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Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.)

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Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). / Bernard, Stéphanie M; Møller, Anders Laurell Blom; Dionisio, Giuseppe et al.
In: Plant Molecular Biology, Vol. 67, No. 1-2, 2008, p. 89-105.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Bernard, SM, Møller, ALB, Dionisio, G, Kichey, T, Jahn, TP, Dubois, F, Baudo, M, Lopes, MS, Tercé-Laforgue, T, Foyer, CH, Parry, MAJ, Forde, BG, Araus, JL, Hirel, B, Schjoerring, JK & Habash, DZ 2008, 'Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.)', Plant Molecular Biology, vol. 67, no. 1-2, pp. 89-105. https://doi.org/10.1007/s11103-008-9303-y

APA

Bernard, S. M., Møller, A. L. B., Dionisio, G., Kichey, T., Jahn, T. P., Dubois, F., Baudo, M., Lopes, M. S., Tercé-Laforgue, T., Foyer, C. H., Parry, M. A. J., Forde, B. G., Araus, J. L., Hirel, B., Schjoerring, J. K., & Habash, D. Z. (2008). Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). Plant Molecular Biology, 67(1-2), 89-105. https://doi.org/10.1007/s11103-008-9303-y

Vancouver

Bernard SM, Møller ALB, Dionisio G, Kichey T, Jahn TP, Dubois F et al. Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). Plant Molecular Biology. 2008;67(1-2):89-105. doi: 10.1007/s11103-008-9303-y

Author

Bernard, Stéphanie M ; Møller, Anders Laurell Blom ; Dionisio, Giuseppe et al. / Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). In: Plant Molecular Biology. 2008 ; Vol. 67, No. 1-2. pp. 89-105.

Bibtex

@article{a0979c27c26841ab8b4056b1fd4a3b02,
title = "Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.)",
abstract = "We present the first cloning and study of glutamine synthetase (GS) genes in wheat (Triticum aestivum L.). Based on sequence analysis, phylogenetic studies and mapping data, ten GS sequences were classified into four sub-families: GS2 (a, b and c), GS1 (a, b and c), GSr (1 and 2) and GSe (1 and 2). Phylogenetic analysis showed that the wheat GS sub-families together with the GS genes from other monocotyledonous species form four distinct clades. Immunolocalisation studies in leaves, stems and rachis in plants at flowering showed GS protein to be present in parenchyma, phloem companion and perifascicular sheath cells. In situ localisation confirmed that GS1 transcripts were present in the perifascicular sheath cells whilst those for GSr were confined to the vascular cells. Studies of the expression and protein profiles showed that all GS sub-families were differentially expressed in the leaves, peduncle, glumes and roots. Expression of GS genes in leaves was developmentally regulated, with both GS2 and GS1 assimilating or recycling ammonia in leaves during the period of grain development and filling. During leaf senescence the cytosolic isozymes, GS1 and GSr, were the predominant forms, suggesting major roles in assimilating ammonia during the critical phases of remobilisation of nitrogen to the grain. A preliminary analysis of three different wheat genotypes showed that the ratio of leaf GS2 protein to GS1 protein was variable. Use of this genetic variation should inform future efforts to modulate this enzyme for pre-breeding efforts to improve nitrogen use in wheat.",
keywords = "Glutamine synthetase , Wheat , Senescence , Nitrogen, Gene expression , In situ hybridisation , Immunolocalisation",
author = "Bernard, {St{\'e}phanie M} and M{\o}ller, {Anders Laurell Blom} and Giuseppe Dionisio and Thomas Kichey and Jahn, {Thomas P} and Frederic Dubois and Marcela Baudo and Lopes, {Marta S} and Th{\'e}r{\`e}se Terc{\'e}-Laforgue and Foyer, {Christine H} and Parry, {Martin A J} and Forde, {Brian G} and Araus, {Jose Luis} and Bertrand Hirel and Schjoerring, {Jan K} and Habash, {Dimah Z}",
year = "2008",
doi = "10.1007/s11103-008-9303-y",
language = "English",
volume = "67",
pages = "89--105",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "1-2",

}

RIS

TY - JOUR

T1 - Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.)

