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  • ytterbium - DMPC bilayers paper

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How does ytterbium chloride interact with DMPC bilayers?: A computational and experimental study

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  • Miguel A. Gonzalez
  • Hanna M.G. Barriga
  • Joanna L. Richens
  • Robert V. Law
  • Paul O'Shea
  • Fernando Bresme
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<mark>Journal publication date</mark>7/04/2017
<mark>Journal</mark>Physical Chemistry Chemical Physics
Issue number13
Volume19
Number of pages11
Pages (from-to)9199-9209
Publication statusPublished
Early online date20/03/17
Original languageEnglish

Abstract

Lanthanide salts have been studied for many years, primarily in NMR experiments of mixed lipid-protein systems and more recently to study lipid flip-flop in model membrane systems. It is well recognised that lanthanide salts can influence the behaviour of both lipid and protein systems, however a full molecular level description of lipid-lanthanide interactions is still outstanding. Here we present a study of lanthanide-bilayer interactions, using molecular dynamics computer simu-lations, fluorescence electrostatic potential experiments and Nuclear Magnetic Resonance. Com-puter simulations reveal the microscopic structure of DMPC lipid bilayers in the presence of Yb 3+ , and a surprising ability of the membranes to adsorb significant concentrations of Yb 3+ without significant disruption of the overall membrane structure. The Yb 3+ ions bind strongly to the lipids via the oxygen atoms in the lipid head group. We find that the cations are coordinated to 4-5 lipids for a wide range of Lanthanide:lipid ratios and temperatures. Addition of Yb 3+ results in a small decrease of the area per lipid with a concomitant increase of the ordering of the aliphatic chains and the bilayer thickness. The addition of Yb 3+ at standard concentrations commonly used in the NMR, induces an increase of the membrane electrostatic potential, ∼ 110 mV and a large change in the head-group orientation, which aligns in the direction normal to the bilayer plane. In addition the area compressibility modulus (stiffness) of DMPC having Ytterbium salt is 2.6 time higher than the membrane free-salt. These changes in the membrane properties are enhanced with salt con-centration, and should be taken into account in the interpretation of NMR experiments performed with Lanthanides.