Expression and transport characterisation of the wheat low-affinity cation transporter (LCT1) in the methylotrophic yeast Pichia pastoris.

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Expression and transport characterisation of the wheat low-affinity cation transporter (LCT1) in the methylotrophic yeast Pichia pastoris. / Diatloff, Eugene; Forde, Brian G.; Roberts, Stephen K.
In: Biochemical and Biophysical Research Communications, Vol. 344, No. 3, 09.06.2006, p. 807-813.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Diatloff, E, Forde, BG & Roberts, SK 2006, 'Expression and transport characterisation of the wheat low-affinity cation transporter (LCT1) in the methylotrophic yeast Pichia pastoris.', Biochemical and Biophysical Research Communications, vol. 344, no. 3, pp. 807-813. https://doi.org/10.1016/j.bbrc.2006.03.212

APA

Diatloff, E., Forde, B. G., & Roberts, S. K. (2006). Expression and transport characterisation of the wheat low-affinity cation transporter (LCT1) in the methylotrophic yeast Pichia pastoris. Biochemical and Biophysical Research Communications, 344(3), 807-813. https://doi.org/10.1016/j.bbrc.2006.03.212

Vancouver

Diatloff E, Forde BG , Roberts SK. Expression and transport characterisation of the wheat low-affinity cation transporter (LCT1) in the methylotrophic yeast Pichia pastoris. Biochemical and Biophysical Research Communications. 2006 Jun 9;344(3):807-813. doi: 10.1016/j.bbrc.2006.03.212

Author

Diatloff, Eugene ; Forde, Brian G. ; Roberts, Stephen K. / Expression and transport characterisation of the wheat low-affinity cation transporter (LCT1) in the methylotrophic yeast Pichia pastoris. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 344, No. 3. pp. 807-813.

Bibtex

@article{08195cb01d564dfb9a0347c1388c5cdd,

title = "Expression and transport characterisation of the wheat low-affinity cation transporter (LCT1) in the methylotrophic yeast Pichia pastoris.",

abstract = "The low-affinity cation transporter (LCT1) from wheat (Triticum aestivum) was expressed in the methylotrophic yeast Pichia pastoris and its transport characteristics studied employing Ca45 and Cd109. A clone (LCT1#3) with the highest uptake of 14 pmol of Ca/106 cells/10 min when exposed to 100 μM Ca45 was chosen for further Ca45 and Cd109 transport characteristics. We report for the first time a Km for Ca by LCT1 of 0.43 ± 0.15 mM Ca activity which confirms LCT1 to be a low affinity transporter. Interestingly, the expression of LCT1 in Pichia resulted in reduced Cd109 uptake compared to wild type cells, when cells were exposed to 60 μM Cd. This is the first report of the ability of a heterologously expressed transporter to reduce the activity of endogenous transporter proteins to transport Cd. To our knowledge, this is the first demonstration of functional expression of a plant ion transporter using P. pastoris.",

keywords = "Cadmium, Calcium, Cation transporter, Heterologous expression, Low affinity, LCT1, Pichia pastoris, Triticum aestivum, Wheat",

author = "Eugene Diatloff and Forde, {Brian G.} and Roberts, {Stephen K.}",

year = "2006",

month = jun,

day = "9",

doi = "10.1016/j.bbrc.2006.03.212",

language = "English",

volume = "344",

pages = "807--813",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

RIS

TY - JOUR

T1 - Expression and transport characterisation of the wheat low-affinity cation transporter (LCT1) in the methylotrophic yeast Pichia pastoris.

AU - Diatloff, Eugene

AU - Forde, Brian G.

AU - Roberts, Stephen K.

PY - 2006/6/9

Y1 - 2006/6/9

N2 - The low-affinity cation transporter (LCT1) from wheat (Triticum aestivum) was expressed in the methylotrophic yeast Pichia pastoris and its transport characteristics studied employing Ca45 and Cd109. A clone (LCT1#3) with the highest uptake of 14 pmol of Ca/106 cells/10 min when exposed to 100 μM Ca45 was chosen for further Ca45 and Cd109 transport characteristics. We report for the first time a Km for Ca by LCT1 of 0.43 ± 0.15 mM Ca activity which confirms LCT1 to be a low affinity transporter. Interestingly, the expression of LCT1 in Pichia resulted in reduced Cd109 uptake compared to wild type cells, when cells were exposed to 60 μM Cd. This is the first report of the ability of a heterologously expressed transporter to reduce the activity of endogenous transporter proteins to transport Cd. To our knowledge, this is the first demonstration of functional expression of a plant ion transporter using P. pastoris.

AB - The low-affinity cation transporter (LCT1) from wheat (Triticum aestivum) was expressed in the methylotrophic yeast Pichia pastoris and its transport characteristics studied employing Ca45 and Cd109. A clone (LCT1#3) with the highest uptake of 14 pmol of Ca/106 cells/10 min when exposed to 100 μM Ca45 was chosen for further Ca45 and Cd109 transport characteristics. We report for the first time a Km for Ca by LCT1 of 0.43 ± 0.15 mM Ca activity which confirms LCT1 to be a low affinity transporter. Interestingly, the expression of LCT1 in Pichia resulted in reduced Cd109 uptake compared to wild type cells, when cells were exposed to 60 μM Cd. This is the first report of the ability of a heterologously expressed transporter to reduce the activity of endogenous transporter proteins to transport Cd. To our knowledge, this is the first demonstration of functional expression of a plant ion transporter using P. pastoris.

KW - Cadmium

KW - Calcium

KW - Cation transporter

KW - Heterologous expression

KW - Low affinity

KW - LCT1

KW - Pichia pastoris

KW - Triticum aestivum

KW - Wheat

U2 - 10.1016/j.bbrc.2006.03.212

DO - 10.1016/j.bbrc.2006.03.212

M3 - Journal article

VL - 344

SP - 807

EP - 813

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -

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