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  • CHEM34688R1-2

    Rights statement: This is the author’s version of a work that was accepted for publication in Chemosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemosphere, 138, 2015 DOI: 10.1016/j.chemosphere.2015.06.018

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Immobilization of Shewanella oneidensis MR-1 in diffusive gradients in thin films for determining metal bioavailability

Research output: Contribution to journalJournal article

Published
<mark>Journal publication date</mark>11/2015
<mark>Journal</mark>Chemosphere
Volume138
Number of pages7
Pages (from-to)309-315
Publication statusPublished
Early online date17/06/15
Original languageEnglish

Abstract

Assessing metal bioavailability in soil is important in modelling the effects of metal toxicity on the surrounding ecosystem. Current methods based on diffusive gradient thin films (DGTs) and Gel-Integrated Microelectrode are limited in their availability and sensitivity. To address this, S. oneidensis, an anaerobic iron reducing bacterium, was incorporated into a thin layer of agarose to replace the polyacrylamide gel that is normally present in DGT to form biologically mobilizing DGT (BMDGT). Viability analysis revealed that 16-35% of the cells remained viable within the BMDGTs depending on the culturing conditions over a 20 h period with/without metals. Deployment of BMDGTs in standardized metal solutions showed significant differences to cell
free BMDGTs when cells grown in Luria Broth (LB) were incorporated into BMDGTs and deployed under anaerobic conditions. Deployment of these BMDGTs in hematite revealed no significant differences between BMDGTs and BMDGTs containing heat killed cells. Whether heat killed cells retain the ability to affect bioavailability is uncertain. This is the first study to investigate how a microorganism that was incorporated into a DGT device such as the metal reducing bacteria, S. oneidensis, may affect the mobility of metals.

Bibliographic note

This is the author’s version of a work that was accepted for publication in Chemosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemosphere, 138, 2015 DOI: 10.1016/j.chemosphere.2015.06.018 From: ees.chem.0.31d356.ae2990e7@eesmail.elsevier.com [mailto:ees.chem.0.31d356.ae2990e7@eesmail.elsevier.com] On Behalf Of Chemosphere Sent: 10 June 2015 04:31 To: Paul Baker; bakerpaulw@gmail.com Subject: CHEM34688R1 - Editor decision - accepted Dear Dr. Baker, I am pleased to inform you that the manuscript "Immobilization of Shewanella oneidensis MR-1 in diffusive gradients in thin films for determining metal bioavailability" (Dr. Paul William Baker) has now been accepted by the editor for publication. Your manuscript will soon be passed to the production department for further handling. Then you will receive further notice. When your paper is published on ScienceDirect, you want to make sure it gets the attention it deserves. To help you get your message across, Elsevier has developed a new, free service called AudioSlides: brief, webcast-style presentations that are shown (publicly available) next to your published article. This format gives you the opportunity to explain your research in your own words and attract interest. You will receive an invitation email to create an AudioSlides presentation shortly. For more information and examples, please visit http://www.elsevier.com/audioslides. Thank you for considering our journal for the publication of your research. Kind regards, Prof. Xinde Cao Editor Chemosphere