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Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720.

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Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720. / Onek, Leonzio A.; Lea, Peter J.; Smith, R. John.
In: Archives of Microbiology, Vol. 161, No. 4, 1994, p. 352-358.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Onek, LA, Lea, PJ & Smith, RJ 1994, 'Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720.', Archives of Microbiology, vol. 161, no. 4, pp. 352-358. https://doi.org/10.1007/BF00303592

APA

Onek, L. A., Lea, P. J., & Smith, R. J. (1994). Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720. Archives of Microbiology, 161(4), 352-358. https://doi.org/10.1007/BF00303592

Vancouver

Onek LA, Lea PJ, Smith RJ. Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720. Archives of Microbiology. 1994;161(4):352-358. doi: 10.1007/BF00303592

Author

Onek, Leonzio A. ; Lea, Peter J. ; Smith, R. John. / Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720. In: Archives of Microbiology. 1994 ; Vol. 161, No. 4. pp. 352-358.

Bibtex

@article{dff19ab135774759886b40cce257654a,
title = "Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720.",
abstract = "A 21-kDa novel polypeptide which possesses characteristics normally considered to be diagnostic of the calmodulin present in eukaryotic cells was isolated from the cyanobacterium Nostoc sp. PCC 6720. The major technique employed in the isolation of the polypeptide was ion-exchange chromatography on a Mono Q column. The 21-kDa polypeptide was shown: to activate pea NAD kinase in vitro, in a Ca2+ requiring reaction; to react with polyclonal antibodies raised against spinach calmodulin, but not with those raised against bovine brain calmodulin; and to exhibit a Ca2+ dependent shift in migration during SDS-PAGE.",
keywords = "Calcium - binding protein - Calmodulin - Nostoc sp. PCC 6720",
author = "Onek, {Leonzio A.} and Lea, {Peter J.} and Smith, {R. John}",
year = "1994",
doi = "10.1007/BF00303592",
language = "English",
volume = "161",
pages = "352--358",
journal = "Archives of Microbiology",
issn = "0302-8933",
publisher = "Springer Verlag",
number = "4",

}

RIS

TY - JOUR

T1 - Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720.

AU - Onek, Leonzio A.

AU - Lea, Peter J.

AU - Smith, R. John

PY - 1994

Y1 - 1994

N2 - A 21-kDa novel polypeptide which possesses characteristics normally considered to be diagnostic of the calmodulin present in eukaryotic cells was isolated from the cyanobacterium Nostoc sp. PCC 6720. The major technique employed in the isolation of the polypeptide was ion-exchange chromatography on a Mono Q column. The 21-kDa polypeptide was shown: to activate pea NAD kinase in vitro, in a Ca2+ requiring reaction; to react with polyclonal antibodies raised against spinach calmodulin, but not with those raised against bovine brain calmodulin; and to exhibit a Ca2+ dependent shift in migration during SDS-PAGE.

AB - A 21-kDa novel polypeptide which possesses characteristics normally considered to be diagnostic of the calmodulin present in eukaryotic cells was isolated from the cyanobacterium Nostoc sp. PCC 6720. The major technique employed in the isolation of the polypeptide was ion-exchange chromatography on a Mono Q column. The 21-kDa polypeptide was shown: to activate pea NAD kinase in vitro, in a Ca2+ requiring reaction; to react with polyclonal antibodies raised against spinach calmodulin, but not with those raised against bovine brain calmodulin; and to exhibit a Ca2+ dependent shift in migration during SDS-PAGE.

KW - Calcium - binding protein - Calmodulin - Nostoc sp. PCC 6720

U2 - 10.1007/BF00303592

DO - 10.1007/BF00303592

M3 - Journal article

VL - 161

SP - 352

EP - 358

JO - Archives of Microbiology

JF - Archives of Microbiology

SN - 0302-8933

IS - 4

ER -