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Flagellar attachment of Leishmania promastigotes to plastic film in vitro

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Flagellar attachment of Leishmania promastigotes to plastic film in vitro. / Wakid, Majed H; Bates, Paul A.
In: Experimental Parasitology, Vol. 106, No. 3-4, 03.2004, p. 173-178.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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Wakid MH, Bates PA. Flagellar attachment of Leishmania promastigotes to plastic film in vitro. Experimental Parasitology. 2004 Mar;106(3-4):173-178. doi: 10.1016/j.exppara.2004.03.001

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Wakid, Majed H ; Bates, Paul A. / Flagellar attachment of Leishmania promastigotes to plastic film in vitro. In: Experimental Parasitology. 2004 ; Vol. 106, No. 3-4. pp. 173-178.

Bibtex

@article{b1d596cd979d4b459f6ada978b71a9f8,
title = "Flagellar attachment of Leishmania promastigotes to plastic film in vitro",
abstract = "Trypanosomatid parasites are able to use their flagella for attachment to cuticular surfaces within their arthropod hosts. In this study the attachment mechanism of Leishmania promastigotes was investigated using a new and quantifiable in vitro assay system. The results showed that hemidesmosomal flagellar attachment to three different plastic substrates occurred (Melinex, Polyvinyl, Thermanox). Attachment density was increased by scratching the surface of the substrate or by coating with the hydrocarbons n-octacosane and paraffin. Variation in attachment density was observed, depending on the culture medium and the parasite isolate used. All four species examined, L. braziliensis, L. donovani, L. major and L. mexicana, were capable of flagellar attachment in vitro. Collectively, these data indicate that flagellar attachment is mediated by a non-specific hydrophobic interaction in Leishmania species.",
author = "Wakid, {Majed H} and Bates, {Paul A}",
year = "2004",
month = mar,
doi = "10.1016/j.exppara.2004.03.001",
language = "English",
volume = "106",
pages = "173--178",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",
number = "3-4",

}

RIS

TY - JOUR

T1 - Flagellar attachment of Leishmania promastigotes to plastic film in vitro

AU - Wakid, Majed H

AU - Bates, Paul A

PY - 2004/3

Y1 - 2004/3

N2 - Trypanosomatid parasites are able to use their flagella for attachment to cuticular surfaces within their arthropod hosts. In this study the attachment mechanism of Leishmania promastigotes was investigated using a new and quantifiable in vitro assay system. The results showed that hemidesmosomal flagellar attachment to three different plastic substrates occurred (Melinex, Polyvinyl, Thermanox). Attachment density was increased by scratching the surface of the substrate or by coating with the hydrocarbons n-octacosane and paraffin. Variation in attachment density was observed, depending on the culture medium and the parasite isolate used. All four species examined, L. braziliensis, L. donovani, L. major and L. mexicana, were capable of flagellar attachment in vitro. Collectively, these data indicate that flagellar attachment is mediated by a non-specific hydrophobic interaction in Leishmania species.

AB - Trypanosomatid parasites are able to use their flagella for attachment to cuticular surfaces within their arthropod hosts. In this study the attachment mechanism of Leishmania promastigotes was investigated using a new and quantifiable in vitro assay system. The results showed that hemidesmosomal flagellar attachment to three different plastic substrates occurred (Melinex, Polyvinyl, Thermanox). Attachment density was increased by scratching the surface of the substrate or by coating with the hydrocarbons n-octacosane and paraffin. Variation in attachment density was observed, depending on the culture medium and the parasite isolate used. All four species examined, L. braziliensis, L. donovani, L. major and L. mexicana, were capable of flagellar attachment in vitro. Collectively, these data indicate that flagellar attachment is mediated by a non-specific hydrophobic interaction in Leishmania species.

U2 - 10.1016/j.exppara.2004.03.001

DO - 10.1016/j.exppara.2004.03.001

M3 - Journal article

C2 - 15172225

VL - 106

SP - 173

EP - 178

JO - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 3-4

ER -