We tested the effects of ungulate grazing and nutrient availability on the temperature sensitivity of soil respiration (CO₂) and methane (CH₄) emissions in semi-natural temperate grassland. To do this, soil taken from long term grazed and ungrazed grassland was incubated at four temperatures (4, 10, 15 and 20°C) with two levels of nutrient (NP) addition. The results showed that the variation in soil CO₂ and CH₄ emissions was explained by temperature and grazing, with grazing increasing the temperature sensitivity of CO₂ and CH₄ production by between 15 and 20°C. This response was constrained by nutrient availability for CO₂, but not CH₄. These findings suggest that grazing could potentially have important impacts on the temperature sensitivity of greenhouse gas emissions in nutrient limited grasslands.