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Freeze gelated porous membranes for periodontal tissue regeneration

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Freeze gelated porous membranes for periodontal tissue regeneration. / Qasim, S.B.; Delaine-Smith, R.M.; Fey, T.; Rawlinson, A.; Rehman, I.U.

In: Acta Biomaterialia, Vol. 23, 01.09.2015, p. 317-328.

Research output: Contribution to journalJournal article

Harvard

Qasim, SB, Delaine-Smith, RM, Fey, T, Rawlinson, A & Rehman, IU 2015, 'Freeze gelated porous membranes for periodontal tissue regeneration', Acta Biomaterialia, vol. 23, pp. 317-328. https://doi.org/10.1016/j.actbio.2015.05.001

APA

Qasim, S. B., Delaine-Smith, R. M., Fey, T., Rawlinson, A., & Rehman, I. U. (2015). Freeze gelated porous membranes for periodontal tissue regeneration. Acta Biomaterialia, 23, 317-328. https://doi.org/10.1016/j.actbio.2015.05.001

Vancouver

Qasim SB, Delaine-Smith RM, Fey T, Rawlinson A, Rehman IU. Freeze gelated porous membranes for periodontal tissue regeneration. Acta Biomaterialia. 2015 Sep 1;23:317-328. https://doi.org/10.1016/j.actbio.2015.05.001

Author

Qasim, S.B. ; Delaine-Smith, R.M. ; Fey, T. ; Rawlinson, A. ; Rehman, I.U. / Freeze gelated porous membranes for periodontal tissue regeneration. In: Acta Biomaterialia. 2015 ; Vol. 23. pp. 317-328.

Bibtex

@article{6170afdaf7714ca89e917ee3ecd21434,
title = "Freeze gelated porous membranes for periodontal tissue regeneration",
abstract = "Guided tissue regeneration (GTR) membranes have been used for the management of destructive forms of periodontal disease as a means of aiding regeneration of lost supporting tissues, including the alveolar bone, cementum, gingiva and periodontal ligaments (PDL). Currently available GTR membranes are either non-biodegradable, requiring a second surgery for removal, or biodegradable. The mechanical and biofunctional limitations of currently available membranes result in a limited and unpredictable treatment outcome in terms of periodontal tissue regeneration. In this study, porous membranes of chitosan (CH) were fabricated with or without hydroxyapatite (HA) using the simple technique of freeze gelation (FG) via two different solvents systems, acetic acid (ACa) or ascorbic acid (ASa). The aim was to prepare porous membranes to be used for GTR to improve periodontal regeneration. FG membranes were characterized for ultra-structural morphology, physiochemical properties, water uptake, degradation, mechanical properties, and biocompatibility with mature and progenitor osteogenic cells. Fourier transform infrared (FTIR) spectroscopy confirmed the presence of hydroxyapatite and its interaction with chitosan. μCT analysis showed membranes had 85-77% porosity. Mechanical properties and degradation rate were affected by solvent type and the presence of hydroxyapatite. Culture of human osteosarcoma cells (MG63) and human embryonic stem cell-derived mesenchymal progenitors (hES-MPs) showed that all membranes supported cell proliferation and long term matrix deposition was supported by HA incorporated membranes. These CH and HA composite membranes show their potential use for GTR applications in periodontal lesions and in addition FG membranes could be further tuned to achieve characteristics desirable of a GTR membrane for periodontal regeneration. {\textcopyright} 2015 Acta Materialia Inc.",
keywords = "Ascorbic acid, Bioactivity, Guided tissue regeneration, Osteoblasts, Resorbable, acetic acid, ascorbic acid, calcium, chitosan, hydroxyapatite, artificial membrane, gel, Article, biocompatibility, cell proliferation, controlled study, degradation, embryo, embryonic stem cell, Fourier transform infrared photoacoustic spectroscopy, freeze gelation porous membrane, gelation, human, human cell, mechanics, mesenchymal stem cell, molecular weight, osteoblast, osteosarcoma cell, periodontal disease, periodontal ligament, physical chemistry, porosity, priority journal, stem cell, tensile strength, tissue regeneration, tissue scaffold, water transport, bone development, cell culture, chemistry, cytology, device failure analysis, devices, equipment design, freezing, materials testing, mechanical stress, periodontics, physiology, procedures, Young modulus, Cell Proliferation, Cells, Cultured, Chitosan, Durapatite, Elastic Modulus, Equipment Design, Equipment Failure Analysis, Freezing, Gels, Guided Tissue Regeneration, Periodontal, Humans, Materials Testing, Membranes, Artificial, Osteogenesis, Porosity, Stem Cells, Stress, Mechanical, Tensile Strength, Tissue Scaffolds",
author = "S.B. Qasim and R.M. Delaine-Smith and T. Fey and A. Rawlinson and I.U. Rehman",
year = "2015",
month = sep,
day = "1",
doi = "10.1016/j.actbio.2015.05.001",
language = "English",
volume = "23",
pages = "317--328",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Freeze gelated porous membranes for periodontal tissue regeneration

AU - Qasim, S.B.

AU - Delaine-Smith, R.M.

AU - Fey, T.

AU - Rawlinson, A.

