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Genetic manipulation of Rubisco: chromatium vinosum rbcL is expressed in Nicotiana tabacum but does not form a functional protein

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published
  • Pippa J. Madgwick
  • S. P. Colliver
  • F. M. Banks
  • D. Z. Habash
  • H. Dulieu
  • M. A J Parry
  • M. J. Paul
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<mark>Journal publication date</mark>18/04/2002
<mark>Journal</mark>Annals of Applied Biology
Issue number1
Volume140
Number of pages7
Pages (from-to)13-19
Publication StatusPublished
<mark>Original language</mark>English

Abstract

N. tabacum lines that lacked functional Rubisco were transformed with plasmids encoding a chloroplast transit peptide in frame with C. vinosum rbcL and stable transformants generated. However, the transgene was transcribed at a low level and no Rubisco activity or C. vinosum large subunits were detectable in any line.