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Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella. / Harvey-Samuel, T.; Norman, V.C.; Carter, R. et al.
In: Insect Molecular Biology, Vol. 29, No. 2, 01.04.2020, p. 231-240.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Harvey-Samuel, T, Norman, VC, Carter, R, Lovett, E & Alphey, L 2020, 'Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella', Insect Molecular Biology, vol. 29, no. 2, pp. 231-240. https://doi.org/10.1111/imb.12628

APA

Harvey-Samuel, T., Norman, V. C., Carter, R., Lovett, E., & Alphey, L. (2020). Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella. Insect Molecular Biology, 29(2), 231-240. https://doi.org/10.1111/imb.12628

Vancouver

Harvey-Samuel T, Norman VC, Carter R, Lovett E, Alphey L. Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella. Insect Molecular Biology. 2020 Apr 1;29(2):231-240. Epub 2019 Dec 19. doi: 10.1111/imb.12628

Author

Harvey-Samuel, T. ; Norman, V.C. ; Carter, R. et al. / Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella. In: Insect Molecular Biology. 2020 ; Vol. 29, No. 2. pp. 231-240.

Bibtex

@article{7adeba2dadcf4d338ad4fa8045b406ce,
title = "Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella",
abstract = "Recently, a novel sex‐determination system was identified in the silkworm (Bombyx mori) in which a piwi‐interacting RNA (piRNA) encoded on the female‐specific W chromosome silences a Z‐linked gene (Masculinizer) that would otherwise initiate male sex‐determination and dosage compensation. Masculinizer provides various opportunities for developing improved genetic pest management tools. A pest lepidopteran in which a genetic pest management system has been developed, but which would benefit greatly from such improved designs, is the diamondback moth, Plutella xylostella. However, Masculinizer has not yet been identified in this species. Here, focusing on the previously described {\textquoteleft}masculinizing{\textquoteright} domain of B. mori Masculinizer, we identify P. xylostella Masculinizer (PxyMasc). We show that PxyMasc is Z‐linked, regulates sex‐specific alternative splicing of doublesex and is necessary for male survival. Similar results in B. mori suggest this survival effect is possibly through failure to initiate male dosage compensation. The highly conserved function and location of this gene between these two distantly related lepidopterans suggests a deep role for Masculinizer in the sex‐determination systems of the Lepidoptera.",
author = "T. Harvey-Samuel and V.C. Norman and R. Carter and E. Lovett and L. Alphey",
note = "Export Date: 5 February 2020",
year = "2020",
month = apr,
day = "1",
doi = "10.1111/imb.12628",
language = "English",
volume = "29",
pages = "231--240",
journal = "Insect Molecular Biology",
issn = "0962-1075",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella

AU - Harvey-Samuel, T.

AU - Norman, V.C.

AU - Carter, R.

AU - Lovett, E.

AU - Alphey, L.

N1 - Export Date: 5 February 2020

PY - 2020/4/1

Y1 - 2020/4/1

N2 - Recently, a novel sex‐determination system was identified in the silkworm (Bombyx mori) in which a piwi‐interacting RNA (piRNA) encoded on the female‐specific W chromosome silences a Z‐linked gene (Masculinizer) that would otherwise initiate male sex‐determination and dosage compensation. Masculinizer provides various opportunities for developing improved genetic pest management tools. A pest lepidopteran in which a genetic pest management system has been developed, but which would benefit greatly from such improved designs, is the diamondback moth, Plutella xylostella. However, Masculinizer has not yet been identified in this species. Here, focusing on the previously described ‘masculinizing’ domain of B. mori Masculinizer, we identify P. xylostella Masculinizer (PxyMasc). We show that PxyMasc is Z‐linked, regulates sex‐specific alternative splicing of doublesex and is necessary for male survival. Similar results in B. mori suggest this survival effect is possibly through failure to initiate male dosage compensation. The highly conserved function and location of this gene between these two distantly related lepidopterans suggests a deep role for Masculinizer in the sex‐determination systems of the Lepidoptera.

AB - Recently, a novel sex‐determination system was identified in the silkworm (Bombyx mori) in which a piwi‐interacting RNA (piRNA) encoded on the female‐specific W chromosome silences a Z‐linked gene (Masculinizer) that would otherwise initiate male sex‐determination and dosage compensation. Masculinizer provides various opportunities for developing improved genetic pest management tools. A pest lepidopteran in which a genetic pest management system has been developed, but which would benefit greatly from such improved designs, is the diamondback moth, Plutella xylostella. However, Masculinizer has not yet been identified in this species. Here, focusing on the previously described ‘masculinizing’ domain of B. mori Masculinizer, we identify P. xylostella Masculinizer (PxyMasc). We show that PxyMasc is Z‐linked, regulates sex‐specific alternative splicing of doublesex and is necessary for male survival. Similar results in B. mori suggest this survival effect is possibly through failure to initiate male dosage compensation. The highly conserved function and location of this gene between these two distantly related lepidopterans suggests a deep role for Masculinizer in the sex‐determination systems of the Lepidoptera.

U2 - 10.1111/imb.12628

DO - 10.1111/imb.12628

M3 - Journal article

VL - 29

SP - 231

EP - 240

JO - Insect Molecular Biology

JF - Insect Molecular Biology

SN - 0962-1075

IS - 2

ER -