AU - Rehman, I.U.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Guided tissue regeneration (GTR) membranes have been used for the management of destructive forms of periodontal disease as a means of aiding regeneration of lost supporting tissues, including the alveolar bone, cementum, gingiva and periodontal ligaments (PDL). Currently available GTR membranes are either non-biodegradable, requiring a second surgery for removal, or biodegradable. The mechanical and biofunctional limitations of currently available membranes result in a limited and unpredictable treatment outcome in terms of periodontal tissue regeneration. In this study, porous membranes of chitosan (CH) were fabricated with or without hydroxyapatite (HA) using the simple technique of freeze gelation (FG) via two different solvents systems, acetic acid (ACa) or ascorbic acid (ASa). The aim was to prepare porous membranes to be used for GTR to improve periodontal regeneration. FG membranes were characterized for ultra-structural morphology, physiochemical properties, water uptake, degradation, mechanical properties, and biocompatibility with mature and progenitor osteogenic cells. Fourier transform infrared (FTIR) spectroscopy confirmed the presence of hydroxyapatite and its interaction with chitosan. μCT analysis showed membranes had 85-77% porosity. Mechanical properties and degradation rate were affected by solvent type and the presence of hydroxyapatite. Culture of human osteosarcoma cells (MG63) and human embryonic stem cell-derived mesenchymal progenitors (hES-MPs) showed that all membranes supported cell proliferation and long term matrix deposition was supported by HA incorporated membranes. These CH and HA composite membranes show their potential use for GTR applications in periodontal lesions and in addition FG membranes could be further tuned to achieve characteristics desirable of a GTR membrane for periodontal regeneration. © 2015 Acta Materialia Inc.

AB - Guided tissue regeneration (GTR) membranes have been used for the management of destructive forms of periodontal disease as a means of aiding regeneration of lost supporting tissues, including the alveolar bone, cementum, gingiva and periodontal ligaments (PDL). Currently available GTR membranes are either non-biodegradable, requiring a second surgery for removal, or biodegradable. The mechanical and biofunctional limitations of currently available membranes result in a limited and unpredictable treatment outcome in terms of periodontal tissue regeneration. In this study, porous membranes of chitosan (CH) were fabricated with or without hydroxyapatite (HA) using the simple technique of freeze gelation (FG) via two different solvents systems, acetic acid (ACa) or ascorbic acid (ASa). The aim was to prepare porous membranes to be used for GTR to improve periodontal regeneration. FG membranes were characterized for ultra-structural morphology, physiochemical properties, water uptake, degradation, mechanical properties, and biocompatibility with mature and progenitor osteogenic cells. Fourier transform infrared (FTIR) spectroscopy confirmed the presence of hydroxyapatite and its interaction with chitosan. μCT analysis showed membranes had 85-77% porosity. Mechanical properties and degradation rate were affected by solvent type and the presence of hydroxyapatite. Culture of human osteosarcoma cells (MG63) and human embryonic stem cell-derived mesenchymal progenitors (hES-MPs) showed that all membranes supported cell proliferation and long term matrix deposition was supported by HA incorporated membranes. These CH and HA composite membranes show their potential use for GTR applications in periodontal lesions and in addition FG membranes could be further tuned to achieve characteristics desirable of a GTR membrane for periodontal regeneration. © 2015 Acta Materialia Inc.

KW - Ascorbic acid

KW - Bioactivity

KW - Guided tissue regeneration

KW - Osteoblasts

KW - Resorbable

KW - acetic acid

KW - ascorbic acid

KW - calcium

KW - chitosan

KW - hydroxyapatite

KW - artificial membrane

KW - gel

KW - Article

KW - biocompatibility

KW - cell proliferation

KW - controlled study

KW - degradation

KW - embryo

KW - embryonic stem cell

KW - Fourier transform infrared photoacoustic spectroscopy

KW - freeze gelation porous membrane

KW - gelation

KW - human

KW - human cell

KW - mechanics

KW - mesenchymal stem cell

KW - molecular weight

KW - osteoblast

KW - osteosarcoma cell

KW - periodontal disease

KW - periodontal ligament

KW - physical chemistry

KW - porosity

KW - priority journal

KW - stem cell

KW - tensile strength

KW - tissue regeneration

KW - tissue scaffold

KW - water transport

KW - bone development

KW - cell culture

KW - chemistry

KW - cytology

KW - device failure analysis

KW - devices

KW - equipment design

KW - freezing

KW - materials testing

KW - mechanical stress

KW - periodontics

KW - physiology

KW - procedures

KW - Young modulus

KW - Cell Proliferation

KW - Cells, Cultured

KW - Chitosan

KW - Durapatite

KW - Elastic Modulus

KW - Equipment Design

KW - Equipment Failure Analysis

KW - Freezing

KW - Gels

KW - Guided Tissue Regeneration, Periodontal

KW - Humans

KW - Materials Testing

KW - Membranes, Artificial

KW - Osteogenesis

KW - Porosity

KW - Stem Cells

KW - Stress, Mechanical

KW - Tensile Strength

KW - Tissue Scaffolds

U2 - 10.1016/j.actbio.2015.05.001

DO - 10.1016/j.actbio.2015.05.001

M3 - Journal article

VL - 23

SP - 317

EP - 328

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

ER -