Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Interactions of multiwalled carbon nanotubes with algal cells
T2 - quantification of association, visualization of uptake, and measurement of alterations in the composition of cells
AU - Rhiem, Stefan
AU - Riding, Matthew J.
AU - Baumgartner, Werner
AU - Martin, Francis L.
AU - Semple, Kirk T.
AU - Jones, Kevin C.
AU - Schäffer, Andreas
AU - Maes, Hanna M.
PY - 2015/1
Y1 - 2015/1
N2 - Carbon nanotubes (CNTs) are considered promising materials in nanotechnology. We quantified CNT accumulation by the alga Desmodesmus subspicatus. Cells were exposed to radiolabeled CNTs ((14)C-CNTs; 1 mg/L) to determine uptake and association, as well as elimination and dissociation in clear media. Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) was used to detect effects of CNTs on algae. CNT-cell interactions were visualized by electron microscopy and related to alterations in their cell composition. A concentration factor of 5000 L/kg dry weight was calculated. Most of the material agglomerated around the cells, but single tubes were detected in the cytoplasm. Computational analyses of the ATR-FTIR data showed that CNT treated algae differed from controls at all sampling times. CNT exposure changed the biochemical composition of cells. The fact that CNTs are bioavailable for algae and that they influence the cell composition is important with regard to environmental risk assessment of this nanomaterial.
AB - Carbon nanotubes (CNTs) are considered promising materials in nanotechnology. We quantified CNT accumulation by the alga Desmodesmus subspicatus. Cells were exposed to radiolabeled CNTs ((14)C-CNTs; 1 mg/L) to determine uptake and association, as well as elimination and dissociation in clear media. Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) was used to detect effects of CNTs on algae. CNT-cell interactions were visualized by electron microscopy and related to alterations in their cell composition. A concentration factor of 5000 L/kg dry weight was calculated. Most of the material agglomerated around the cells, but single tubes were detected in the cytoplasm. Computational analyses of the ATR-FTIR data showed that CNT treated algae differed from controls at all sampling times. CNT exposure changed the biochemical composition of cells. The fact that CNTs are bioavailable for algae and that they influence the cell composition is important with regard to environmental risk assessment of this nanomaterial.
KW - Carbon nanotubes
KW - Green algae
KW - Bioavailability
KW - Electron microscopy
KW - FTIR spectroscopy
KW - Nanoparticles
U2 - 10.1016/j.envpol.2014.11.011
DO - 10.1016/j.envpol.2014.11.011
M3 - Journal article
C2 - 25467692
VL - 196
SP - 431
EP - 439
JO - Environmental Pollution
JF - Environmental Pollution
SN - 0269-7491
ER -