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Excision of the maize transposable element Ac in flax callus

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Excision of the maize transposable element Ac in flax callus. / Roberts, Michael; Kumar, Amar; Scott, Rod et al.
In: Plant Cell Reports, Vol. 9, No. 7, 11.1990, p. 406-409.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Roberts, M, Kumar, A, Scott, R & Draper, J 1990, 'Excision of the maize transposable element Ac in flax callus', Plant Cell Reports, vol. 9, no. 7, pp. 406-409. https://doi.org/10.1007/BF00232410

APA

Roberts, M., Kumar, A., Scott, R., & Draper, J. (1990). Excision of the maize transposable element Ac in flax callus. Plant Cell Reports, 9(7), 406-409. https://doi.org/10.1007/BF00232410

Vancouver

Roberts M, Kumar A, Scott R, Draper J. Excision of the maize transposable element Ac in flax callus. Plant Cell Reports. 1990 Nov;9(7):406-409. doi: 10.1007/BF00232410

Author

Roberts, Michael ; Kumar, Amar ; Scott, Rod et al. / Excision of the maize transposable element Ac in flax callus. In: Plant Cell Reports. 1990 ; Vol. 9, No. 7. pp. 406-409.

Bibtex

@article{400b8051c9c64c36bce74f6fec9a81e0,
title = "Excision of the maize transposable element Ac in flax callus",
abstract = "The frequency and fidelity of Ac transposition, and that of its non-autonomous derivative Ds, were investigated in flax callus. Flax (Linum usitatissimum var. Antares) hypocotyls were transformed with Agrobacterium Ti plasmid vectors containing the Ac or Ds element inserted within the untranslated leader sequence of a chimaeric neomycin phosphotransferase II gene. Kanamycin resistant tissues were produced as a result of excision of Ac in around 35% of the total number of Ac-containing transformants. In contrast, no excision was observed from transformants containing the Ds element. Whilst Ac appears to have excised completely from T-DNAs, little evidence was found to infer reintegration of the Ac element into the genome.",
keywords = "flax, transposon",
author = "Michael Roberts and Amar Kumar and Rod Scott and John Draper",
year = "1990",
month = nov,
doi = "10.1007/BF00232410",
language = "English",
volume = "9",
pages = "406--409",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "7",

}

RIS

TY - JOUR

T1 - Excision of the maize transposable element Ac in flax callus

AU - Roberts, Michael

AU - Kumar, Amar

AU - Scott, Rod

AU - Draper, John

PY - 1990/11

Y1 - 1990/11

N2 - The frequency and fidelity of Ac transposition, and that of its non-autonomous derivative Ds, were investigated in flax callus. Flax (Linum usitatissimum var. Antares) hypocotyls were transformed with Agrobacterium Ti plasmid vectors containing the Ac or Ds element inserted within the untranslated leader sequence of a chimaeric neomycin phosphotransferase II gene. Kanamycin resistant tissues were produced as a result of excision of Ac in around 35% of the total number of Ac-containing transformants. In contrast, no excision was observed from transformants containing the Ds element. Whilst Ac appears to have excised completely from T-DNAs, little evidence was found to infer reintegration of the Ac element into the genome.

AB - The frequency and fidelity of Ac transposition, and that of its non-autonomous derivative Ds, were investigated in flax callus. Flax (Linum usitatissimum var. Antares) hypocotyls were transformed with Agrobacterium Ti plasmid vectors containing the Ac or Ds element inserted within the untranslated leader sequence of a chimaeric neomycin phosphotransferase II gene. Kanamycin resistant tissues were produced as a result of excision of Ac in around 35% of the total number of Ac-containing transformants. In contrast, no excision was observed from transformants containing the Ds element. Whilst Ac appears to have excised completely from T-DNAs, little evidence was found to infer reintegration of the Ac element into the genome.

KW - flax

KW - transposon

U2 - 10.1007/BF00232410

DO - 10.1007/BF00232410

M3 - Journal article

VL - 9

SP - 406

EP - 409

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 7

ER -