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IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes

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IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes. / Almine, Jessica F.; O'Hare, Craig A. J.; Dunphy, Gillian; Haga, Ismar R.; Naik, Rangeetha J.; Atrih, Abdelmadjid; Connolly, Dympna J.; Taylor, Jordan; Kelsall, Ian R.; Bowie, Andrew G.; Beard, Philippa M.; Unterholzner, Leonie.

In: Nature Communications, Vol. 8, 14392, 13.02.2017.

Research output: Contribution to journalJournal article

Harvard

Almine, JF, O'Hare, CAJ, Dunphy, G, Haga, IR, Naik, RJ, Atrih, A, Connolly, DJ, Taylor, J, Kelsall, IR, Bowie, AG, Beard, PM & Unterholzner, L 2017, 'IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes', Nature Communications, vol. 8, 14392. https://doi.org/10.1038/ncomms14392

APA

Almine, J. F., O'Hare, C. A. J., Dunphy, G., Haga, I. R., Naik, R. J., Atrih, A., Connolly, D. J., Taylor, J., Kelsall, I. R., Bowie, A. G., Beard, P. M., & Unterholzner, L. (2017). IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes. Nature Communications, 8, [14392]. https://doi.org/10.1038/ncomms14392

Vancouver

Almine JF, O'Hare CAJ, Dunphy G, Haga IR, Naik RJ, Atrih A et al. IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes. Nature Communications. 2017 Feb 13;8. 14392. https://doi.org/10.1038/ncomms14392

Author

Almine, Jessica F. ; O'Hare, Craig A. J. ; Dunphy, Gillian ; Haga, Ismar R. ; Naik, Rangeetha J. ; Atrih, Abdelmadjid ; Connolly, Dympna J. ; Taylor, Jordan ; Kelsall, Ian R. ; Bowie, Andrew G. ; Beard, Philippa M. ; Unterholzner, Leonie. / IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes. In: Nature Communications. 2017 ; Vol. 8.

Bibtex

@article{99a0d15f87cc46ffa94ec803c5a00ed6,
title = "IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes",
abstract = "Many human cells can sense the presence of exogenous DNA during infection though the cytosolic DNA receptor cyclic GMP-AMP synthase (cGAS), which produces the second messenger cyclic GMP-AMP (cGAMP). Other putative DNA receptors have been described, but whether their functions are redundant, tissue-specific or integrated in the cGAS-cGAMP pathway is unclear. Here we show that interferon-γ inducible protein 16 (IFI16) cooperates with cGAS during DNA sensing in human keratinocytes, as both cGAS and IFI16 are required for the full activation of an innate immune response to exogenous DNA and DNA viruses. IFI16 is also required for the cGAMP-induced activation of STING, and interacts with STING to promote STING phosphorylation and translocation. We propose that the two DNA sensors IFI16 and cGAS cooperate to prevent the spurious activation of the type I interferon response.",
author = "Almine, {Jessica F.} and O'Hare, {Craig A. J.} and Gillian Dunphy and Haga, {Ismar R.} and Naik, {Rangeetha J.} and Abdelmadjid Atrih and Connolly, {Dympna J.} and Jordan Taylor and Kelsall, {Ian R.} and Bowie, {Andrew G.} and Beard, {Philippa M.} and Leonie Unterholzner",
year = "2017",
month = feb,
day = "13",
doi = "10.1038/ncomms14392",
language = "English",
volume = "8",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes

AU - Almine, Jessica F.

AU - O'Hare, Craig A. J.

AU - Dunphy, Gillian

AU - Haga, Ismar R.

AU - Naik, Rangeetha J.

AU - Atrih, Abdelmadjid

AU - Connolly, Dympna J.

AU - Taylor, Jordan

AU - Kelsall, Ian R.

AU - Bowie, Andrew G.

AU - Beard, Philippa M.

AU - Unterholzner, Leonie

PY - 2017/2/13

Y1 - 2017/2/13

N2 - Many human cells can sense the presence of exogenous DNA during infection though the cytosolic DNA receptor cyclic GMP-AMP synthase (cGAS), which produces the second messenger cyclic GMP-AMP (cGAMP). Other putative DNA receptors have been described, but whether their functions are redundant, tissue-specific or integrated in the cGAS-cGAMP pathway is unclear. Here we show that interferon-γ inducible protein 16 (IFI16) cooperates with cGAS during DNA sensing in human keratinocytes, as both cGAS and IFI16 are required for the full activation of an innate immune response to exogenous DNA and DNA viruses. IFI16 is also required for the cGAMP-induced activation of STING, and interacts with STING to promote STING phosphorylation and translocation. We propose that the two DNA sensors IFI16 and cGAS cooperate to prevent the spurious activation of the type I interferon response.

AB - Many human cells can sense the presence of exogenous DNA during infection though the cytosolic DNA receptor cyclic GMP-AMP synthase (cGAS), which produces the second messenger cyclic GMP-AMP (cGAMP). Other putative DNA receptors have been described, but whether their functions are redundant, tissue-specific or integrated in the cGAS-cGAMP pathway is unclear. Here we show that interferon-γ inducible protein 16 (IFI16) cooperates with cGAS during DNA sensing in human keratinocytes, as both cGAS and IFI16 are required for the full activation of an innate immune response to exogenous DNA and DNA viruses. IFI16 is also required for the cGAMP-induced activation of STING, and interacts with STING to promote STING phosphorylation and translocation. We propose that the two DNA sensors IFI16 and cGAS cooperate to prevent the spurious activation of the type I interferon response.

U2 - 10.1038/ncomms14392

DO - 10.1038/ncomms14392

M3 - Journal article

C2 - 28194029

VL - 8

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 14392

ER -