Rights statement: This is the author’s version of a work that was accepted for publication in International Journal for Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Joural for Parasitology, 45, 11, 2015 DOI: 10.1016/j.ijpara.2015.05.001
Accepted author manuscript, 1.54 MB, PDF document
Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
13.9 KB, PDF document
Final published version
Licence: CC BY: Creative Commons Attribution 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex
AU - Kwakye-Nuako, Godwin
AU - Mosore, Mba-Tihssommah
AU - Duplessis, Christopher
AU - Bates, Michelle
AU - Puplampu, Naiki
AU - Mensah-Attipoe, Israel
AU - Desewu, Kwame
AU - Afegbe, Godwin
AU - Asmah, Richard
AU - Jamjoom, Manal
AU - Ayeh-Kumi, Patrick
AU - Boakye, Daniel
AU - Bates, Paul
N1 - This is the author’s version of a work that was accepted for publication in International Journal for Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Joural for Parasitology, 45, 11, 2015 DOI: 10.1016/j.ijpara.2015.05.001
PY - 2015/9
Y1 - 2015/9
N2 - An active case detection approach with PCR diagnosis was used in the Ho District of the Volta Region, Ghana that identified individuals with active cutaneous leishmaniasis. Three isolates were successfully cultured and DNA sequences from these were analysed (ribosomal RNA internal transcribed spacer 1; ribosomal protein L23a intergenic spacer; RNA polymerase II large subunit), showing them to be Leishmania, identical to each other but different from all other known Leishmania spp. Phylogenetic analysis showed the parasites to be new members of the Leishmania enriettii complex, which is emerging as a possible new subgenus of Leishmania parasites containing human pathogens.
AB - An active case detection approach with PCR diagnosis was used in the Ho District of the Volta Region, Ghana that identified individuals with active cutaneous leishmaniasis. Three isolates were successfully cultured and DNA sequences from these were analysed (ribosomal RNA internal transcribed spacer 1; ribosomal protein L23a intergenic spacer; RNA polymerase II large subunit), showing them to be Leishmania, identical to each other but different from all other known Leishmania spp. Phylogenetic analysis showed the parasites to be new members of the Leishmania enriettii complex, which is emerging as a possible new subgenus of Leishmania parasites containing human pathogens.
KW - Ghana
KW - Leishmania
KW - Cutaneous leishmaniasis
KW - Phylogeny
U2 - 10.1016/j.ijpara.2015.05.001
DO - 10.1016/j.ijpara.2015.05.001
M3 - Journal article
VL - 45
SP - 679
EP - 684
JO - International Journal for Parasitology
JF - International Journal for Parasitology
SN - 0020-7519
IS - 11
ER -