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Functional genomics of ozone stress in Arabidopsis.

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Functional genomics of ozone stress in Arabidopsis. / Short, E.; Shirras, A. D.; McAinsh, Martin.
In: Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Vol. 141, No. 3 Supp, 07.2005, p. S273-S274.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Short, E, Shirras, AD & McAinsh, M 2005, 'Functional genomics of ozone stress in Arabidopsis.', Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, vol. 141, no. 3 Supp, pp. S273-S274. <http://www.sciencedirect.com/science/journal/10956433>

APA

Short, E., Shirras, A. D., & McAinsh, M. (2005). Functional genomics of ozone stress in Arabidopsis. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 141(3 Supp), S273-S274. http://www.sciencedirect.com/science/journal/10956433

Vancouver

Short E, Shirras AD, McAinsh M. Functional genomics of ozone stress in Arabidopsis. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology. 2005 Jul;141(3 Supp):S273-S274.

Author

Short, E. ; Shirras, A. D. ; McAinsh, Martin. / Functional genomics of ozone stress in Arabidopsis. In: Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology. 2005 ; Vol. 141, No. 3 Supp. pp. S273-S274.

Bibtex

@article{41cb42015fbe48b5aae9d4461d23d8ec,
title = "Functional genomics of ozone stress in Arabidopsis.",
abstract = "Ozone is an important pollutant that has significant and frequently deleterious effects on plant health resulting in a reduction in cropy ields. This is due largely to oxidative damage caused either directly by ozone or by the reactive oxygen species generated by ozone. Plants possess a range of antioxidant defences to protect them against oxidative stress such as that caused by ozone and the expression of a wide-range of genes, including genes encoding important antioxidants, is altered by exposure to ozone. However, little is known about the signal transduction pathways linking the perception of ozone stress to gene expression. We have used a genomics approach to identify novel genes that respond to acute ozone stress in Arabidopsis thaliana. Initial transcript profiling studies using microarray analysis identified twenty genes which were highly up-regulated and one gene that was down-regulated by ozone treatment. Real Time RT-PCR and additional microarray analysis have subsequently confirmed this result. We have analyzed the expression of five of these genes in detail. Our studies show that the induction of all of these genes in response to oxidative stress was dependent on an increase in cytosolic free calcium and was specific to ozone. Importantly, they were not induced by other calcium-mobilizing oxidative stresses including hydrogen peroxide and cold. These data raise important questions about the mechanism by which these genes are induced by ozone and how stimulus specificity is encoded in the ozone calcium signature.",
keywords = "Arabidopsis, Ozone, Microarray, Calcium, Signalling specificity",
author = "E. Short and Shirras, {A. D.} and Martin McAinsh",
year = "2005",
month = jul,
language = "English",
volume = "141",
pages = "S273--S274",
journal = "Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology",
issn = "1095-6433",
publisher = "Elsevier Inc.",
number = "3 Supp",

}

RIS

TY - JOUR

T1 - Functional genomics of ozone stress in Arabidopsis.

AU - Short, E.

AU - Shirras, A. D.

AU - McAinsh, Martin

PY - 2005/7

Y1 - 2005/7

N2 - Ozone is an important pollutant that has significant and frequently deleterious effects on plant health resulting in a reduction in cropy ields. This is due largely to oxidative damage caused either directly by ozone or by the reactive oxygen species generated by ozone. Plants possess a range of antioxidant defences to protect them against oxidative stress such as that caused by ozone and the expression of a wide-range of genes, including genes encoding important antioxidants, is altered by exposure to ozone. However, little is known about the signal transduction pathways linking the perception of ozone stress to gene expression. We have used a genomics approach to identify novel genes that respond to acute ozone stress in Arabidopsis thaliana. Initial transcript profiling studies using microarray analysis identified twenty genes which were highly up-regulated and one gene that was down-regulated by ozone treatment. Real Time RT-PCR and additional microarray analysis have subsequently confirmed this result. We have analyzed the expression of five of these genes in detail. Our studies show that the induction of all of these genes in response to oxidative stress was dependent on an increase in cytosolic free calcium and was specific to ozone. Importantly, they were not induced by other calcium-mobilizing oxidative stresses including hydrogen peroxide and cold. These data raise important questions about the mechanism by which these genes are induced by ozone and how stimulus specificity is encoded in the ozone calcium signature.

AB - Ozone is an important pollutant that has significant and frequently deleterious effects on plant health resulting in a reduction in cropy ields. This is due largely to oxidative damage caused either directly by ozone or by the reactive oxygen species generated by ozone. Plants possess a range of antioxidant defences to protect them against oxidative stress such as that caused by ozone and the expression of a wide-range of genes, including genes encoding important antioxidants, is altered by exposure to ozone. However, little is known about the signal transduction pathways linking the perception of ozone stress to gene expression. We have used a genomics approach to identify novel genes that respond to acute ozone stress in Arabidopsis thaliana. Initial transcript profiling studies using microarray analysis identified twenty genes which were highly up-regulated and one gene that was down-regulated by ozone treatment. Real Time RT-PCR and additional microarray analysis have subsequently confirmed this result. We have analyzed the expression of five of these genes in detail. Our studies show that the induction of all of these genes in response to oxidative stress was dependent on an increase in cytosolic free calcium and was specific to ozone. Importantly, they were not induced by other calcium-mobilizing oxidative stresses including hydrogen peroxide and cold. These data raise important questions about the mechanism by which these genes are induced by ozone and how stimulus specificity is encoded in the ozone calcium signature.

KW - Arabidopsis

KW - Ozone

KW - Microarray

KW - Calcium

KW - Signalling specificity

M3 - Journal article

VL - 141

SP - S273-S274

JO - Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology

JF - Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology

SN - 1095-6433

IS - 3 Supp

ER -