Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Fourier transform infrared spectroscopic analysis of breast cancer tissues; Identifying differences between normal breast, invasive ductal carcinoma, and ductal carcinoma in situ of the breast
AU - Rehman, S.
AU - Movasaghi, Z.
AU - Darr, J.A.
AU - Rehman, I.U.
PY - 2010
Y1 - 2010
N2 - A nondestructive method employing Fourier transform infrared (FTIR) microspectroscopy coupled with attenuated total reflectance (ATR) objective for the analysis of histopathological specimens is described. Malignant breast tissue specimens have been analyzed to demonstrate the hypothesis that chemical changes taking place in biological tissue can be reliably and reproducibly identified. This study is the first report to elucidate clear spectral differences between different ductal carcinoma in situ (DCIS) grades. Sixty individual cases of breast carcinoma including DCIS and invasive ductal carcinoma (IDC) and seven cases of normal breast tissues were studied using the FTIR-ATR spectroscopic technique. FTIR analysis of tissue sections has provided distinct spectra that can be used to distinguish between the nuclear grades of DCIS and IDC of the breast. It was concluded that FTIR could objectively and reproducibly discriminate between DCIS and IDC grades without sample destruction. In the future, applications of FTIR approaches should become feasible in the nondestructive express classification of grades and diagnosis of breast carcinoma. Copyright © Taylor & Francis Group, LLC.
AB - A nondestructive method employing Fourier transform infrared (FTIR) microspectroscopy coupled with attenuated total reflectance (ATR) objective for the analysis of histopathological specimens is described. Malignant breast tissue specimens have been analyzed to demonstrate the hypothesis that chemical changes taking place in biological tissue can be reliably and reproducibly identified. This study is the first report to elucidate clear spectral differences between different ductal carcinoma in situ (DCIS) grades. Sixty individual cases of breast carcinoma including DCIS and invasive ductal carcinoma (IDC) and seven cases of normal breast tissues were studied using the FTIR-ATR spectroscopic technique. FTIR analysis of tissue sections has provided distinct spectra that can be used to distinguish between the nuclear grades of DCIS and IDC of the breast. It was concluded that FTIR could objectively and reproducibly discriminate between DCIS and IDC grades without sample destruction. In the future, applications of FTIR approaches should become feasible in the nondestructive express classification of grades and diagnosis of breast carcinoma. Copyright © Taylor & Francis Group, LLC.
KW - breast cancer
KW - breast carcinoma
KW - ductal carcinoma in situ (DCIS)
KW - FTIR-ATR spectroscopy
KW - invasive ductal carcinoma (IDC)
KW - Breast Cancer
KW - Ductal carcinoma
KW - Ductal carcinoma in situ
KW - Histology
KW - Spectroscopic analysis
KW - Tissue
KW - Fourier transforms
U2 - 10.1080/05704928.2010.483674
DO - 10.1080/05704928.2010.483674
M3 - Journal article
VL - 45
SP - 355
EP - 368
JO - APPLIED SPECTROSCOPY REVIEWS
JF - APPLIED SPECTROSCOPY REVIEWS
SN - 0570-4928
IS - 5
ER -