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Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides

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Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides. / Popplewell, Jonathan; Swann, Marcus; Brown, Gavin; Lauder, Bob.

In: Methods in Molecular Biology, Vol. 808, 01.01.2012, p. 221-229.

Research output: Contribution to journalJournal article

Harvard

Popplewell, J, Swann, M, Brown, G & Lauder, B 2012, 'Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides', Methods in Molecular Biology, vol. 808, pp. 221-229. https://doi.org/10.1007/978-1-61779-373-8_15

APA

Popplewell, J., Swann, M., Brown, G., & Lauder, B. (2012). Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides. Methods in Molecular Biology, 808, 221-229. https://doi.org/10.1007/978-1-61779-373-8_15

Vancouver

Popplewell J, Swann M, Brown G, Lauder B. Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides. Methods in Molecular Biology. 2012 Jan 1;808:221-229. https://doi.org/10.1007/978-1-61779-373-8_15

Author

Popplewell, Jonathan ; Swann, Marcus ; Brown, Gavin ; Lauder, Bob. / Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides. In: Methods in Molecular Biology. 2012 ; Vol. 808. pp. 221-229.

Bibtex

@article{46aaa26b09e643be961a9e46f5a143ed,
title = "Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides",
abstract = "Surface-based tools, such as microarrays and optical biosensors, are being increasingly applied to the analysis of carbohydrate-protein interactions. A key to these developments is the presentation of the carbohydrate to the protein target. Dual polarisation interferometry (DPI) is a surface-based technique that permits the real-time measurement of the changes in thickness, refractive index, and mass of adsorbates 100-nm thick or less on the surface of a functionalised waveguide. DPI has been used to design and characterise a surface on which the orientation and density of the immobilised carbohydrates are suitable for studying their interactions with proteins and where non-specific binding is reduced to less than 5% of total binding. A thiol-functionalised surface was derivatised with a heterobifunctional cross-linker to yield a hydrazide surface. This was treated with oligosaccharides, derived from keratan sulphate, chondroitin sulphate, and heparin that possess a reducing end. To block the unreacted hydrazide groups, the surface was treated with an aldehyde-functionalised PEG, and the surfaces were then challenged with a variety of proteins.",
keywords = "Chondroitin, Keratan",
author = "Jonathan Popplewell and Marcus Swann and Gavin Brown and Bob Lauder",
year = "2012",
month = jan,
day = "1",
doi = "10.1007/978-1-61779-373-8_15",
language = "English",
volume = "808",
pages = "221--229",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides

AU - Popplewell, Jonathan

AU - Swann, Marcus

AU - Brown, Gavin

AU - Lauder, Bob

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Surface-based tools, such as microarrays and optical biosensors, are being increasingly applied to the analysis of carbohydrate-protein interactions. A key to these developments is the presentation of the carbohydrate to the protein target. Dual polarisation interferometry (DPI) is a surface-based technique that permits the real-time measurement of the changes in thickness, refractive index, and mass of adsorbates 100-nm thick or less on the surface of a functionalised waveguide. DPI has been used to design and characterise a surface on which the orientation and density of the immobilised carbohydrates are suitable for studying their interactions with proteins and where non-specific binding is reduced to less than 5% of total binding. A thiol-functionalised surface was derivatised with a heterobifunctional cross-linker to yield a hydrazide surface. This was treated with oligosaccharides, derived from keratan sulphate, chondroitin sulphate, and heparin that possess a reducing end. To block the unreacted hydrazide groups, the surface was treated with an aldehyde-functionalised PEG, and the surfaces were then challenged with a variety of proteins.

AB - Surface-based tools, such as microarrays and optical biosensors, are being increasingly applied to the analysis of carbohydrate-protein interactions. A key to these developments is the presentation of the carbohydrate to the protein target. Dual polarisation interferometry (DPI) is a surface-based technique that permits the real-time measurement of the changes in thickness, refractive index, and mass of adsorbates 100-nm thick or less on the surface of a functionalised waveguide. DPI has been used to design and characterise a surface on which the orientation and density of the immobilised carbohydrates are suitable for studying their interactions with proteins and where non-specific binding is reduced to less than 5% of total binding. A thiol-functionalised surface was derivatised with a heterobifunctional cross-linker to yield a hydrazide surface. This was treated with oligosaccharides, derived from keratan sulphate, chondroitin sulphate, and heparin that possess a reducing end. To block the unreacted hydrazide groups, the surface was treated with an aldehyde-functionalised PEG, and the surfaces were then challenged with a variety of proteins.

KW - Chondroitin

KW - Keratan

U2 - 10.1007/978-1-61779-373-8_15

DO - 10.1007/978-1-61779-373-8_15

M3 - Journal article

C2 - 22057528

VL - 808

SP - 221

EP - 229

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -