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Isolating HeLa cell fractions enriched for clathrin-coated vesicles

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Isolating HeLa cell fractions enriched for clathrin-coated vesicles. / Borner, Georg H H; Fielding, Andrew B.
In: Cold Spring Harbor Protocols, Vol. 2014, No. 11, 03.11.2014, p. 1184-1187.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Borner, GHH & Fielding, AB 2014, 'Isolating HeLa cell fractions enriched for clathrin-coated vesicles', Cold Spring Harbor Protocols, vol. 2014, no. 11, pp. 1184-1187. https://doi.org/10.1101/pdb.prot083147

APA

Borner, G. H. H., & Fielding, A. B. (2014). Isolating HeLa cell fractions enriched for clathrin-coated vesicles. Cold Spring Harbor Protocols, 2014(11), 1184-1187. https://doi.org/10.1101/pdb.prot083147

Vancouver

Borner GHH, Fielding AB. Isolating HeLa cell fractions enriched for clathrin-coated vesicles. Cold Spring Harbor Protocols. 2014 Nov 3;2014(11):1184-1187. doi: 10.1101/pdb.prot083147

Author

Borner, Georg H H ; Fielding, Andrew B. / Isolating HeLa cell fractions enriched for clathrin-coated vesicles. In: Cold Spring Harbor Protocols. 2014 ; Vol. 2014, No. 11. pp. 1184-1187.

Bibtex

@article{144ff13c59d1404ca95b6118ec3a18dc,
title = "Isolating HeLa cell fractions enriched for clathrin-coated vesicles",
abstract = "HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy.",
keywords = "Blotting, Western, Cell Fractionation, Clathrin-Coated Vesicles, HeLa Cells, Humans, Mass Spectrometry, Microscopy, Electron, Journal Article",
author = "Borner, {Georg H H} and Fielding, {Andrew B.}",
note = "{\textcopyright} 2014 Cold Spring Harbor Laboratory Press.",
year = "2014",
month = nov,
day = "3",
doi = "10.1101/pdb.prot083147",
language = "English",
volume = "2014",
pages = "1184--1187",
journal = "Cold Spring Harbor Protocols",
issn = "1940-3402",
publisher = "Cold Spring Harbor Laboratory Press",
number = "11",

}

RIS

TY - JOUR

T1 - Isolating HeLa cell fractions enriched for clathrin-coated vesicles

AU - Borner, Georg H H

AU - Fielding, Andrew B.

N1 - © 2014 Cold Spring Harbor Laboratory Press.

PY - 2014/11/3

Y1 - 2014/11/3

N2 - HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy.

AB - HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy.

KW - Blotting, Western

KW - Cell Fractionation

KW - Clathrin-Coated Vesicles

KW - HeLa Cells

KW - Humans

KW - Mass Spectrometry

KW - Microscopy, Electron

KW - Journal Article

U2 - 10.1101/pdb.prot083147

DO - 10.1101/pdb.prot083147

M3 - Journal article

C2 - 25368312

VL - 2014

SP - 1184

EP - 1187

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1940-3402

IS - 11

ER -