AU - Bernard, Stéphanie M

AU - Møller, Anders Laurell Blom

AU - Dionisio, Giuseppe

AU - Kichey, Thomas

AU - Jahn, Thomas P

AU - Dubois, Frederic

AU - Baudo, Marcela

AU - Lopes, Marta S

AU - Tercé-Laforgue, Thérèse

AU - Foyer, Christine H

AU - Parry, Martin A J

AU - Forde, Brian G

AU - Araus, Jose Luis

AU - Hirel, Bertrand

AU - Schjoerring, Jan K

AU - Habash, Dimah Z

PY - 2008

Y1 - 2008

N2 - We present the first cloning and study of glutamine synthetase (GS) genes in wheat (Triticum aestivum L.). Based on sequence analysis, phylogenetic studies and mapping data, ten GS sequences were classified into four sub-families: GS2 (a, b and c), GS1 (a, b and c), GSr (1 and 2) and GSe (1 and 2). Phylogenetic analysis showed that the wheat GS sub-families together with the GS genes from other monocotyledonous species form four distinct clades. Immunolocalisation studies in leaves, stems and rachis in plants at flowering showed GS protein to be present in parenchyma, phloem companion and perifascicular sheath cells. In situ localisation confirmed that GS1 transcripts were present in the perifascicular sheath cells whilst those for GSr were confined to the vascular cells. Studies of the expression and protein profiles showed that all GS sub-families were differentially expressed in the leaves, peduncle, glumes and roots. Expression of GS genes in leaves was developmentally regulated, with both GS2 and GS1 assimilating or recycling ammonia in leaves during the period of grain development and filling. During leaf senescence the cytosolic isozymes, GS1 and GSr, were the predominant forms, suggesting major roles in assimilating ammonia during the critical phases of remobilisation of nitrogen to the grain. A preliminary analysis of three different wheat genotypes showed that the ratio of leaf GS2 protein to GS1 protein was variable. Use of this genetic variation should inform future efforts to modulate this enzyme for pre-breeding efforts to improve nitrogen use in wheat.

AB - We present the first cloning and study of glutamine synthetase (GS) genes in wheat (Triticum aestivum L.). Based on sequence analysis, phylogenetic studies and mapping data, ten GS sequences were classified into four sub-families: GS2 (a, b and c), GS1 (a, b and c), GSr (1 and 2) and GSe (1 and 2). Phylogenetic analysis showed that the wheat GS sub-families together with the GS genes from other monocotyledonous species form four distinct clades. Immunolocalisation studies in leaves, stems and rachis in plants at flowering showed GS protein to be present in parenchyma, phloem companion and perifascicular sheath cells. In situ localisation confirmed that GS1 transcripts were present in the perifascicular sheath cells whilst those for GSr were confined to the vascular cells. Studies of the expression and protein profiles showed that all GS sub-families were differentially expressed in the leaves, peduncle, glumes and roots. Expression of GS genes in leaves was developmentally regulated, with both GS2 and GS1 assimilating or recycling ammonia in leaves during the period of grain development and filling. During leaf senescence the cytosolic isozymes, GS1 and GSr, were the predominant forms, suggesting major roles in assimilating ammonia during the critical phases of remobilisation of nitrogen to the grain. A preliminary analysis of three different wheat genotypes showed that the ratio of leaf GS2 protein to GS1 protein was variable. Use of this genetic variation should inform future efforts to modulate this enzyme for pre-breeding efforts to improve nitrogen use in wheat.

KW - Glutamine synthetase

KW - Wheat

KW - Senescence

KW - Nitrogen

KW - Gene expression

KW - In situ hybridisation

KW - Immunolocalisation

UR - http://www.scopus.com/inward/record.url?scp=42149149802&partnerID=8YFLogxK

U2 - 10.1007/s11103-008-9303-y

DO - 10.1007/s11103-008-9303-y

M3 - Journal article

C2 - 18288574

VL - 67

SP - 89

EP - 105

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 1-2

ER -