TY - JOUR

T1 - Expanding knowledge of the Rubisco kinetics variability in plant species

T2 - environmental and evolutionary trends

AU - Galmés, Jeroni

AU - Kapralov, Maxim V.

AU - Andralojc, P. John

AU - Conesa, Miquel À.

AU - Keys, Alfred J.

AU - Parry, Martin A. J.

AU - Flexas, Jaume

PY - 2014/9

Y1 - 2014/9

N2 - The present study characterizes the kinetic properties of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from 28 terrestrial plant species, representing different phylogenetic lineages, environmental adaptations and photosynthetic mechanisms. Our findings confirm that past atmospheric CO2/O2 ratio changes and present environmental pressures have influenced Rubisco kinetics. One evolutionary adaptation to a decreasing atmospheric CO2/O2 ratio has been an increase in the affinity of Rubisco for CO2 (Kc falling), and a consequent decrease in the velocity of carboxylation (kcat c), which in turn has been ameliorated by an increase in the proportion of leaf protein accounted by Rubisco. The trade-off between Kc and kcat c was not universal among the species studied and deviations from this relationship occur in extant forms of Rubisco. In species adapted to particular environments, including carnivorous plants, crassulacean acid metabolism species and C3 plants from aquatic and arid habitats, Rubisco has evolved towards increased efficiency, as demonstrated by a higher kcat c/Kc ratio. This variability in kinetics was related to the amino acid sequence of the Rubisco large subunit. Phylogenetic analysis identified 13 residues under positive selection during evolution towards specific Rubisco kinetic parameters. This crucial information provides candidate amino acid replacements, which could be implemented to optimize crop photosynthesis under a range of environmental conditions.

AB - The present study characterizes the kinetic properties of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from 28 terrestrial plant species, representing different phylogenetic lineages, environmental adaptations and photosynthetic mechanisms. Our findings confirm that past atmospheric CO2/O2 ratio changes and present environmental pressures have influenced Rubisco kinetics. One evolutionary adaptation to a decreasing atmospheric CO2/O2 ratio has been an increase in the affinity of Rubisco for CO2 (Kc falling), and a consequent decrease in the velocity of carboxylation (kcat c), which in turn has been ameliorated by an increase in the proportion of leaf protein accounted by Rubisco. The trade-off between Kc and kcat c was not universal among the species studied and deviations from this relationship occur in extant forms of Rubisco. In species adapted to particular environments, including carnivorous plants, crassulacean acid metabolism species and C3 plants from aquatic and arid habitats, Rubisco has evolved towards increased efficiency, as demonstrated by a higher kcat c/Kc ratio. This variability in kinetics was related to the amino acid sequence of the Rubisco large subunit. Phylogenetic analysis identified 13 residues under positive selection during evolution towards specific Rubisco kinetic parameters. This crucial information provides candidate amino acid replacements, which could be implemented to optimize crop photosynthesis under a range of environmental conditions.

KW - Evolution

KW - Photosynthesis

KW - Stress

U2 - 10.1111/pce.12335

DO - 10.1111/pce.12335

M3 - Journal article

C2 - 24689692

AN - SCOPUS:84903974516

VL - 37

SP - 1989

EP - 2001

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 9

ER -