Rights statement: http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 116 (4), pp 305-309 1998, © 1998 Cambridge University Press.
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana
AU - Zakai, H A
AU - Chance, M L
AU - Bates, P A
N1 - http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 116 (4), pp 305-309 1998, © 1998 Cambridge University Press.
PY - 1998/4
Y1 - 1998/4
N2 - Promastigotes of Leishmania braziliensis, L. donovani, L. major and L. mexicana recently derived from tissue amastigotes were cultured in Schneider's Drosophila medium supplemented with 20% (v/v) heat-inactivated foetal calf serum and 25 micrograms gentamicin sulfate/ml at pH 5.5. These cultures produced more metacyclic promastigotes in their stationary-phase populations than others cultured at pH 7.0. Metacyclic promastigotes possessed a short (<or = 8 microns) and narrow (<or = 1.5 microns) cell body with a flagellum twice or more the length of the cell body. Promastigotes from acidic cultures were more resistant to complement-mediated lysis and more infective in vivo than those grown at neutral pH. These results demonstrate that induction of metacyclogenesis by acidic pH is a response conserved across a variety of species of Leishmania.
AB - Promastigotes of Leishmania braziliensis, L. donovani, L. major and L. mexicana recently derived from tissue amastigotes were cultured in Schneider's Drosophila medium supplemented with 20% (v/v) heat-inactivated foetal calf serum and 25 micrograms gentamicin sulfate/ml at pH 5.5. These cultures produced more metacyclic promastigotes in their stationary-phase populations than others cultured at pH 7.0. Metacyclic promastigotes possessed a short (<or = 8 microns) and narrow (<or = 1.5 microns) cell body with a flagellum twice or more the length of the cell body. Promastigotes from acidic cultures were more resistant to complement-mediated lysis and more infective in vivo than those grown at neutral pH. These results demonstrate that induction of metacyclogenesis by acidic pH is a response conserved across a variety of species of Leishmania.
KW - Leishmania spp
KW - promastigote
KW - metacyclic
KW - culture
KW - infectivity
KW - differentiation
M3 - Journal article
C2 - 9585932
VL - 116
SP - 305
EP - 309
JO - Parasitology
JF - Parasitology
SN - 0031-1820
IS - 4
ER -