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  • Guther et al JPR 2014 pr401209w

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High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics

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<mark>Journal publication date</mark>2014
<mark>Journal</mark>Journal of Proteome Research
Issue number6
Volume13
Number of pages11
Pages (from-to)2796-2806
<mark>State</mark>Published
Early online date13/05/14
<mark>Original language</mark>English

Abstract

The glycosome of the pathogenic African trypanosome Trypanosoma brucei is a specialized peroxisome that contains most of the enzymes of glycolysis and several other metabolic and catabolic pathways. The contents and transporters of this membrane-bounded organelle are of considerable interest as potential drug targets. Here we use epitope tagging, magnetic bead enrichment, and SILAC quantitative proteomics to determine a high-confidence glycosome proteome for the procyclic life cycle stage of the parasite using isotope ratios to discriminate glycosomal from mitochondrial and other contaminating proteins. The data confirm the presence of several previously demonstrated and suggested pathways in the organelle and identify previously unanticipated activities, such as protein phosphatases. The implications of the findings are discussed.

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ACS AuthorChoice/Editors’ Choice via Creative Commons CC-BY Usage Agreement