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Identification and characterisation of a RAD51 gene from Leishmania major.

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Identification and characterisation of a RAD51 gene from Leishmania major. / McKean, P. G.; Keen, J. G.; Smith, D. F. et al.
In: Molecular and Biochemical Parasitology, Vol. 115, No. 2, 07.2001, p. 209-216.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

McKean, PG, Keen, JG, Smith, DF & Benson, FE 2001, 'Identification and characterisation of a RAD51 gene from Leishmania major.', Molecular and Biochemical Parasitology, vol. 115, no. 2, pp. 209-216. https://doi.org/10.1016/S0166-6851(01)00288-2

APA

McKean, P. G., Keen, J. G., Smith, D. F., & Benson, F. E. (2001). Identification and characterisation of a RAD51 gene from Leishmania major. Molecular and Biochemical Parasitology, 115(2), 209-216. https://doi.org/10.1016/S0166-6851(01)00288-2

Vancouver

McKean PG, Keen JG, Smith DF, Benson FE. Identification and characterisation of a RAD51 gene from Leishmania major. Molecular and Biochemical Parasitology. 2001 Jul;115(2):209-216. doi: 10.1016/S0166-6851(01)00288-2

Author

McKean, P. G. ; Keen, J. G. ; Smith, D. F. et al. / Identification and characterisation of a RAD51 gene from Leishmania major. In: Molecular and Biochemical Parasitology. 2001 ; Vol. 115, No. 2. pp. 209-216.

Bibtex

@article{734323d61eef4143a1c4d48e3d53b532,
title = "Identification and characterisation of a RAD51 gene from Leishmania major.",
abstract = "The RAD51 gene is a homologue of Escherichia coli recA which plays a central role in homologous recombination and DNA repair. This paper describes the identification of the RAD51 gene from the trypanosomatid parasite Leishmania major. The LmRAD51 gene codes for a 377 amino acid polypeptide with a predicted molecular mass of 41 259 Da that is highly homologous to the Rad51 family of proteins. Recombinant L. major Rad51 protein (LmRad51) was over-expressed in a bacterial expression system, purified to homogeneity and shown to bind DNA and exhibit DNA-stimulated ATPase activity, consistent with previously reported biochemical characteristics of Rad51 protein. Although LmRad51 expression is below the level of detection in exponentially growing cultures of Leishmania, high levels of LmRad51 mRNA and protein expression can be detected following exposure to the DNA-damaging agent phleomycin. LmRAD51 is one of the first examples of a DNA damage-inducible gene to be characterised in Leishmania, and will be invaluable in studying the contribution of homologous recombination to Leishmania virulence.",
keywords = "Rad51, Leishmania, Homologous recombination, Gene amplification",
author = "McKean, {P. G.} and Keen, {J. G.} and Smith, {D. F.} and Benson, {Fiona E.}",
year = "2001",
month = jul,
doi = "10.1016/S0166-6851(01)00288-2",
language = "English",
volume = "115",
pages = "209--216",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Identification and characterisation of a RAD51 gene from Leishmania major.

AU - McKean, P. G.

AU - Keen, J. G.

AU - Smith, D. F.

AU - Benson, Fiona E.

PY - 2001/7

Y1 - 2001/7

N2 - The RAD51 gene is a homologue of Escherichia coli recA which plays a central role in homologous recombination and DNA repair. This paper describes the identification of the RAD51 gene from the trypanosomatid parasite Leishmania major. The LmRAD51 gene codes for a 377 amino acid polypeptide with a predicted molecular mass of 41 259 Da that is highly homologous to the Rad51 family of proteins. Recombinant L. major Rad51 protein (LmRad51) was over-expressed in a bacterial expression system, purified to homogeneity and shown to bind DNA and exhibit DNA-stimulated ATPase activity, consistent with previously reported biochemical characteristics of Rad51 protein. Although LmRad51 expression is below the level of detection in exponentially growing cultures of Leishmania, high levels of LmRad51 mRNA and protein expression can be detected following exposure to the DNA-damaging agent phleomycin. LmRAD51 is one of the first examples of a DNA damage-inducible gene to be characterised in Leishmania, and will be invaluable in studying the contribution of homologous recombination to Leishmania virulence.

AB - The RAD51 gene is a homologue of Escherichia coli recA which plays a central role in homologous recombination and DNA repair. This paper describes the identification of the RAD51 gene from the trypanosomatid parasite Leishmania major. The LmRAD51 gene codes for a 377 amino acid polypeptide with a predicted molecular mass of 41 259 Da that is highly homologous to the Rad51 family of proteins. Recombinant L. major Rad51 protein (LmRad51) was over-expressed in a bacterial expression system, purified to homogeneity and shown to bind DNA and exhibit DNA-stimulated ATPase activity, consistent with previously reported biochemical characteristics of Rad51 protein. Although LmRad51 expression is below the level of detection in exponentially growing cultures of Leishmania, high levels of LmRad51 mRNA and protein expression can be detected following exposure to the DNA-damaging agent phleomycin. LmRAD51 is one of the first examples of a DNA damage-inducible gene to be characterised in Leishmania, and will be invaluable in studying the contribution of homologous recombination to Leishmania virulence.

KW - Rad51

KW - Leishmania

KW - Homologous recombination

KW - Gene amplification

U2 - 10.1016/S0166-6851(01)00288-2

DO - 10.1016/S0166-6851(01)00288-2

M3 - Journal article

VL - 115

SP - 209

EP - 216

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 2

ER -