Hoxa2 selectively enhances meis binding to change a branchial arch ground state

Data Science Institute

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https://doi.org/10.1016/j.devcel.2014.12.024
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Hoxa2 selectively enhances meis binding to change a branchial arch ground state. / Amin, Shilu; Donaldson, Ian J.; Zannino, Denise A. et al.
In: Developmental Cell, Vol. 32, No. 3, 09.02.2015, p. 265-277.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Amin, S, Donaldson, IJ, Zannino, DA, Hensman, J, Rattray, M, Losa, M, Spitz, F, Ladam, F, Sagerström, C & Bobola, N 2015, 'Hoxa2 selectively enhances meis binding to change a branchial arch ground state', Developmental Cell, vol. 32, no. 3, pp. 265-277. https://doi.org/10.1016/j.devcel.2014.12.024

APA

Amin, S., Donaldson, I. J., Zannino, D. A., Hensman, J., Rattray, M., Losa, M., Spitz, F., Ladam, F., Sagerström, C., & Bobola, N. (2015). Hoxa2 selectively enhances meis binding to change a branchial arch ground state. Developmental Cell, 32(3), 265-277. https://doi.org/10.1016/j.devcel.2014.12.024

Vancouver

Amin S, Donaldson IJ, Zannino DA, Hensman J, Rattray M, Losa M et al. Hoxa2 selectively enhances meis binding to change a branchial arch ground state. Developmental Cell. 2015 Feb 9;32(3):265-277. Epub 2015 Jan 29. doi: 10.1016/j.devcel.2014.12.024

Author

Amin, Shilu ; Donaldson, Ian J. ; Zannino, Denise A. et al. / Hoxa2 selectively enhances meis binding to change a branchial arch ground state. In: Developmental Cell. 2015 ; Vol. 32, No. 3. pp. 265-277.

Bibtex

@article{7a4bd77c58674c5b84eef6ff2728394a,

title = "Hoxa2 selectively enhances meis binding to change a branchial arch ground state",

abstract = "Hox transcription factors (TFs) are essential for vertebrate development, but how these evolutionary conserved proteins function invivo remains unclear. Because Hox proteins have notoriously low binding specificity, they are believed to bind with cofactors, mainly homeodomain TFs Pbx and Meis, to select their specific targets. We mapped binding of Meis, Pbx, and Hoxa2 in the branchial arches, a series of segments in the developing vertebrate head. Meis occupancy is largely similar in Hox-positive and -negative arches. Hoxa2, which specifies second arch (IIBA) identity, recognizes a subset of Meis preboundsites that contain Hox motifs. Importantly, at these sites Meis binding is strongly increased. This enhanced Meis binding coincides with active enhancers, which are linked to genes highly expressed in the IIBA and regulated by Hoxa2. These findings show that Hoxa2 operates as a tissue-specific cofactor, enhancing Meis binding to specific sites that provide the IIBA with its anatomical identity.",

author = "Shilu Amin and Donaldson, {Ian J.} and Zannino, {Denise A.} and James Hensman and Magnus Rattray and Marta Losa and Fran{\c c}ois Spitz and Franck Ladam and Charles Sagerstr{\"o}m and Nicoletta Bobola",

year = "2015",

month = feb,

day = "9",

doi = "10.1016/j.devcel.2014.12.024",

language = "English",

volume = "32",

pages = "265--277",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Cell Press",

number = "3",

}

RIS

TY - JOUR

T1 - Hoxa2 selectively enhances meis binding to change a branchial arch ground state

AU - Amin, Shilu

AU - Donaldson, Ian J.

AU - Zannino, Denise A.

AU - Hensman, James

AU - Rattray, Magnus

AU - Losa, Marta

AU - Spitz, François

AU - Ladam, Franck

AU - Sagerström, Charles

AU - Bobola, Nicoletta

PY - 2015/2/9

Y1 - 2015/2/9

N2 - Hox transcription factors (TFs) are essential for vertebrate development, but how these evolutionary conserved proteins function invivo remains unclear. Because Hox proteins have notoriously low binding specificity, they are believed to bind with cofactors, mainly homeodomain TFs Pbx and Meis, to select their specific targets. We mapped binding of Meis, Pbx, and Hoxa2 in the branchial arches, a series of segments in the developing vertebrate head. Meis occupancy is largely similar in Hox-positive and -negative arches. Hoxa2, which specifies second arch (IIBA) identity, recognizes a subset of Meis preboundsites that contain Hox motifs. Importantly, at these sites Meis binding is strongly increased. This enhanced Meis binding coincides with active enhancers, which are linked to genes highly expressed in the IIBA and regulated by Hoxa2. These findings show that Hoxa2 operates as a tissue-specific cofactor, enhancing Meis binding to specific sites that provide the IIBA with its anatomical identity.

AB - Hox transcription factors (TFs) are essential for vertebrate development, but how these evolutionary conserved proteins function invivo remains unclear. Because Hox proteins have notoriously low binding specificity, they are believed to bind with cofactors, mainly homeodomain TFs Pbx and Meis, to select their specific targets. We mapped binding of Meis, Pbx, and Hoxa2 in the branchial arches, a series of segments in the developing vertebrate head. Meis occupancy is largely similar in Hox-positive and -negative arches. Hoxa2, which specifies second arch (IIBA) identity, recognizes a subset of Meis preboundsites that contain Hox motifs. Importantly, at these sites Meis binding is strongly increased. This enhanced Meis binding coincides with active enhancers, which are linked to genes highly expressed in the IIBA and regulated by Hoxa2. These findings show that Hoxa2 operates as a tissue-specific cofactor, enhancing Meis binding to specific sites that provide the IIBA with its anatomical identity.

U2 - 10.1016/j.devcel.2014.12.024

DO - 10.1016/j.devcel.2014.12.024

M3 - Journal article

C2 - 25640223

AN - SCOPUS:84922804680

VL - 32

SP - 265

EP - 277

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 3

ER -

Research

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