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High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169

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High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169. / Bradley, Amanda J.; Lurain, Nell S.; Ghazal, Peter; Trivedi, Urmi; Cunningham, Charles; Baluchova, Katarina; Gatherer, Derek; Wilkinson, Gavin W. G.; Dargan, Derrick J.; Davison, Andrew J.

In: Journal of General Virology, Vol. 90, No. 10, 10.2009, p. 2375-2380.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Bradley, AJ, Lurain, NS, Ghazal, P, Trivedi, U, Cunningham, C, Baluchova, K, Gatherer, D, Wilkinson, GWG, Dargan, DJ & Davison, AJ 2009, 'High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169', Journal of General Virology, vol. 90, no. 10, pp. 2375-2380. https://doi.org/10.1099/vir.0.013250-0

APA

Bradley, A. J., Lurain, N. S., Ghazal, P., Trivedi, U., Cunningham, C., Baluchova, K., Gatherer, D., Wilkinson, G. W. G., Dargan, D. J., & Davison, A. J. (2009). High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169. Journal of General Virology, 90(10), 2375-2380. https://doi.org/10.1099/vir.0.013250-0

Vancouver

Bradley AJ, Lurain NS, Ghazal P, Trivedi U, Cunningham C, Baluchova K et al. High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169. Journal of General Virology. 2009 Oct;90(10):2375-2380. https://doi.org/10.1099/vir.0.013250-0

Author

Bradley, Amanda J. ; Lurain, Nell S. ; Ghazal, Peter ; Trivedi, Urmi ; Cunningham, Charles ; Baluchova, Katarina ; Gatherer, Derek ; Wilkinson, Gavin W. G. ; Dargan, Derrick J. ; Davison, Andrew J. / High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169. In: Journal of General Virology. 2009 ; Vol. 90, No. 10. pp. 2375-2380.

Bibtex

@article{1c791504fedd48c2ab7f62c0ef923b2c,
title = "High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169",
abstract = "The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.",
keywords = "Base Sequence, Cytomegalovirus, DNA, Viral, Genetic Variation, Genome, Viral, Humans, Mutation",
author = "Bradley, {Amanda J.} and Lurain, {Nell S.} and Peter Ghazal and Urmi Trivedi and Charles Cunningham and Katarina Baluchova and Derek Gatherer and Wilkinson, {Gavin W. G.} and Dargan, {Derrick J.} and Davison, {Andrew J.}",
year = "2009",
month = oct,
doi = "10.1099/vir.0.013250-0",
language = "English",
volume = "90",
pages = "2375--2380",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169

AU - Bradley, Amanda J.

AU - Lurain, Nell S.

AU - Ghazal, Peter

AU - Trivedi, Urmi

AU - Cunningham, Charles

AU - Baluchova, Katarina

AU - Gatherer, Derek

AU - Wilkinson, Gavin W. G.

AU - Dargan, Derrick J.

AU - Davison, Andrew J.

PY - 2009/10

Y1 - 2009/10

N2 - The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.

AB - The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.

KW - Base Sequence

KW - Cytomegalovirus

KW - DNA, Viral

KW - Genetic Variation

KW - Genome, Viral

KW - Humans

KW - Mutation

U2 - 10.1099/vir.0.013250-0

DO - 10.1099/vir.0.013250-0

M3 - Journal article

C2 - 19553388

VL - 90

SP - 2375

EP - 2380

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 10

ER -