Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Freeze gelated porous membranes for periodontal tissue regeneration
AU - Qasim, S.B.
AU - Delaine-Smith, R.M.
AU - Fey, T.
AU - Rawlinson, A.
AU - Rehman, I.U.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Guided tissue regeneration (GTR) membranes have been used for the management of destructive forms of periodontal disease as a means of aiding regeneration of lost supporting tissues, including the alveolar bone, cementum, gingiva and periodontal ligaments (PDL). Currently available GTR membranes are either non-biodegradable, requiring a second surgery for removal, or biodegradable. The mechanical and biofunctional limitations of currently available membranes result in a limited and unpredictable treatment outcome in terms of periodontal tissue regeneration. In this study, porous membranes of chitosan (CH) were fabricated with or without hydroxyapatite (HA) using the simple technique of freeze gelation (FG) via two different solvents systems, acetic acid (ACa) or ascorbic acid (ASa). The aim was to prepare porous membranes to be used for GTR to improve periodontal regeneration. FG membranes were characterized for ultra-structural morphology, physiochemical properties, water uptake, degradation, mechanical properties, and biocompatibility with mature and progenitor osteogenic cells. Fourier transform infrared (FTIR) spectroscopy confirmed the presence of hydroxyapatite and its interaction with chitosan. μCT analysis showed membranes had 85-77% porosity. Mechanical properties and degradation rate were affected by solvent type and the presence of hydroxyapatite. Culture of human osteosarcoma cells (MG63) and human embryonic stem cell-derived mesenchymal progenitors (hES-MPs) showed that all membranes supported cell proliferation and long term matrix deposition was supported by HA incorporated membranes. These CH and HA composite membranes show their potential use for GTR applications in periodontal lesions and in addition FG membranes could be further tuned to achieve characteristics desirable of a GTR membrane for periodontal regeneration. © 2015 Acta Materialia Inc.
AB - Guided tissue regeneration (GTR) membranes have been used for the management of destructive forms of periodontal disease as a means of aiding regeneration of lost supporting tissues, including the alveolar bone, cementum, gingiva and periodontal ligaments (PDL). Currently available GTR membranes are either non-biodegradable, requiring a second surgery for removal, or biodegradable. The mechanical and biofunctional limitations of currently available membranes result in a limited and unpredictable treatment outcome in terms of periodontal tissue regeneration. In this study, porous membranes of chitosan (CH) were fabricated with or without hydroxyapatite (HA) using the simple technique of freeze gelation (FG) via two different solvents systems, acetic acid (ACa) or ascorbic acid (ASa). The aim was to prepare porous membranes to be used for GTR to improve periodontal regeneration. FG membranes were characterized for ultra-structural morphology, physiochemical properties, water uptake, degradation, mechanical properties, and biocompatibility with mature and progenitor osteogenic cells. Fourier transform infrared (FTIR) spectroscopy confirmed the presence of hydroxyapatite and its interaction with chitosan. μCT analysis showed membranes had 85-77% porosity. Mechanical properties and degradation rate were affected by solvent type and the presence of hydroxyapatite. Culture of human osteosarcoma cells (MG63) and human embryonic stem cell-derived mesenchymal progenitors (hES-MPs) showed that all membranes supported cell proliferation and long term matrix deposition was supported by HA incorporated membranes. These CH and HA composite membranes show their potential use for GTR applications in periodontal lesions and in addition FG membranes could be further tuned to achieve characteristics desirable of a GTR membrane for periodontal regeneration. © 2015 Acta Materialia Inc.
KW - Ascorbic acid
KW - Bioactivity
KW - Guided tissue regeneration
KW - Osteoblasts
KW - Resorbable
KW - acetic acid
KW - ascorbic acid
KW - calcium
KW - chitosan
KW - hydroxyapatite
KW - artificial membrane
KW - gel
KW - Article
KW - biocompatibility
KW - cell proliferation
KW - controlled study
KW - degradation
KW - embryo
KW - embryonic stem cell
KW - Fourier transform infrared photoacoustic spectroscopy
KW - freeze gelation porous membrane
KW - gelation
KW - human
KW - human cell
KW - mechanics
KW - mesenchymal stem cell
KW - molecular weight
KW - osteoblast
KW - osteosarcoma cell
KW - periodontal disease
KW - periodontal ligament
KW - physical chemistry
KW - porosity
KW - priority journal
KW - stem cell
KW - tensile strength
KW - tissue regeneration
KW - tissue scaffold
KW - water transport
KW - bone development
KW - cell culture
KW - chemistry
KW - cytology
KW - device failure analysis
KW - devices
KW - equipment design
KW - freezing
KW - materials testing
KW - mechanical stress
KW - periodontics
KW - physiology
KW - procedures
KW - Young modulus
KW - Cell Proliferation
KW - Cells, Cultured
KW - Chitosan
KW - Durapatite
KW - Elastic Modulus
KW - Equipment Design
KW - Equipment Failure Analysis
KW - Freezing
KW - Gels
KW - Guided Tissue Regeneration, Periodontal
KW - Humans
KW - Materials Testing
KW - Membranes, Artificial
KW - Osteogenesis
KW - Porosity
KW - Stem Cells
KW - Stress, Mechanical
KW - Tensile Strength
KW - Tissue Scaffolds
U2 - 10.1016/j.actbio.2015.05.001
DO - 10.1016/j.actbio.2015.05.001
M3 - Journal article
VL - 23
SP - 317
EP - 328
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
ER -