Growth inhibition of the filamentous fungus Aspergillus nidulans by cadmium: an antioxidant enzyme approach.

Lancaster Environment Centre

Keywords

Aspergillus nidulans, catalase, glutathione reductase, oxidative stress, superoxide dismutase

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Growth inhibition of the filamentous fungus Aspergillus nidulans by cadmium: an antioxidant enzyme approach. / Guelfi, A.; Azevedo, R. A.; Lea, P. J. et al.
In: Journal of General and Applied Microbiology, Vol. 49, No. 2, 04.2003, p. 63-73.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Author

Guelfi, A. ; Azevedo, R. A. ; Lea, P. J. et al. / Growth inhibition of the filamentous fungus Aspergillus nidulans by cadmium: an antioxidant enzyme approach. In: Journal of General and Applied Microbiology. 2003 ; Vol. 49, No. 2. pp. 63-73.

Bibtex

@article{4c95d9be725b46e1af6e9e2023f7b249,

title = "Growth inhibition of the filamentous fungus Aspergillus nidulans by cadmium: an antioxidant enzyme approach.",

abstract = "The heavy metal cadmium is very toxic to biological systems. Although its effect on the growth of microorganisms and plants has been investigated, the response of antioxidant enzymes of Aspergillus nidulans to cadmium is not well documented. We have studied the effect of cadmium (supplied as CdCl2) on catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR). 0.005 mm CdCl2 had a very slight stimulatory effect on the growth rate of A. nidulans, but at concentrations above 0.025 mm, growth was totally inhibited. The accumulation of Cd within the mycelium was directly correlated with the increase in the concentration of CdCl2 used in the treatments. Although a cadmium-stimulated increase in SOD activity was observed, there was no change in the relative proportions of the individual MnSOD isoenzymes. Higher concentrations of CdCl2 induced a small increase in total CAT activity, but there was a major increase in one isoenzymic form, that could be separated by gel electrophoresis. GR activity increased significantly following treatment with the highest concentration (0.05 mm) of CdCl2. The increases in SOD, CAT, and GR activities suggest that CdCl2 induces the formation of reactive oxygen species inside the mycelia of A. nidulans.",

keywords = "Aspergillus nidulans, catalase, glutathione reductase, oxidative stress, superoxide dismutase",

author = "A. Guelfi and Azevedo, {R. A.} and Lea, {P. J.} and Molina, {S. M. G.}",

year = "2003",

month = apr,

language = "English",

volume = "49",

pages = "63--73",

journal = "Journal of General and Applied Microbiology",

issn = "0022-1260",

publisher = "Microbiology Research Foundation",

number = "2",

}

RIS

TY - JOUR

T1 - Growth inhibition of the filamentous fungus Aspergillus nidulans by cadmium: an antioxidant enzyme approach.

AU - Guelfi, A.

AU - Azevedo, R. A.

AU - Lea, P. J.

AU - Molina, S. M. G.

PY - 2003/4

Y1 - 2003/4

N2 - The heavy metal cadmium is very toxic to biological systems. Although its effect on the growth of microorganisms and plants has been investigated, the response of antioxidant enzymes of Aspergillus nidulans to cadmium is not well documented. We have studied the effect of cadmium (supplied as CdCl2) on catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR). 0.005 mm CdCl2 had a very slight stimulatory effect on the growth rate of A. nidulans, but at concentrations above 0.025 mm, growth was totally inhibited. The accumulation of Cd within the mycelium was directly correlated with the increase in the concentration of CdCl2 used in the treatments. Although a cadmium-stimulated increase in SOD activity was observed, there was no change in the relative proportions of the individual MnSOD isoenzymes. Higher concentrations of CdCl2 induced a small increase in total CAT activity, but there was a major increase in one isoenzymic form, that could be separated by gel electrophoresis. GR activity increased significantly following treatment with the highest concentration (0.05 mm) of CdCl2. The increases in SOD, CAT, and GR activities suggest that CdCl2 induces the formation of reactive oxygen species inside the mycelia of A. nidulans.

AB - The heavy metal cadmium is very toxic to biological systems. Although its effect on the growth of microorganisms and plants has been investigated, the response of antioxidant enzymes of Aspergillus nidulans to cadmium is not well documented. We have studied the effect of cadmium (supplied as CdCl2) on catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR). 0.005 mm CdCl2 had a very slight stimulatory effect on the growth rate of A. nidulans, but at concentrations above 0.025 mm, growth was totally inhibited. The accumulation of Cd within the mycelium was directly correlated with the increase in the concentration of CdCl2 used in the treatments. Although a cadmium-stimulated increase in SOD activity was observed, there was no change in the relative proportions of the individual MnSOD isoenzymes. Higher concentrations of CdCl2 induced a small increase in total CAT activity, but there was a major increase in one isoenzymic form, that could be separated by gel electrophoresis. GR activity increased significantly following treatment with the highest concentration (0.05 mm) of CdCl2. The increases in SOD, CAT, and GR activities suggest that CdCl2 induces the formation of reactive oxygen species inside the mycelia of A. nidulans.

KW - Aspergillus nidulans

KW - catalase

KW - glutathione reductase

KW - oxidative stress

KW - superoxide dismutase

M3 - Journal article

VL - 49

SP - 63

EP - 73

JO - Journal of General and Applied Microbiology

JF - Journal of General and Applied Microbiology

SN - 0022-1260

IS - 2

ER -

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