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Exposure to arsenic via drinking water induces 5-hydroxymethylcytosine alteration in rat

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Exposure to arsenic via drinking water induces 5-hydroxymethylcytosine alteration in rat. / Zhang, Jie; Mu, Xiaoli; Xu, Weipan et al.
In: Science of the Total Environment, Vol. 497-498, 01.11.2014, p. 618-625.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Zhang, J, Mu, X, Xu, W, Martin, FL, Alamdar, A, Liu, L, Tian, M, Huang, Q & Shen, H 2014, 'Exposure to arsenic via drinking water induces 5-hydroxymethylcytosine alteration in rat', Science of the Total Environment, vol. 497-498, pp. 618-625. https://doi.org/10.1016/j.scitotenv.2014.08.009

APA

Zhang, J., Mu, X., Xu, W., Martin, F. L., Alamdar, A., Liu, L., Tian, M., Huang, Q., & Shen, H. (2014). Exposure to arsenic via drinking water induces 5-hydroxymethylcytosine alteration in rat. Science of the Total Environment, 497-498, 618-625. https://doi.org/10.1016/j.scitotenv.2014.08.009

Vancouver

Zhang J, Mu X, Xu W, Martin FL, Alamdar A, Liu L et al. Exposure to arsenic via drinking water induces 5-hydroxymethylcytosine alteration in rat. Science of the Total Environment. 2014 Nov 1;497-498:618-625. Epub 2014 Aug 27. doi: 10.1016/j.scitotenv.2014.08.009

Author

Zhang, Jie ; Mu, Xiaoli ; Xu, Weipan et al. / Exposure to arsenic via drinking water induces 5-hydroxymethylcytosine alteration in rat. In: Science of the Total Environment. 2014 ; Vol. 497-498. pp. 618-625.

Bibtex

@article{9ddffb620549466eb969c3d0a6a55148,
title = "Exposure to arsenic via drinking water induces 5-hydroxymethylcytosine alteration in rat",
abstract = "Arsenic exposure has been implicated to alter DNA methylation process in vitro and in vivo, but it remains obscure whether it disrupts DNA demethylation process, which is pivotal for epigenetic regulation. The objective of this descriptive study was to investigate the relationship between arsenic exposure and 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) alterations in various organs. In this study, we exposed male Sprague–Dawley rats to sodium arsenite (0.5, 2 or 10 ppm) via drinking water for 8 weeks. Spleen accumulated 2- to 3-fold higher arsenic levels than liver and heart. Lower arsenic levels were observed in the kidney, pancreas and lung. No significant arsenic-induced global 5mC alterations were observed in the majority of investigated organs. However, arsenic induced organ-specific alterations of 5hmC and/or 5hmC/5mC in some investigated organs, i.e. lung, heart, kidney, pancreas and spleen. Our observations suggest that 5hmC is a more sensitive biomarker of arsenic-induced impacts on epigenetic processes than 5mC. Moreover, demethylation via hydroxylation of 5mC appears to play a central role in the toxic mechanism of arsenic.",
keywords = "Arsenic, DNA methylation, DNA demethylation, 5-Hydroxymethylcytosine, 5-Methylcytosine, Organ specificity",
author = "Jie Zhang and Xiaoli Mu and Weipan Xu and Martin, {Francis Luke} and Ambreen Alamdar and Liangpo Liu and Meiping Tian and Qingyu Huang and Heqing Shen",
year = "2014",
month = nov,
day = "1",
doi = "10.1016/j.scitotenv.2014.08.009",
language = "English",
volume = "497-498",
pages = "618--625",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier Science B.V.",

}

RIS

TY - JOUR

T1 - Exposure to arsenic via drinking water induces 5-hydroxymethylcytosine alteration in rat

AU - Zhang, Jie

AU - Mu, Xiaoli

AU - Xu, Weipan

AU - Martin, Francis Luke

AU - Alamdar, Ambreen

AU - Liu, Liangpo

AU - Tian, Meiping

AU - Huang, Qingyu

AU - Shen, Heqing

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Arsenic exposure has been implicated to alter DNA methylation process in vitro and in vivo, but it remains obscure whether it disrupts DNA demethylation process, which is pivotal for epigenetic regulation. The objective of this descriptive study was to investigate the relationship between arsenic exposure and 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) alterations in various organs. In this study, we exposed male Sprague–Dawley rats to sodium arsenite (0.5, 2 or 10 ppm) via drinking water for 8 weeks. Spleen accumulated 2- to 3-fold higher arsenic levels than liver and heart. Lower arsenic levels were observed in the kidney, pancreas and lung. No significant arsenic-induced global 5mC alterations were observed in the majority of investigated organs. However, arsenic induced organ-specific alterations of 5hmC and/or 5hmC/5mC in some investigated organs, i.e. lung, heart, kidney, pancreas and spleen. Our observations suggest that 5hmC is a more sensitive biomarker of arsenic-induced impacts on epigenetic processes than 5mC. Moreover, demethylation via hydroxylation of 5mC appears to play a central role in the toxic mechanism of arsenic.

AB - Arsenic exposure has been implicated to alter DNA methylation process in vitro and in vivo, but it remains obscure whether it disrupts DNA demethylation process, which is pivotal for epigenetic regulation. The objective of this descriptive study was to investigate the relationship between arsenic exposure and 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) alterations in various organs. In this study, we exposed male Sprague–Dawley rats to sodium arsenite (0.5, 2 or 10 ppm) via drinking water for 8 weeks. Spleen accumulated 2- to 3-fold higher arsenic levels than liver and heart. Lower arsenic levels were observed in the kidney, pancreas and lung. No significant arsenic-induced global 5mC alterations were observed in the majority of investigated organs. However, arsenic induced organ-specific alterations of 5hmC and/or 5hmC/5mC in some investigated organs, i.e. lung, heart, kidney, pancreas and spleen. Our observations suggest that 5hmC is a more sensitive biomarker of arsenic-induced impacts on epigenetic processes than 5mC. Moreover, demethylation via hydroxylation of 5mC appears to play a central role in the toxic mechanism of arsenic.

KW - Arsenic

KW - DNA methylation

KW - DNA demethylation

KW - 5-Hydroxymethylcytosine

KW - 5-Methylcytosine

KW - Organ specificity

U2 - 10.1016/j.scitotenv.2014.08.009

DO - 10.1016/j.scitotenv.2014.08.009

M3 - Journal article

VL - 497-498

SP - 618

EP - 625

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

ER -