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Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis. / Lim, E K ; Baldauf, S ; Li, Y et al.
In: Glycobiology, Vol. 13, No. 3, 2002, p. 139-145.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Lim, EK, Baldauf, S, Li, Y, Elias, L, Worrall, D, Spencer, SP, Jackson, RG, Taguchi, G, Ross, J & Bowles, DJ 2002, 'Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis', Glycobiology, vol. 13, no. 3, pp. 139-145. https://doi.org/10.1093/glycob/cwg017

APA

Lim, E. K., Baldauf, S., Li, Y., Elias, L., Worrall, D., Spencer, S. P., Jackson, R. G., Taguchi, G., Ross, J., & Bowles, D. J. (2002). Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis. Glycobiology, 13(3), 139-145. https://doi.org/10.1093/glycob/cwg017

Vancouver

Lim EK, Baldauf S, Li Y, Elias L, Worrall D, Spencer SP et al. Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis. Glycobiology. 2002;13(3):139-145. doi: 10.1093/glycob/cwg017

Author

Lim, E K ; Baldauf, S ; Li, Y et al. / Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis. In: Glycobiology. 2002 ; Vol. 13, No. 3. pp. 139-145.

Bibtex

@article{ae70af4f37ac4c3daa61cb0eed7ff27e,
title = "Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis",
abstract = "The complete sequence of the Arabidopsis genome enables definitive characterization of multigene families and analysis of their phylogenetic relationships. Using a consensus sequence previously defined for glycosyltransferases that use small-molecular-weight acceptors, 107 gene sequences were identified in the Arabidopsis genome and used to construct a phylogenetic tree. Screening recombinant proteins for their catalytic activities in vitro has revealed enzymes active toward physiologically important substrates, including hormones and secondary metabolites. The aim of this study has been to use the phylogenetic relationships across the entire family to explore the evolution of substrate recognition and regioselectivity of glucosylation. Hydroxycoumarins have been used as the model substrates for the analysis in which 90 sequences have been assayed and 48 sequences shown to recognize these compounds. The study has revealed activity in 6 of the 14 phylogenetic groups of the multigene family, suggesting that basic features of substrate recognition are retained across substantial evolutionary periods.",
keywords = "Arabidopsis, glucosyltransferases, hydroxycoumarins, phylogeny, plant secondary metabolites, BIOCHEMICAL-CHARACTERIZATION, DRAFT SEQUENCE, UDP-GLUCOSE, GLUCOSYLTRANSFERASE, PLANT, METABOLISM, ACID, IDENTIFICATION, SCOPOLETIN",
author = "Lim, {E K} and S Baldauf and Y Li and L Elias and D Worrall and Spencer, {S P} and Jackson, {R G} and G Taguchi and J Ross and Bowles, {D J}",
year = "2002",
doi = "10.1093/glycob/cwg017",
language = "English",
volume = "13",
pages = "139--145",
journal = "Glycobiology",
issn = "1460-2423",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis

AU - Lim, E K

AU - Baldauf, S

AU - Li, Y

AU - Elias, L

AU - Worrall, D

AU - Spencer, S P

AU - Jackson, R G

AU - Taguchi, G

AU - Ross, J

AU - Bowles, D J

PY - 2002

Y1 - 2002

N2 - The complete sequence of the Arabidopsis genome enables definitive characterization of multigene families and analysis of their phylogenetic relationships. Using a consensus sequence previously defined for glycosyltransferases that use small-molecular-weight acceptors, 107 gene sequences were identified in the Arabidopsis genome and used to construct a phylogenetic tree. Screening recombinant proteins for their catalytic activities in vitro has revealed enzymes active toward physiologically important substrates, including hormones and secondary metabolites. The aim of this study has been to use the phylogenetic relationships across the entire family to explore the evolution of substrate recognition and regioselectivity of glucosylation. Hydroxycoumarins have been used as the model substrates for the analysis in which 90 sequences have been assayed and 48 sequences shown to recognize these compounds. The study has revealed activity in 6 of the 14 phylogenetic groups of the multigene family, suggesting that basic features of substrate recognition are retained across substantial evolutionary periods.

AB - The complete sequence of the Arabidopsis genome enables definitive characterization of multigene families and analysis of their phylogenetic relationships. Using a consensus sequence previously defined for glycosyltransferases that use small-molecular-weight acceptors, 107 gene sequences were identified in the Arabidopsis genome and used to construct a phylogenetic tree. Screening recombinant proteins for their catalytic activities in vitro has revealed enzymes active toward physiologically important substrates, including hormones and secondary metabolites. The aim of this study has been to use the phylogenetic relationships across the entire family to explore the evolution of substrate recognition and regioselectivity of glucosylation. Hydroxycoumarins have been used as the model substrates for the analysis in which 90 sequences have been assayed and 48 sequences shown to recognize these compounds. The study has revealed activity in 6 of the 14 phylogenetic groups of the multigene family, suggesting that basic features of substrate recognition are retained across substantial evolutionary periods.

KW - Arabidopsis

KW - glucosyltransferases

KW - hydroxycoumarins

KW - phylogeny

KW - plant secondary metabolites

KW - BIOCHEMICAL-CHARACTERIZATION

KW - DRAFT SEQUENCE

KW - UDP-GLUCOSE

KW - GLUCOSYLTRANSFERASE

KW - PLANT

KW - METABOLISM

KW - ACID

KW - IDENTIFICATION

KW - SCOPOLETIN

U2 - 10.1093/glycob/cwg017

DO - 10.1093/glycob/cwg017

M3 - Journal article

VL - 13

SP - 139

EP - 145

JO - Glycobiology

JF - Glycobiology

SN - 1460-2423

IS - 3

ER -