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Isolating HeLa cell fractions enriched for clathrin-coated vesicles

Research output: Contribution to journalJournal articlepeer-review

Published
<mark>Journal publication date</mark>3/11/2014
<mark>Journal</mark>Cold Spring Harbor Protocols
Issue number11
Volume2014
Number of pages4
Pages (from-to)1184-1187
Publication StatusPublished
<mark>Original language</mark>English

Abstract

HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy.