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Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana

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Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana. / Gao, Yongshun; Badejo, Adebanjo; Shibata, Hitoshi et al.
In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 9, 05.2011, p. 1783-1788.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Gao, Y, Badejo, A, Shibata, H, Sawa, Y, Maruta, T, Shigeoka, S, Page, M, Smirnoff, N & Ishikawa, T 2011, 'Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana', Bioscience, Biotechnology and Biochemistry, vol. 75, no. 9, pp. 1783-1788. https://doi.org/10.1271/bbb.110320

APA

Gao, Y., Badejo, A., Shibata, H., Sawa, Y., Maruta, T., Shigeoka, S., Page, M., Smirnoff, N., & Ishikawa, T. (2011). Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana. Bioscience, Biotechnology and Biochemistry, 75(9), 1783-1788. https://doi.org/10.1271/bbb.110320

Vancouver

Gao Y, Badejo A, Shibata H, Sawa Y, Maruta T, Shigeoka S et al. Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana. Bioscience, Biotechnology and Biochemistry. 2011 May;75(9):1783-1788. doi: 10.1271/bbb.110320

Author

Gao, Yongshun ; Badejo, Adebanjo ; Shibata, Hitoshi et al. / Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana. In: Bioscience, Biotechnology and Biochemistry. 2011 ; Vol. 75, No. 9. pp. 1783-1788.

Bibtex

@article{740ee653741e429d99ab8f4bb42f6f5c,
title = "Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana",
abstract = "Arabidopsis thaliana contains two GDP-L-galactose phosphorylase genes, VTC2 and VTC5, which are critical for ascorbate (AsA) biosynthesis. We investigated the expression levels of both VTC2 and VTC5 genes in wild-type A. thaliana and the AsA deficient mutants during early seedling growth. Ascorbate accumulated to an equal extent in all genotypes up to 5 d post-germination (DPG). The transcript level of VTC2 was dominant, and increased in parallel with AsA accumulation in the wild type. On the other hand, the expression of VTC5 compensated for the reduced VTC2 transcription levels in the AsA deficient mutant vtc2-1 in young seedlings. A luciferase activity assay indicated that the VTC5 promoter was more active in young (2 DPG) cotyledons and that the VTC2 and VTC5 promoters drove a day-to-night variation in expression. The present work provides clues to the precise roles of VTC2 and VTC5 in AsA biosynthesis in A. thaliana at the young seedling stage.",
author = "Yongshun Gao and Adebanjo Badejo and Hitoshi Shibata and Yoshihiro Sawa and Takanori Maruta and Shigeru Shigeoka and Michael Page and Nicholas Smirnoff and Takahiro Ishikawa",
year = "2011",
month = may,
doi = "10.1271/bbb.110320",
language = "English",
volume = "75",
pages = "1783--1788",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "1347-6947",
publisher = "Taylor & Francis",
number = "9",

}

RIS

TY - JOUR

T1 - Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana

AU - Gao, Yongshun

AU - Badejo, Adebanjo

AU - Shibata, Hitoshi

AU - Sawa, Yoshihiro

AU - Maruta, Takanori

AU - Shigeoka, Shigeru

AU - Page, Michael

AU - Smirnoff, Nicholas

AU - Ishikawa, Takahiro

PY - 2011/5

Y1 - 2011/5

N2 - Arabidopsis thaliana contains two GDP-L-galactose phosphorylase genes, VTC2 and VTC5, which are critical for ascorbate (AsA) biosynthesis. We investigated the expression levels of both VTC2 and VTC5 genes in wild-type A. thaliana and the AsA deficient mutants during early seedling growth. Ascorbate accumulated to an equal extent in all genotypes up to 5 d post-germination (DPG). The transcript level of VTC2 was dominant, and increased in parallel with AsA accumulation in the wild type. On the other hand, the expression of VTC5 compensated for the reduced VTC2 transcription levels in the AsA deficient mutant vtc2-1 in young seedlings. A luciferase activity assay indicated that the VTC5 promoter was more active in young (2 DPG) cotyledons and that the VTC2 and VTC5 promoters drove a day-to-night variation in expression. The present work provides clues to the precise roles of VTC2 and VTC5 in AsA biosynthesis in A. thaliana at the young seedling stage.

AB - Arabidopsis thaliana contains two GDP-L-galactose phosphorylase genes, VTC2 and VTC5, which are critical for ascorbate (AsA) biosynthesis. We investigated the expression levels of both VTC2 and VTC5 genes in wild-type A. thaliana and the AsA deficient mutants during early seedling growth. Ascorbate accumulated to an equal extent in all genotypes up to 5 d post-germination (DPG). The transcript level of VTC2 was dominant, and increased in parallel with AsA accumulation in the wild type. On the other hand, the expression of VTC5 compensated for the reduced VTC2 transcription levels in the AsA deficient mutant vtc2-1 in young seedlings. A luciferase activity assay indicated that the VTC5 promoter was more active in young (2 DPG) cotyledons and that the VTC2 and VTC5 promoters drove a day-to-night variation in expression. The present work provides clues to the precise roles of VTC2 and VTC5 in AsA biosynthesis in A. thaliana at the young seedling stage.

U2 - 10.1271/bbb.110320

DO - 10.1271/bbb.110320

M3 - Journal article

VL - 75

SP - 1783

EP - 1788

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 1347-6947

IS - 9

ER -