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Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means

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Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means. / Douglas, Timothy Edward Lim; Krawczyk, Grzegorz; Pamula, Elzbieta; Declercq, Heidi; Schaubroeck, David; Bucko, Miroslaw; Balcaen, Lieve; Van der Voort, Pascal; Bliznuk, Vitaliy; van den Vreken, Natasja; Dash, Mamoni; Detsch, Rainer; Boccaccini, Aldo; Vanhaecke, Frank; Cornelissen, Ria; Dubruel.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 10, No. 11, 11.2016, p. 938-954.

Research output: Contribution to journalJournal article

Harvard

Douglas, TEL, Krawczyk, G, Pamula, E, Declercq, H, Schaubroeck, D, Bucko, M, Balcaen, L, Van der Voort, P, Bliznuk, V, van den Vreken, N, Dash, M, Detsch, R, Boccaccini, A, Vanhaecke, F, Cornelissen, R & Dubruel 2016, 'Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means', Journal of Tissue Engineering and Regenerative Medicine, vol. 10, no. 11, pp. 938-954. https://doi.org/10.1002/term.1875

APA

Douglas, T. E. L., Krawczyk, G., Pamula, E., Declercq, H., Schaubroeck, D., Bucko, M., ... Dubruel (2016). Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means. Journal of Tissue Engineering and Regenerative Medicine, 10(11), 938-954. https://doi.org/10.1002/term.1875

Vancouver

Douglas TEL, Krawczyk G, Pamula E, Declercq H, Schaubroeck D, Bucko M et al. Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means. Journal of Tissue Engineering and Regenerative Medicine. 2016 Nov;10(11):938-954. https://doi.org/10.1002/term.1875

Author

Douglas, Timothy Edward Lim ; Krawczyk, Grzegorz ; Pamula, Elzbieta ; Declercq, Heidi ; Schaubroeck, David ; Bucko, Miroslaw ; Balcaen, Lieve ; Van der Voort, Pascal ; Bliznuk, Vitaliy ; van den Vreken, Natasja ; Dash, Mamoni ; Detsch, Rainer ; Boccaccini, Aldo ; Vanhaecke, Frank ; Cornelissen, Ria ; Dubruel. / Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means. In: Journal of Tissue Engineering and Regenerative Medicine. 2016 ; Vol. 10, No. 11. pp. 938-954.

Bibtex

@article{25f512f777f442568c3d32cb714968ce,
title = "Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means",
abstract = "Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP). ALP‐loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP). Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3, denoted A, B, C, D and E, respectively) were compared. Mineral formation was confirmed by IR and Raman, SEM, ICP‐OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water. Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A–D. Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium‐deficient hydroxyapatite (CDHA). Mineral formed in medium C was a combination of CDHA and an amorphous phase. Mineral formed in medium D was an amorphous phase. Mineral formed in medium E was a combination of crystalline and amorphous MgP. Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E. The attachment and vitality of osteoblastic MC3T3‐E1 cells were higher on samples mineralized in media B–E (containing Mg) than in those mineralized in medium A (not containing Mg). All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast‐like cell formation.",
keywords = "hydrogel, composite, calcium phosphate, magnesium phosphate, enzyme, gellan gum, osteoblast, cytocompatibility",
author = "Douglas, {Timothy Edward Lim} and Grzegorz Krawczyk and Elzbieta Pamula and Heidi Declercq and David Schaubroeck and Miroslaw Bucko and Lieve Balcaen and {Van der Voort}, Pascal and Vitaliy Bliznuk and {van den Vreken}, Natasja and Mamoni Dash and Rainer Detsch and Aldo Boccaccini and Frank Vanhaecke and Ria Cornelissen and Dubruel",
year = "2016",
month = "11",
doi = "10.1002/term.1875",
language = "English",
volume = "10",
pages = "938--954",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "John Wiley and Sons Ltd",
number = "11",

}

RIS

TY - JOUR

T1 - Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means

AU - Douglas, Timothy Edward Lim

AU - Krawczyk, Grzegorz

AU - Pamula, Elzbieta

AU - Declercq, Heidi

AU - Schaubroeck, David

AU - Bucko, Miroslaw

AU - Balcaen, Lieve

AU - Van der Voort, Pascal

AU - Bliznuk, Vitaliy

AU - van den Vreken, Natasja

AU - Dash, Mamoni

AU - Detsch, Rainer

AU - Boccaccini, Aldo

AU - Vanhaecke, Frank

AU - Cornelissen, Ria

AU - Dubruel,

PY - 2016/11

Y1 - 2016/11

N2 - Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP). ALP‐loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP). Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3, denoted A, B, C, D and E, respectively) were compared. Mineral formation was confirmed by IR and Raman, SEM, ICP‐OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water. Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A–D. Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium‐deficient hydroxyapatite (CDHA). Mineral formed in medium C was a combination of CDHA and an amorphous phase. Mineral formed in medium D was an amorphous phase. Mineral formed in medium E was a combination of crystalline and amorphous MgP. Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E. The attachment and vitality of osteoblastic MC3T3‐E1 cells were higher on samples mineralized in media B–E (containing Mg) than in those mineralized in medium A (not containing Mg). All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast‐like cell formation.

AB - Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP). ALP‐loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP). Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3, denoted A, B, C, D and E, respectively) were compared. Mineral formation was confirmed by IR and Raman, SEM, ICP‐OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water. Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A–D. Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium‐deficient hydroxyapatite (CDHA). Mineral formed in medium C was a combination of CDHA and an amorphous phase. Mineral formed in medium D was an amorphous phase. Mineral formed in medium E was a combination of crystalline and amorphous MgP. Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E. The attachment and vitality of osteoblastic MC3T3‐E1 cells were higher on samples mineralized in media B–E (containing Mg) than in those mineralized in medium A (not containing Mg). All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast‐like cell formation.

KW - hydrogel

KW - composite

KW - calcium phosphate

KW - magnesium phosphate

KW - enzyme

KW - gellan gum

KW - osteoblast

KW - cytocompatibility

U2 - 10.1002/term.1875

DO - 10.1002/term.1875

M3 - Journal article

VL - 10

SP - 938

EP - 954

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

IS - 11

ER -