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Human prostate tissue expresses CYP1A2 mNRA transcripts and metabolically activates potential prostate carcinogens.

Research output: Contribution to journalJournal article

Published

Journal publication date09/2000
JournalCarcinogenesis
Journal number9
Volume21
Number of pages7
Pages1683-1689
Original languageEnglish

Abstract

Epidemiological evidence suggests a link between meat consumption and prostate cancer. In this study, benign prostatic hyperplasia tissues, obtained by transurethral resection or radical retropubic prostatectomy from UK-resident individuals (n = 18), were examined for CYP1 expression and for their ability, in short-term organ culture, to metabolically activate carcinogens found in cooked meat. Semi-quantitative RT–PCR analysis of CYP1 expression detected CYP1A2 mRNA transcripts in the prostates of four individuals, as well as mRNA transcripts from CYP1A1 and CYP1B1. The compounds tested for metabolic activation were 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP; 500 µM, n = 9) and its metabolite N-hydroxy PhIP (20 µM, n = 8), 2-amino-3-methylimidazo[4,5-f]quinoline (IQ; 500 µM, n = 6) and benzo[a]pyrene (B[a]P; 50 µM, n = 5). After incubation (PFMR medium, 22 h, 37°C), DNA was isolated from tissue fragments and DNA adducts were detected and quantified by 32P-postlabelling analysis. DNA adduct formation was detected in all samples incubated with PhIP (mean, adducts per 108 nucleotides), N-hydroxy-PhIP (2736/108) or B[a]P (1/108). IQ–DNA adducts were detected in 5/6 tissues (mean, 1/108). The CYP1 inhibitor -naphthoflavone (10 µM) reduced B[a]P–DNA adduct formation in tissues from two individuals by 96 and 64%, respectively. This pilot study shows that human prostate tissue can metabolically activate `cooked meat' carcinogens, a process that could contribute to prostate cancer development.