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Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi

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Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi. / Suwan, N; Wilkinson, M C; Crampton, J M et al.
In: Insect Molecular Biology, Vol. 11, No. 3, 06.2002, p. 223-232.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Suwan, N, Wilkinson, MC, Crampton, JM & Bates, PA 2002, 'Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi', Insect Molecular Biology, vol. 11, no. 3, pp. 223-232. https://doi.org/10.1046/j.1365-2583.2002.00329.x

APA

Suwan, N., Wilkinson, M. C., Crampton, J. M., & Bates, P. A. (2002). Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi. Insect Molecular Biology, 11(3), 223-232. https://doi.org/10.1046/j.1365-2583.2002.00329.x

Vancouver

Suwan N, Wilkinson MC, Crampton JM, Bates PA. Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi. Insect Molecular Biology. 2002 Jun;11(3):223-232. doi: 10.1046/j.1365-2583.2002.00329.x

Author

Suwan, N ; Wilkinson, M C ; Crampton, J M et al. / Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi. In: Insect Molecular Biology. 2002 ; Vol. 11, No. 3. pp. 223-232.

Bibtex

@article{70ec3d49ac314d22967f91e31324d443,
title = "Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi",
abstract = "Full-length cDNA clones encoding D7 (AnsD7) and D7-related (AnsD7r1) secreted salivary gland proteins were isolated from Anopheles stephensi. Corresponding proteins were separated by SDS-PAGE and analysed by N-terminal sequencing, which also identified a second D7-related protein (AnsD7r2). AnsD7 encodes a protein of 37 kDa, AnsD7r1 of 18 kDa, and AnsD7r2 of 16 kDa. Polyclonal antibodies against recombinant AnsD7 showed immunological cross-reactivity with the D7-related proteins, and alignment demonstrated sequence similarity between the C-terminal region of AnsD7 and the D7-related proteins. AnsD7, AnsD7r1 and AnsD7r2 were major female-specific salivary gland proteins, and Western blotting, immunohistochemistry and immunogold labelling demonstrated expression was predominantly in the secretory cavities of the distal-lateral and median lobes. Expression and localization of D7 and D7-related proteins was similar in Plasmodium berghei-infected and uninfected mosquitoes.",
keywords = "Anopheles stephensi, salivary glands , mosquito , Malaria",
author = "N Suwan and Wilkinson, {M C} and Crampton, {J M} and Bates, {P A}",
year = "2002",
month = jun,
doi = "10.1046/j.1365-2583.2002.00329.x",
language = "English",
volume = "11",
pages = "223--232",
journal = "Insect Molecular Biology",
issn = "1365-2583",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi

AU - Suwan, N

AU - Wilkinson, M C

AU - Crampton, J M

AU - Bates, P A

PY - 2002/6

Y1 - 2002/6

N2 - Full-length cDNA clones encoding D7 (AnsD7) and D7-related (AnsD7r1) secreted salivary gland proteins were isolated from Anopheles stephensi. Corresponding proteins were separated by SDS-PAGE and analysed by N-terminal sequencing, which also identified a second D7-related protein (AnsD7r2). AnsD7 encodes a protein of 37 kDa, AnsD7r1 of 18 kDa, and AnsD7r2 of 16 kDa. Polyclonal antibodies against recombinant AnsD7 showed immunological cross-reactivity with the D7-related proteins, and alignment demonstrated sequence similarity between the C-terminal region of AnsD7 and the D7-related proteins. AnsD7, AnsD7r1 and AnsD7r2 were major female-specific salivary gland proteins, and Western blotting, immunohistochemistry and immunogold labelling demonstrated expression was predominantly in the secretory cavities of the distal-lateral and median lobes. Expression and localization of D7 and D7-related proteins was similar in Plasmodium berghei-infected and uninfected mosquitoes.

AB - Full-length cDNA clones encoding D7 (AnsD7) and D7-related (AnsD7r1) secreted salivary gland proteins were isolated from Anopheles stephensi. Corresponding proteins were separated by SDS-PAGE and analysed by N-terminal sequencing, which also identified a second D7-related protein (AnsD7r2). AnsD7 encodes a protein of 37 kDa, AnsD7r1 of 18 kDa, and AnsD7r2 of 16 kDa. Polyclonal antibodies against recombinant AnsD7 showed immunological cross-reactivity with the D7-related proteins, and alignment demonstrated sequence similarity between the C-terminal region of AnsD7 and the D7-related proteins. AnsD7, AnsD7r1 and AnsD7r2 were major female-specific salivary gland proteins, and Western blotting, immunohistochemistry and immunogold labelling demonstrated expression was predominantly in the secretory cavities of the distal-lateral and median lobes. Expression and localization of D7 and D7-related proteins was similar in Plasmodium berghei-infected and uninfected mosquitoes.

KW - Anopheles stephensi

KW - salivary glands

KW - mosquito

KW - Malaria

U2 - 10.1046/j.1365-2583.2002.00329.x

DO - 10.1046/j.1365-2583.2002.00329.x

M3 - Journal article

C2 - 12000641

VL - 11

SP - 223

EP - 232

JO - Insect Molecular Biology

JF - Insect Molecular Biology

SN - 1365-2583

IS - 3

ER -