether or not British mosquitoes are competent to transmit arboviruses has not been established. Therefore, the competence of a local (temperate) British mosquito species, Ochlerotatus detritus (=Aedes detritus) (Diptera: Culicidae) for transmission of a member of the genus Flavivirus, Japanese encephalitis virus (JEV) as a model for mosquito-borne virus transmission was assessed. The JEV competence in a laboratory strain of Culex quinquefasciatus (Diptera: Culicidae), a previously incriminated JEV vector, was also evaluated as a positive control. Ochlerotatus detritus adults were reared from field-collected juvenile stages. In oral infection bioassays, adult females developed disseminated infections and were able to transmit virus as determined by the isolation of virus in saliva secretions. When pooled at 7-21days post-infection, 13% and 25% of O.detritus were able to transmit JEV when held at 23 degrees C and 28 degrees C, respectively. Similar results were obtained for C.quinquefasciatus. To our knowledge, this study is the first to demonstrate that a British mosquito species, O.detritus, is a potential vector of an exotic flavivirus.
AB - The U.K. has not yet experienced a confirmed outbreak of mosquito-borne virus transmission to people or livestock despite numerous autochthonous epizootic and human outbreaks of mosquito-borne diseases on the European mainland. Indeed, whether or not British mosquitoes are competent to transmit arboviruses has not been established. Therefore, the competence of a local (temperate) British mosquito species, Ochlerotatus detritus (=Aedes detritus) (Diptera: Culicidae) for transmission of a member of the genus Flavivirus, Japanese encephalitis virus (JEV) as a model for mosquito-borne virus transmission was assessed. The JEV competence in a laboratory strain of Culex quinquefasciatus (Diptera: Culicidae), a previously incriminated JEV vector, was also evaluated as a positive control. Ochlerotatus detritus adults were reared from field-collected juvenile stages. In oral infection bioassays, adult females developed disseminated infections and were able to transmit virus as determined by the isolation of virus in saliva secretions. When pooled at 7-21days post-infection, 13% and 25% of O.detritus were able to transmit JEV when held at 23 degrees C and 28 degrees C, respectively. Similar results were obtained for C.quinquefasciatus. To our knowledge, this study is the first to demonstrate that a British mosquito species, O.detritus, is a potential vector of an exotic flavivirus.
KW - Ochlerotatus detritus
KW - British mosquito
KW - Japanese encephalitis virus
KW - vector competence
KW - WEST-NILE-VIRUS
KW - CULEX-MODESTUS
KW - SINDBIS-VIRUS
KW - USUTU-VIRUS
KW - FEVER VIRUS
KW - INFECTION
KW - TRANSMISSION
KW - CULICIDAE
KW - DIPTERA
KW - SALIVA
U2 - 10.1111/mve.12083
DO - 10.1111/mve.12083
M3 - Journal article
VL - 29
SP - 1
EP - 9
JO - Medical and Veterinary Entomology
JF - Medical and Veterinary Entomology
SN - 0269-283X
IS - 1
ER -