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Functionalization and immobilization of whole cell bioreporters for the detection of environmental contamination

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Functionalization and immobilization of whole cell bioreporters for the detection of environmental contamination. / Chen, Cheng; Zhang, Dayi; Thornton, Steven F. et al.
In: Environmental Engineering and Management Journal, Vol. 12, No. 7, 01.07.2013, p. 1417-1422.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Chen, C, Zhang, D, Thornton, SF, Duan, M, Luo, Y, Ding, A & Huang, WE 2013, 'Functionalization and immobilization of whole cell bioreporters for the detection of environmental contamination', Environmental Engineering and Management Journal, vol. 12, no. 7, pp. 1417-1422. <http://omicron.ch.tuiasi.ro/EEMJ/pdfs/vol12/no7/15_982_Chen_12.pdf>

APA

Chen, C., Zhang, D., Thornton, S. F., Duan, M., Luo, Y., Ding, A., & Huang, W. E. (2013). Functionalization and immobilization of whole cell bioreporters for the detection of environmental contamination. Environmental Engineering and Management Journal, 12(7), 1417-1422. http://omicron.ch.tuiasi.ro/EEMJ/pdfs/vol12/no7/15_982_Chen_12.pdf

Vancouver

Chen C, Zhang D, Thornton SF, Duan M, Luo Y, Ding A et al. Functionalization and immobilization of whole cell bioreporters for the detection of environmental contamination. Environmental Engineering and Management Journal. 2013 Jul 1;12(7):1417-1422.

Author

Chen, Cheng ; Zhang, Dayi ; Thornton, Steven F. et al. / Functionalization and immobilization of whole cell bioreporters for the detection of environmental contamination. In: Environmental Engineering and Management Journal. 2013 ; Vol. 12, No. 7. pp. 1417-1422.

Bibtex

@article{2f18887c6dc14b0da7b767571804931d,
title = "Functionalization and immobilization of whole cell bioreporters for the detection of environmental contamination",
abstract = "The detection of environmental pollutants has challenged conventional chemical analysis for the rapid estimation of the bioavailability and toxicity of the contamination impact on human health and ecosystems. Magnetic nanoparticles (MNPs) functionalization as bioreporters can enhance the ability for biosensing in complex contaminated samples such as soils, and the immobilization of bioreporters will promote the detection of gas and online monitoring. In this study, Acinetobacter baylyi ADP1 bioreporters ADPWHlux and ADPWHrecA were used to evaluate the optimization of MNPs bioreporter functionalization and filter immobilization. It demonstrated that the MNPs functionalized and filter-immobilized bioreporters retained a similar sensitivity to the original non-treated bioreporters. These approaches will provide new ways for the detection of contaminants in soil and air samples, with prospects for in situ and online measurement. This study also suggested that portable application and miniaturization is possible when the functionalized bioreporter is immobilized on a filter or magnetic probe.",
keywords = "Acinetobacter baylyi ADP1, bioreporter, filter immobilization, genotoxicity, magnetic nanoparticles",
author = "Cheng Chen and Dayi Zhang and Thornton, {Steven F.} and Musen Duan and Yanjun Luo and Aizhong Ding and Huang, {Wei E.}",
year = "2013",
month = jul,
day = "1",
language = "English",
volume = "12",
pages = "1417--1422",
journal = "Environmental Engineering and Management Journal",
issn = "1582-9596",
publisher = "Gh. Asachi Technical University of Iasi",
number = "7",

}

RIS

TY - JOUR

T1 - Functionalization and immobilization of whole cell bioreporters for the detection of environmental contamination

AU - Chen, Cheng

AU - Zhang, Dayi

AU - Thornton, Steven F.

AU - Duan, Musen

AU - Luo, Yanjun

AU - Ding, Aizhong

AU - Huang, Wei E.

PY - 2013/7/1

Y1 - 2013/7/1

N2 - The detection of environmental pollutants has challenged conventional chemical analysis for the rapid estimation of the bioavailability and toxicity of the contamination impact on human health and ecosystems. Magnetic nanoparticles (MNPs) functionalization as bioreporters can enhance the ability for biosensing in complex contaminated samples such as soils, and the immobilization of bioreporters will promote the detection of gas and online monitoring. In this study, Acinetobacter baylyi ADP1 bioreporters ADPWHlux and ADPWHrecA were used to evaluate the optimization of MNPs bioreporter functionalization and filter immobilization. It demonstrated that the MNPs functionalized and filter-immobilized bioreporters retained a similar sensitivity to the original non-treated bioreporters. These approaches will provide new ways for the detection of contaminants in soil and air samples, with prospects for in situ and online measurement. This study also suggested that portable application and miniaturization is possible when the functionalized bioreporter is immobilized on a filter or magnetic probe.

AB - The detection of environmental pollutants has challenged conventional chemical analysis for the rapid estimation of the bioavailability and toxicity of the contamination impact on human health and ecosystems. Magnetic nanoparticles (MNPs) functionalization as bioreporters can enhance the ability for biosensing in complex contaminated samples such as soils, and the immobilization of bioreporters will promote the detection of gas and online monitoring. In this study, Acinetobacter baylyi ADP1 bioreporters ADPWHlux and ADPWHrecA were used to evaluate the optimization of MNPs bioreporter functionalization and filter immobilization. It demonstrated that the MNPs functionalized and filter-immobilized bioreporters retained a similar sensitivity to the original non-treated bioreporters. These approaches will provide new ways for the detection of contaminants in soil and air samples, with prospects for in situ and online measurement. This study also suggested that portable application and miniaturization is possible when the functionalized bioreporter is immobilized on a filter or magnetic probe.

KW - Acinetobacter baylyi ADP1

KW - bioreporter

KW - filter immobilization

KW - genotoxicity

KW - magnetic nanoparticles

M3 - Journal article

VL - 12

SP - 1417

EP - 1422

JO - Environmental Engineering and Management Journal

JF - Environmental Engineering and Management Journal

SN - 1582-9596

IS - 7

ER -