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Identification of Benzo[a]pyrene-metabolizing bacteria in forest soils by using DNA-based stable-isotope probing

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Identification of Benzo[a]pyrene-metabolizing bacteria in forest soils by using DNA-based stable-isotope probing. / Song, Mengke; Luo, Chunling; Jiang, Longfei; Zhang, Dayi; Wang, Yujie; Zhang, Gan.

In: Applied and Environmental Microbiology, Vol. 81, No. 21, 11.2015, p. 7368-7376.

Research output: Contribution to journalJournal article

Harvard

Song, M, Luo, C, Jiang, L, Zhang, D, Wang, Y & Zhang, G 2015, 'Identification of Benzo[a]pyrene-metabolizing bacteria in forest soils by using DNA-based stable-isotope probing', Applied and Environmental Microbiology, vol. 81, no. 21, pp. 7368-7376. https://doi.org/10.1128/AEM.01983-15

APA

Song, M., Luo, C., Jiang, L., Zhang, D., Wang, Y., & Zhang, G. (2015). Identification of Benzo[a]pyrene-metabolizing bacteria in forest soils by using DNA-based stable-isotope probing. Applied and Environmental Microbiology, 81(21), 7368-7376. https://doi.org/10.1128/AEM.01983-15

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Author

Song, Mengke ; Luo, Chunling ; Jiang, Longfei ; Zhang, Dayi ; Wang, Yujie ; Zhang, Gan. / Identification of Benzo[a]pyrene-metabolizing bacteria in forest soils by using DNA-based stable-isotope probing. In: Applied and Environmental Microbiology. 2015 ; Vol. 81, No. 21. pp. 7368-7376.

Bibtex

@article{5506a535cd33483581feceb9229cc92a,
title = "Identification of Benzo[a]pyrene-metabolizing bacteria in forest soils by using DNA-based stable-isotope probing",
abstract = "DNA-based stable-isotope probing (DNA-SIP) was used in this study to investigate the uncultivated bacteria with benzo[a]pyrene (BaP) metabolism capacities in two Chinese forest soils (Mt. Maoer in Heilongjiang Province and Mt. Baicaowa in Hubei Province). We characterized three different phylotypes with responsibility for BaP degradation, none of which were previously reported as BaP-degrading microorganisms by SIP. In Mt. Maoer soil microcosms, the putative BaP degraders were classified as belonging to the genus Terrimonas (family Chitinophagaceae, order Sphingobacteriales), whereas Burkholderia spp. were the key BaP degraders in Mt. Baicaowa soils. The addition of metabolic salicylate significantly increased BaP degradation efficiency in Mt. Maoer soils, and the BaP-metabolizing bacteria shifted to the microorganisms in the family Oxalobacteraceae (genus unclassified). Meanwhile, salicylate addition did not change either BaP degradation or putative BaP degraders in Mt. Baicaowa. Polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHD) genes were amplified, sequenced, and quantified in the DNA-SIP (13)C heavy fraction to further confirm the BaP metabolism. By illuminating the microbial diversity and salicylate additive effects on BaP degradation across different soils, the results increased our understanding of BaP natural attenuation and provided a possible approach to enhance the bioremediation of BaP-contaminated soils.",
author = "Mengke Song and Chunling Luo and Longfei Jiang and Dayi Zhang and Yujie Wang and Gan Zhang",
note = "Copyright {\textcopyright} 2015 by the American Society for Microbiology",
year = "2015",
month = nov
doi = "10.1128/AEM.01983-15",
language = "English",
volume = "81",
pages = "7368--7376",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "21",

}

RIS

TY - JOUR

T1 - Identification of Benzo[a]pyrene-metabolizing bacteria in forest soils by using DNA-based stable-isotope probing

AU - Song, Mengke

AU - Luo, Chunling

AU - Jiang, Longfei

AU - Zhang, Dayi

AU - Wang, Yujie

AU - Zhang, Gan

N1 - Copyright © 2015 by the American Society for Microbiology

PY - 2015/11

Y1 - 2015/11

N2 - DNA-based stable-isotope probing (DNA-SIP) was used in this study to investigate the uncultivated bacteria with benzo[a]pyrene (BaP) metabolism capacities in two Chinese forest soils (Mt. Maoer in Heilongjiang Province and Mt. Baicaowa in Hubei Province). We characterized three different phylotypes with responsibility for BaP degradation, none of which were previously reported as BaP-degrading microorganisms by SIP. In Mt. Maoer soil microcosms, the putative BaP degraders were classified as belonging to the genus Terrimonas (family Chitinophagaceae, order Sphingobacteriales), whereas Burkholderia spp. were the key BaP degraders in Mt. Baicaowa soils. The addition of metabolic salicylate significantly increased BaP degradation efficiency in Mt. Maoer soils, and the BaP-metabolizing bacteria shifted to the microorganisms in the family Oxalobacteraceae (genus unclassified). Meanwhile, salicylate addition did not change either BaP degradation or putative BaP degraders in Mt. Baicaowa. Polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHD) genes were amplified, sequenced, and quantified in the DNA-SIP (13)C heavy fraction to further confirm the BaP metabolism. By illuminating the microbial diversity and salicylate additive effects on BaP degradation across different soils, the results increased our understanding of BaP natural attenuation and provided a possible approach to enhance the bioremediation of BaP-contaminated soils.

AB - DNA-based stable-isotope probing (DNA-SIP) was used in this study to investigate the uncultivated bacteria with benzo[a]pyrene (BaP) metabolism capacities in two Chinese forest soils (Mt. Maoer in Heilongjiang Province and Mt. Baicaowa in Hubei Province). We characterized three different phylotypes with responsibility for BaP degradation, none of which were previously reported as BaP-degrading microorganisms by SIP. In Mt. Maoer soil microcosms, the putative BaP degraders were classified as belonging to the genus Terrimonas (family Chitinophagaceae, order Sphingobacteriales), whereas Burkholderia spp. were the key BaP degraders in Mt. Baicaowa soils. The addition of metabolic salicylate significantly increased BaP degradation efficiency in Mt. Maoer soils, and the BaP-metabolizing bacteria shifted to the microorganisms in the family Oxalobacteraceae (genus unclassified). Meanwhile, salicylate addition did not change either BaP degradation or putative BaP degraders in Mt. Baicaowa. Polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHD) genes were amplified, sequenced, and quantified in the DNA-SIP (13)C heavy fraction to further confirm the BaP metabolism. By illuminating the microbial diversity and salicylate additive effects on BaP degradation across different soils, the results increased our understanding of BaP natural attenuation and provided a possible approach to enhance the bioremediation of BaP-contaminated soils.

U2 - 10.1128/AEM.01983-15

DO - 10.1128/AEM.01983-15

M3 - Journal article

C2 - 26253666

VL - 81

SP - 7368

EP - 7376

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 21

ER -