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  • Guther et al JPR 2014 pr401209w

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High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics. / Güther, Maria Lucia S.; Urbaniak, Michael D.; Tavendale, Amy et al.
In: Journal of Proteome Research, Vol. 13, No. 6, 2014, p. 2796-2806.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Güther, MLS, Urbaniak, MD, Tavendale, A, Prescott, A & Ferguson, MAJ 2014, 'High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics', Journal of Proteome Research, vol. 13, no. 6, pp. 2796-2806. https://doi.org/10.1021/pr401209w

APA

Güther, M. L. S., Urbaniak, M. D., Tavendale, A., Prescott, A., & Ferguson, M. A. J. (2014). High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics. Journal of Proteome Research, 13(6), 2796-2806. https://doi.org/10.1021/pr401209w

Vancouver

Güther MLS, Urbaniak MD, Tavendale A, Prescott A, Ferguson MAJ. High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics. Journal of Proteome Research. 2014;13(6):2796-2806. Epub 2014 May 13. doi: 10.1021/pr401209w

Author

Güther, Maria Lucia S. ; Urbaniak, Michael D. ; Tavendale, Amy et al. / High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics. In: Journal of Proteome Research. 2014 ; Vol. 13, No. 6. pp. 2796-2806.

Bibtex

@article{6d3b49b64dcc48b8ae939df1c61e3046,
title = "High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics",
abstract = "The glycosome of the pathogenic African trypanosome Trypanosoma brucei is a specialized peroxisome that contains most of the enzymes of glycolysis and several other metabolic and catabolic pathways. The contents and transporters of this membrane-bounded organelle are of considerable interest as potential drug targets. Here we use epitope tagging, magnetic bead enrichment, and SILAC quantitative proteomics to determine a high-confidence glycosome proteome for the procyclic life cycle stage of the parasite using isotope ratios to discriminate glycosomal from mitochondrial and other contaminating proteins. The data confirm the presence of several previously demonstrated and suggested pathways in the organelle and identify previously unanticipated activities, such as protein phosphatases. The implications of the findings are discussed.",
author = "G{\"u}ther, {Maria Lucia S.} and Urbaniak, {Michael D.} and Amy Tavendale and Alan Prescott and Ferguson, {Michael A. J.}",
note = "ACS AuthorChoice/Editors{\textquoteright} Choice via Creative Commons CC-BY Usage Agreement",
year = "2014",
doi = "10.1021/pr401209w",
language = "English",
volume = "13",
pages = "2796--2806",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "6",

}

RIS

TY - JOUR

T1 - High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics

AU - Güther, Maria Lucia S.

AU - Urbaniak, Michael D.

AU - Tavendale, Amy

AU - Prescott, Alan

AU - Ferguson, Michael A. J.

N1 - ACS AuthorChoice/Editors’ Choice via Creative Commons CC-BY Usage Agreement

PY - 2014

Y1 - 2014

N2 - The glycosome of the pathogenic African trypanosome Trypanosoma brucei is a specialized peroxisome that contains most of the enzymes of glycolysis and several other metabolic and catabolic pathways. The contents and transporters of this membrane-bounded organelle are of considerable interest as potential drug targets. Here we use epitope tagging, magnetic bead enrichment, and SILAC quantitative proteomics to determine a high-confidence glycosome proteome for the procyclic life cycle stage of the parasite using isotope ratios to discriminate glycosomal from mitochondrial and other contaminating proteins. The data confirm the presence of several previously demonstrated and suggested pathways in the organelle and identify previously unanticipated activities, such as protein phosphatases. The implications of the findings are discussed.

AB - The glycosome of the pathogenic African trypanosome Trypanosoma brucei is a specialized peroxisome that contains most of the enzymes of glycolysis and several other metabolic and catabolic pathways. The contents and transporters of this membrane-bounded organelle are of considerable interest as potential drug targets. Here we use epitope tagging, magnetic bead enrichment, and SILAC quantitative proteomics to determine a high-confidence glycosome proteome for the procyclic life cycle stage of the parasite using isotope ratios to discriminate glycosomal from mitochondrial and other contaminating proteins. The data confirm the presence of several previously demonstrated and suggested pathways in the organelle and identify previously unanticipated activities, such as protein phosphatases. The implications of the findings are discussed.

U2 - 10.1021/pr401209w

DO - 10.1021/pr401209w

M3 - Journal article

C2 - 24792668

VL - 13

SP - 2796

EP - 2806

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 6

ER -