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Genetic control of lysine metabolism in maize endosperm mutants.

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Genetic control of lysine metabolism in maize endosperm mutants. / Azevedo, Ricardo A.; Damerval, Catherine; Lea, Peter J. et al.
In: Functional Plant Biology, Vol. 31, No. 4, 2004, p. 339-348.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Azevedo, RA, Damerval, C, Lea, PJ, Landry, J, Bellato, CM, Meinhardt, LW, Le Guilloux, M, Delhaye, S, Toro, AA, Gaziola, SA, Varisi, VA & Gratão, PL 2004, 'Genetic control of lysine metabolism in maize endosperm mutants.', Functional Plant Biology, vol. 31, no. 4, pp. 339-348. https://doi.org/10.1071/FP03173

APA

Azevedo, R. A., Damerval, C., Lea, P. J., Landry, J., Bellato, C. M., Meinhardt, L. W., Le Guilloux, M., Delhaye, S., Toro, A. A., Gaziola, S. A., Varisi, V. A., & Gratão, P. L. (2004). Genetic control of lysine metabolism in maize endosperm mutants. Functional Plant Biology, 31(4), 339-348. https://doi.org/10.1071/FP03173

Vancouver

Azevedo RA, Damerval C, Lea PJ, Landry J, Bellato CM, Meinhardt LW et al. Genetic control of lysine metabolism in maize endosperm mutants. Functional Plant Biology. 2004;31(4):339-348. doi: 10.1071/FP03173

Author

Azevedo, Ricardo A. ; Damerval, Catherine ; Lea, Peter J. et al. / Genetic control of lysine metabolism in maize endosperm mutants. In: Functional Plant Biology. 2004 ; Vol. 31, No. 4. pp. 339-348.

Bibtex

@article{937a61db76444bddbac97bdef05d0047,
title = "Genetic control of lysine metabolism in maize endosperm mutants.",
abstract = "The capacity of three maize endosperm opaque mutants (o10, o11 and o13) to accumulate soluble lysine in the seed in relation to their wildtype counterpart, W22+, was investigated. The W22o13 and W22o11 mutants exhibited 278% and 186% increases in soluble lysine, respectively, while for W22o10, a 36% decrease was observed, compared with the wildtype. A quantitative and qualitative study of the N constituents of the endosperm has been conducted and data obtained for the total protein, non-protein N, soluble amino acids, albumins / globulins, zeins and glutelins present in the seed of the mutants. Following 2D–PAGE, a total of 38 different forms of zein polypeptides were detected and considerable differences were noted between the three mutant lines. The metabolism of lysine was also studied by analysis of the enzymes aspartate kinase, homoserine dehydrogenase, lysine 2-oxoglutarate reductase and saccharopine dehydrogenase, which exhibited major changes in activity, depending on the genotype, suggesting that the mutant genes may have distinct regulatory activities.",
keywords = "aspartate kinase, lysine 2-oxoglutarate reductase, maize, mutants, storage proteins.",
author = "Azevedo, {Ricardo A.} and Catherine Damerval and Lea, {Peter J.} and Jacques Landry and Bellato, {Cl{\'a}udia M.} and Meinhardt, {Lyndel W.} and {Le Guilloux}, Martine and Sonia Delhaye and Toro, {Alejandro A.} and Gaziola, {Salete A.} and Varisi, {Vanderlei A.} and Grat{\~a}o, {Priscila Lupino}",
year = "2004",
doi = "10.1071/FP03173",
language = "English",
volume = "31",
pages = "339--348",
journal = "Functional Plant Biology",
issn = "1445-4408",
publisher = "CSIRO PUBLISHING",
number = "4",

}

RIS

TY - JOUR

T1 - Genetic control of lysine metabolism in maize endosperm mutants.

AU - Azevedo, Ricardo A.

AU - Damerval, Catherine

AU - Lea, Peter J.

AU - Landry, Jacques

AU - Bellato, Cláudia M.

AU - Meinhardt, Lyndel W.

AU - Le Guilloux, Martine

AU - Delhaye, Sonia

AU - Toro, Alejandro A.

AU - Gaziola, Salete A.

AU - Varisi, Vanderlei A.

AU - Gratão, Priscila Lupino

PY - 2004

Y1 - 2004

N2 - The capacity of three maize endosperm opaque mutants (o10, o11 and o13) to accumulate soluble lysine in the seed in relation to their wildtype counterpart, W22+, was investigated. The W22o13 and W22o11 mutants exhibited 278% and 186% increases in soluble lysine, respectively, while for W22o10, a 36% decrease was observed, compared with the wildtype. A quantitative and qualitative study of the N constituents of the endosperm has been conducted and data obtained for the total protein, non-protein N, soluble amino acids, albumins / globulins, zeins and glutelins present in the seed of the mutants. Following 2D–PAGE, a total of 38 different forms of zein polypeptides were detected and considerable differences were noted between the three mutant lines. The metabolism of lysine was also studied by analysis of the enzymes aspartate kinase, homoserine dehydrogenase, lysine 2-oxoglutarate reductase and saccharopine dehydrogenase, which exhibited major changes in activity, depending on the genotype, suggesting that the mutant genes may have distinct regulatory activities.

AB - The capacity of three maize endosperm opaque mutants (o10, o11 and o13) to accumulate soluble lysine in the seed in relation to their wildtype counterpart, W22+, was investigated. The W22o13 and W22o11 mutants exhibited 278% and 186% increases in soluble lysine, respectively, while for W22o10, a 36% decrease was observed, compared with the wildtype. A quantitative and qualitative study of the N constituents of the endosperm has been conducted and data obtained for the total protein, non-protein N, soluble amino acids, albumins / globulins, zeins and glutelins present in the seed of the mutants. Following 2D–PAGE, a total of 38 different forms of zein polypeptides were detected and considerable differences were noted between the three mutant lines. The metabolism of lysine was also studied by analysis of the enzymes aspartate kinase, homoserine dehydrogenase, lysine 2-oxoglutarate reductase and saccharopine dehydrogenase, which exhibited major changes in activity, depending on the genotype, suggesting that the mutant genes may have distinct regulatory activities.

KW - aspartate kinase

KW - lysine 2-oxoglutarate reductase

KW - maize

KW - mutants

KW - storage proteins.

U2 - 10.1071/FP03173

DO - 10.1071/FP03173

M3 - Journal article

VL - 31

SP - 339

EP - 348

JO - Functional Plant Biology

JF - Functional Plant Biology

SN - 1445-4408

IS - 4

ER -